C57BL/6JCya-Morrbidem1/Cya
Common Name:
Morrbid-KO
Product ID:
S-KO-20377
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Morrbid-KO
Strain ID
KOCMP-100043424-Morrbid-B6J-VA
Gene Name
Product ID
S-KO-20377
Gene Alias
Cytor; Gm14005; Mir4435-2hg
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Morrbidem1/Cya mice (Catalog S-KO-20377) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000225087
NCBI RefSeq
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Target Region
Exon 1~2
Size of Effective Region
~181.9 kb
Detailed Document
Overview of Gene Research
Morrbid, also known as Myeloid RNA regulator of Bim-induced death, is a long noncoding RNA (lncRNA) that has emerged as a significant regulator in multiple biological processes. It is involved in pathways related to apoptosis, cell differentiation, and splicing regulation, thereby playing a crucial role in maintaining normal cellular functions and influencing disease development [1,2,3,4,5,6,8]. Genetic models, such as knockout (KO) mouse models, have been instrumental in uncovering its functions.
In the context of acute myocardial infarction (AMI), Morrbid expression is increased in cardiomyocytes under hypoxia or oxidative stress and in mouse hearts with AMI. Overexpression of Morrbid reduces infarct size and cardiac dysfunction, while cardiomyocyte-specific Morrbid-KO (Morrbidfl/fl/Myh6-Cre) mice show deteriorated infarct size and cardiac function. This indicates that Morrbid protects hearts from AMI via anti-apoptosis through its target gene serpine1 [1].
In the case of monocyte-macrophage differentiation and atherogenesis, Morrbid expression increases during differentiation. Morrbid knockdown inhibits this differentiation and macrophage activity, and Morrbid-overexpressing mice show enhanced monocytes/macrophages recruitment and atherosclerotic lesion formation, while monocyte/macrophage-specific Morrbid knockout has the opposite effect, suggesting its role in atherogenesis [2].
In the context of leukemia, loss of Morrbid in mouse models of juvenile myelomonocytic leukemia (JMML) driven by the Shp2E76K/+ mutation and in models of hyperglycemia-induced leukemia corrects myeloid and erythroid cell abnormalities, prolongs survival, and mitigates the disease, indicating Morrbid's contribution to leukemia pathogenesis [3,7].
In conclusion, Morrbid is a key lncRNA that regulates apoptosis, cell differentiation, and splicing, influencing diseases such as AMI, atherosclerosis, and leukemia. The use of Morrbid KO/CKO mouse models has provided valuable insights into its role in these disease areas, highlighting its potential as a therapeutic target for ischemic heart diseases and myeloid neoplasms [1,2,3,7].
References：
1. Yu, Yang, Yang, Haiqiong, Li, Qiuting, Wang, Xiaobin, Zhang, Chunxiang. 2023. Stress-enhanced cardiac lncRNA Morrbid protects hearts from acute myocardial infarction. In JCI insight, 8, . doi:10.1172/jci.insight.165568. https://pubmed.ncbi.nlm.nih.gov/37432746/
2. Xiang, Di, Jiang, Lei, Yuan, Qiong, Qin, Gangjian, Zhang, Chunxiang. 2023. Leukocyte-Specific Morrbid Promotes Leukocyte Differentiation and Atherogenesis. In Research (Washington, D.C.), 6, 0187. doi:10.34133/research.0187. https://pubmed.ncbi.nlm.nih.gov/37426471/
3. Cai, Zhigang, Zhang, Chi, Kotzin, Jonathan J, Henao-Mejia, Jorge, Kapur, Reuben. . Role of lncRNA Morrbid in PTPN11(Shp2)E76K-driven juvenile myelomonocytic leukemia. In Blood advances, 4, 3246-3251. doi:10.1182/bloodadvances.2020002123. https://pubmed.ncbi.nlm.nih.gov/32697817/
4. Cai, Zhigang, Aguilera, Fabiola, Ramdas, Baskar, Henao-Mejia, Jorge, Kapur, Reuben. . Targeting Bim via a lncRNA Morrbid Regulates the Survival of Preleukemic and Leukemic Cells. In Cell reports, 31, 107816. doi:10.1016/j.celrep.2020.107816. https://pubmed.ncbi.nlm.nih.gov/32579941/
5. Kotzin, Jonathan J, Iseka, Fany, Wright, Jasmine, Wherry, E John, Henao-Mejia, Jorge. 2019. The long noncoding RNA Morrbid regulates CD8 T cells in response to viral infection. In Proceedings of the National Academy of Sciences of the United States of America, 116, 11916-11925. doi:10.1073/pnas.1819457116. https://pubmed.ncbi.nlm.nih.gov/31138702/
6. Fefilova, Anna, Melnikov, Pavel, Prikazchikova, Tatiana, Sergeeva, Olga, Zatsepin, Timofei S. 2020. Murine Long Noncoding RNA Morrbid Contributes in the Regulation of NRAS Splicing in Hepatocytes In Vitro. In International journal of molecular sciences, 21, . doi:10.3390/ijms21165605. https://pubmed.ncbi.nlm.nih.gov/32764370/
7. Cai, Zhigang, Lu, Xiaoyu, Zhang, Chi, Haneline, Laura, Kapur, Reuben. . Hyperglycemia cooperates with Tet2 heterozygosity to induce leukemia driven by proinflammatory cytokine-induced lncRNA Morrbid. In The Journal of clinical investigation, 131, . doi:10.1172/JCI140707. https://pubmed.ncbi.nlm.nih.gov/33090974/
8. Kotzin, Jonathan J, Spencer, Sean P, McCright, Sam J, Flavell, Richard A, Henao-Mejia, Jorge. 2016. The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan. In Nature, 537, 239-243. doi:10.1038/nature19346. https://pubmed.ncbi.nlm.nih.gov/27525555/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen