C57BL/6JCya-Mir10aem1/Cya
Common Name:
Mir10a-KO
Product ID:
S-KO-20376
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mir10a-KO
Strain ID
KOCMP-723893-Mir10a-B6J-VA
Gene Name
Product ID
S-KO-20376
Gene Alias
Mirn10a; miR-10a; mmu-mir-10a
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir10aem1/Cya mice (Catalog S-KO-20376) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083585
NCBI RefSeq
NR_029784
Target Region
Exon 1
Size of Effective Region
~0.5 kb
Detailed Document
Overview of Gene Research
Mir10a, a micro-RNA gene, plays significant roles in multiple biological processes and disease conditions. It is involved in various regulatory pathways, with its expression being linked to the development and progression of different diseases. Micro-RNAs like Mir10a fine-tune gene expression by pairing with mRNAs of protein-coding genes, thus influencing post-transcriptional repression [3,5].
In B-cell lymphoma, the chromosomal aberration t(14;17)(q32;q21) activates Mir10a, and it is proposed to play an oncogenic role by inhibiting BCL6 in later stages of lymphomagenesis [1]. In human gliomas, TGF-β induces Mir10a expression, and this promotes cell migration through the suppression of PTEN [2]. In ovarian clear-cell carcinoma, Mir10a-5p is significantly upregulated, and its overexpression leads to decreased cell proliferation and a cell-cycle shift, targeting genes involved in proliferation and cell-cycle regulation [4].
In conclusion, Mir10a is a crucial micro-RNA involved in the regulation of cell-cycle, migration, and proliferation, with implications in diseases such as lymphoma, gliomas, and ovarian clear-cell carcinoma. Understanding the role of Mir10a through in-vivo studies, potentially including gene knockout models, can provide insights into disease mechanisms and may offer new therapeutic strategies for these diseases.
References:
1. Nagel, Stefan, Pommerenke, Claudia, Meyer, Corinna, Kaufmann, Maren, MacLeod, Roderick A F. 2023. Chromosomal Aberration t(14;17)(q32;q21) Simultaneously Activates HOXB5 and miR10a in Triple-Hit B-Cell Lymphoma. In Biomedicines, 11, . doi:10.3390/biomedicines11061758. https://pubmed.ncbi.nlm.nih.gov/37371852/
2. Liu, Shihai, Sun, Junfeng, Lan, Qing. 2013. TGF-β-induced miR10a/b expression promotes human glioma cell migration by targeting PTEN. In Molecular medicine reports, 8, 1741-6. doi:10.3892/mmr.2013.1709. https://pubmed.ncbi.nlm.nih.gov/24100613/
3. Das, Kaushik, Rao, L Vijaya Mohan. 2022. The Role of microRNAs in Inflammation. In International journal of molecular sciences, 23, . doi:10.3390/ijms232415479. https://pubmed.ncbi.nlm.nih.gov/36555120/
4. Collins, Kaitlyn E, Wang, Xiyin, Klymenko, Yuliya, Creighton, Chad J, Hawkins, Shannon M. 2023. Transcriptomic analyses of ovarian clear-cell carcinoma with concurrent endometriosis. In Frontiers in endocrinology, 14, 1162786. doi:10.3389/fendo.2023.1162786. https://pubmed.ncbi.nlm.nih.gov/37621654/
5. Neth, Peter, Nazari-Jahantigh, Maliheh, Schober, Andreas, Weber, Christian. 2013. MicroRNAs in flow-dependent vascular remodelling. In Cardiovascular research, 99, 294-303. doi:10.1093/cvr/cvt096. https://pubmed.ncbi.nlm.nih.gov/23612583/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen