C57BL/6JCya-Txlnbem1/Cya
Common Name:
Txlnb-KO
Product ID:
S-KO-20400
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Txlnb-KO
Strain ID
KOCMP-378431-Txlnb-B6J-VB
Gene Name
Product ID
S-KO-20400
Gene Alias
2310001N14Rik; Mdp77
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Txlnbem1/Cya mice (Catalog S-KO-20400) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037964
NCBI RefSeq
NM_138628
Target Region
Exon 3
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Txlnb, also known as β -taxilin, is a cardiomyocyte-enriched protein belonging to a family of centrosome adapter proteins implicated in protein quality control. It is involved in cytoskeletal, microtubule, and the ubiquitin-proteasome system (UPS) processes, especially those related to centrosome functions. The centrosome-proteasome axis, in which Txlnb may play a bridging role, is potentially important for proteostasis in cardiomyocytes [1,2].
In Txlnb-knockout (KO) mouse models, there is proteasomal insufficiency and altered cardiac growth. Ubiquitinated protein accumulates, 26Sβ5 proteasome activity decreases, and cardiac mass lowers with age. In models of cardiac proteotoxicity (CryAB-R120G mice) and pressure overload (transverse aortic constriction, TAC), loss of Txlnb worsens heart phenotypes, such as reduced ejection fraction and increased heart mass [1,2]. In C2C12 cells, knockdown of β -taxilin (Txlnb) impairs the fusion of myoblasts into myotubes and decreases myotube diameter, indicating its role in myogenesis [3].
In conclusion, Txlnb plays a crucial role in cardiac proteostasis and myogenesis. The Txlnb KO mouse models and cell-based knockdown experiments have revealed its significance in cardiac-related diseases and muscle development, highlighting the importance of the centrosome-proteasome connection in cardiomyocytes and the role of Txlnb in myoblast differentiation.
References:
1. McLendon, Jared M, Zhang, Xiaoming, Stein, Colleen S, Bodine, Sue C, Boudreau, Ryan L. 2024. A Specialized Centrosome-Proteasome Axis Mediates Proteostasis and Influences Cardiac Stress through Txlnb. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.02.12.580020. https://pubmed.ncbi.nlm.nih.gov/38405715/
2. McLendon, Jared M, Zhang, Xiaoming, Stein, Colleen S, Bodine, Sue C, Boudreau, Ryan L. 2025. Gain and loss of the centrosomal protein taxilin-beta influences cardiac proteostasis and stress. In Journal of molecular and cellular cardiology, 201, 56-69. doi:10.1016/j.yjmcc.2025.02.008. https://pubmed.ncbi.nlm.nih.gov/40010430/
3. Sakane, Hiroshi, Makiyama, Tomohiko, Nogami, Satoru, Akasaki, Kenji, Shirataki, Hiromichi. 2016. β-Taxilin participates in differentiation of C2C12 myoblasts into myotubes. In Experimental cell research, 345, 230-8. doi:10.1016/j.yexcr.2016.05.016. https://pubmed.ncbi.nlm.nih.gov/27231216/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen