C57BL/6JCya-Meiobem1/Cya
Common Name:
Meiob-KO
Product ID:
S-KO-20375
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Meiob-KO
Strain ID
KOCMP-75178-Meiob-B6J-VB
Gene Name
Product ID
S-KO-20375
Gene Alias
4930528F23Rik; MLZ-675
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Meiobem1/Cya mice (Catalog S-KO-20375) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024972
NCBI RefSeq
NM_029197
Target Region
Exon 3~11
Size of Effective Region
~19.7 kb
Detailed Document
Overview of Gene Research
Meiob, short for meiosis specific with OB domain, is a crucial gene in meiotic homologous recombination [4]. It codes for a single-strand DNA binding protein essential for DNA double-strand breaks repair during meiosis [3]. Meiob is specifically expressed in early meiotic germ cells and is involved in processes like homologous chromosome synapsis [4]. The protein forms a complex with RPA and SPATA22, localizing to meiotic chromosomes [5].
Meiob-null mouse mutants are infertile in both sexes due to meiotic arrest at a zygotene/pachytene-like stage [4,5]. In vitro functional analyses of Meiob variants in human patients also revealed truncated proteins and impaired function, leading to conditions like primary ovarian insufficiency (POI) and non-obstructive azoospermia (NOA) [1,2]. In NOA patients, Meiob pathogenic variants caused meiotic arrest, similar to the phenotype in Meiob mutant mice [2].
In conclusion, Meiob is essential for meiotic recombination and chromosomal synapsis. Studies on Meiob-null mouse models and human patients with Meiob variants have significantly enhanced our understanding of its role in fertility-related diseases such as POI and NOA.
References：
1. Wang, Yurong, Liu, Ling, Tan, Chen, He, Wen-Bin, Tan, Yue-Qiu. 2022. Novel MEIOB variants cause primary ovarian insufficiency and non-obstructive azoospermia. In Frontiers in genetics, 13, 936264. doi:10.3389/fgene.2022.936264. https://pubmed.ncbi.nlm.nih.gov/35991565/
2. Zhu, Xiaoyu, Hu, Kaiqin, Cheng, Huiru, Tang, Dongdong, Guo, Rui. 2023. Novel MEIOB pathogenic variants including a homozygous non-canonical splicing variant, cause meiotic arrest and human non-obstructive azoospermia. In Clinical genetics, 105, 99-105. doi:10.1111/cge.14426. https://pubmed.ncbi.nlm.nih.gov/37715646/
3. Gershoni, Moran, Hauser, Ron, Barda, Shimi, Pietrokovski, Shmuel, Kleiman, Sandra E. . A new MEIOB mutation is a recurrent cause for azoospermia and testicular meiotic arrest. In Human reproduction (Oxford, England), 34, 666-671. doi:10.1093/humrep/dez016. https://pubmed.ncbi.nlm.nih.gov/30838384/
4. Souquet, Benoit, Abby, Emilie, Hervé, Roxane, Habert, René, Livera, Gabriel. 2013. MEIOB targets single-strand DNA and is necessary for meiotic recombination. In PLoS genetics, 9, e1003784. doi:10.1371/journal.pgen.1003784. https://pubmed.ncbi.nlm.nih.gov/24068956/
5. Luo, Mengcheng, Yang, Fang, Leu, N Adrian, La Salle, Sophie, Wang, P Jeremy. . MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. In Nature communications, 4, 2788. doi:10.1038/ncomms3788. https://pubmed.ncbi.nlm.nih.gov/24240703/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen