C57BL/6JCya-Map2k6em1/Cya
Common Name:
Map2k6-KO
Product ID:
S-KO-20380
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Map2k6-KO
Strain ID
KOCMP-26399-Map2k6-B6J-VB
Gene Name
Product ID
S-KO-20380
Gene Alias
MAPKK 6; MAPKK6; MEK6; MKK6; Prkmk6; SAPKK3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Map2k6em1/Cya mice (Catalog S-KO-20380) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020949
NCBI RefSeq
NM_011943
Target Region
Exon 3~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Map2k6, also known as Mitogen-activated protein kinase kinase 6 (Mkk6), is a key component in the mitogen-activated protein kinase (MAPK) signaling pathway. It is involved in regulating various biological processes such as chromatin remodeling, cell proliferation, apoptosis, and stress responses. Dysregulation of Map2k6 has been linked to several diseases, highlighting its biological importance [1-10].
In somatic cell reprogramming, Map2k6 acts as a chromatin relaxer, enhancing the process. Its function in heterochromatin loosening and reprogramming requires kinase activity but is independent of its well-known target P38. It phosphorylates Gatad2b, which in turn elevates histone acetylation levels and loosens heterochromatin, promoting pluripotency gene expression [1].
In polycystic ovary syndrome (PCOS), circASPH promotes KGN cells proliferation through the miR-375/MAP2K6 axis, and circ_0005925 promotes granulosa cell growth by targeting miR-324-3p to upregulate MAP2K6, suggesting its role in PCOS-related cell growth regulation [2,6].
In ovarian cancer, MAP2K6-FP enhances the sensitiveness of paclitaxel via inducing autophagy [3].
In nasopharyngeal carcinoma, high MAP2K6 expression is associated with radiation resistance and poor prognosis [4].
In LPS-induced C28/I2 chondrocyte injury, circRNA-MSR regulates the process through the miR-643/MAP2K6 signaling pathway [5].
In hepatocellular carcinoma, APEX1 promotes oncogenicity via regulation of MAP2K6 [7].
In gut stem cell aging, mTORC1 increases MKK6 protein synthesis, activating the p38 MAPK-p53 pathway, leading to stem cell and villus aging [8].
In cell senescence, lncRNA MAGI2-AS3 affects the process via the ROS/MAP2K6/p38 signaling pathway [9].
In conclusion, Map2k6 plays essential roles in diverse biological processes and is closely associated with multiple diseases. Functional studies, especially those using in vivo models like KO/CKO mouse models (although not explicitly mentioned in the provided abstracts but commonly used in such research), could further clarify its detailed mechanisms in these biological processes and disease conditions, providing potential therapeutic targets for related diseases.
References：
1. Xing, Guangsuo, Liu, Zichao, Huang, Luyuan, Liu, Xingguo, Chen, Keshi. 2021. MAP2K6 remodels chromatin and facilitates reprogramming by activating Gatad2b-phosphorylation dependent heterochromatin loosening. In Cell death and differentiation, 29, 1042-1054. doi:10.1038/s41418-021-00902-z. https://pubmed.ncbi.nlm.nih.gov/34815549/
2. Wu, Gengxiang, Xia, Jing, Yang, Zhe, Yin, Tailang, Yang, Jing. 2020. CircASPH promotes KGN cells proliferation through miR-375/MAP2K6 axis in Polycystic Ovary Syndrome. In Journal of cellular and molecular medicine, 26, 1817-1825. doi:10.1111/jcmm.16231. https://pubmed.ncbi.nlm.nih.gov/33372369/
3. Guo, Ying, Yuan, Jin, Yin, Shuqin, Shuai, Rong, Kang, Jiali. . MAP2K6-FP Enhances the Sensitiveness of Paclitaxel for Ovarian Cancer via Inducing Autophagy. In International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 27, 1082-1087. doi:10.1097/IGC.0000000000001003. https://pubmed.ncbi.nlm.nih.gov/28604448/
4. Li, Zhanzhan, Li, Na, Shen, Liangfang. 2018. MAP2K6 is associated with radiation resistance and adverse prognosis for locally advanced nasopharyngeal carcinoma patients. In Cancer management and research, 10, 6905-6912. doi:10.2147/CMAR.S184689. https://pubmed.ncbi.nlm.nih.gov/30588096/
5. Jia, Zhen, Wei, Qing-Jun. 2021. CircRNA-MSR Regulates LPS-Induced C28/I2 Chondrocyte Injury through miR-643/MAP2K6 Signaling Pathway. In Cartilage, 13, 785S-795S. doi:10.1177/19476035211044826. https://pubmed.ncbi.nlm.nih.gov/34581623/
6. Tu, Peng, Yan, Sujuan, Zhang, Fengping. 2022. Circ_0005925 Promotes Granulosa Cell Growth by Targeting MiR-324-3p to Upregulate MAP2K6 in Polycystic Ovary Syndrome. In Biochemical genetics, 61, 21-34. doi:10.1007/s10528-022-10238-z. https://pubmed.ncbi.nlm.nih.gov/35689709/
7. Sun, Zhipeng, Chen, Guangyang, Wang, Liang, Xu, Guangzhong, Zhang, Nengwei. 2022. APEX1 promotes the oncogenicity of hepatocellular carcinoma via regulation of MAP2K6. In Aging, 14, 7959-7971. doi:10.18632/aging.204325. https://pubmed.ncbi.nlm.nih.gov/36205565/
8. He, Dan, Wu, Hongguang, Xiang, Jinnan, Liu, Huijuan, Li, Baojie. 2020. Gut stem cell aging is driven by mTORC1 via a p38 MAPK-p53 pathway. In Nature communications, 11, 37. doi:10.1038/s41467-019-13911-x. https://pubmed.ncbi.nlm.nih.gov/31896747/
9. Zhang, Yingmin, Qiao, Xinhua, Liu, Lihui, Chen, Runsheng, Chen, Chang. 2022. Long noncoding RNA MAGI2-AS3 regulates the H2O2 level and cell senescence via HSPA8. In Redox biology, 54, 102383. doi:10.1016/j.redox.2022.102383. https://pubmed.ncbi.nlm.nih.gov/35797800/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen