C57BL/6JCya-Dact2em1/Cya
Common Name:
Dact2-KO
Product ID:
S-KO-20391
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dact2-KO
Strain ID
KOCMP-240025-Dact2-B6J-VB
Gene Name
Product ID
S-KO-20391
Gene Alias
2900084M21Rik; A630024E20; Dpr2; Frd2; dapper2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dact2em1/Cya mice (Catalog S-KO-20391) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053218
NCBI RefSeq
NM_172826
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Dact2, also known as Dapper homolog 2, is a member of the DACT gene family. It plays crucial roles in tissue development and injury, and is involved in regulating intracellular signaling pathways, especially the Wnt/β -catenin and TGF -β signaling pathways. These pathways are fundamental in various biological processes, and Dact2's regulation of them is of great biological importance. Genetic models, such as zebrafish and mouse models, have been used to study Dact2 [1,2,3].
In a zebrafish Dact2 knockout model, the absence of Dact2 enhanced the expression of genes related to tumor invasion, migration, and epithelial-mesenchymal transition (EMT), resulting in hyperplasia of the gastrointestinal epithelium, fibrosis in the pancreas and liver, and increased proliferation of the pancreatic and hepatic bile ducts [2]. In mice, knockout of Dact2 alleviated cisplatin-induced nephrotoxicity. RNA sequencing revealed differences in gene expression related to cell proliferation, apoptosis, and the MAPK pathway between wild-type and Dact2 knockout mice. β-catenin was found to directly bind to and regulate the transcription of Igf1, which was involved in the alleviation of cisplatin-induced cytotoxicity [3].
In conclusion, Dact2 is an important regulator of key signaling pathways. Gene knockout models in zebrafish and mice have revealed its role in processes like EMT, tissue fibrosis, and drug-induced nephrotoxicity. Understanding Dact2's function can provide insights into the mechanisms of these biological processes and disease conditions, potentially guiding the development of new treatment strategies.
References:
1. Lai, Xiaofan, Huang, Shaojie, Lin, Yingying, Huang, Wenqi, Wang, Zhongxing. 2022. DACT2 protects against pulmonary fibrosis via suppressing glycolysis in lung myofibroblasts. In International journal of biological macromolecules, 226, 291-300. doi:10.1016/j.ijbiomac.2022.11.324. https://pubmed.ncbi.nlm.nih.gov/36481337/
2. Kim, Dong Hee, Kim, Eun Ji, Kim, Do Hee, Park, Seung Woo. 2020. Dact2 is involved in the regulation of epithelial-mesenchymal transition. In Biochemical and biophysical research communications, 524, 190-197. doi:10.1016/j.bbrc.2019.12.090. https://pubmed.ncbi.nlm.nih.gov/31983425/
3. Kim, Changuk, Kwak, Woori, Won, Dong-Hoon, Park, Jun Won, Yun, Jun-Won. 2023. Loss of Dact2 alleviates cisplatin-induced nephrotoxicity through regulation of the Igfl-MAPK pathway axis. In Cell biology and toxicology, 39, 3197-3217. doi:10.1007/s10565-023-09827-4. https://pubmed.ncbi.nlm.nih.gov/37603122/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen