C57BL/6JCya-Fmo1em1/Cya
Common Name:
Fmo1-KO
Product ID:
S-KO-20426
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fmo1-KO
Strain ID
KOCMP-14261-Fmo1-B6J-VA
Gene Name
Product ID
S-KO-20426
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fmo1em1/Cya mice (Catalog S-KO-20426) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046049
NCBI RefSeq
NM_010231
Target Region
Exon 3~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Fmo1, flavin containing dimethylaniline monooxygenase 1, is involved in lipid metabolism [2]. It also has a role in detoxifying xenobiotics and is part of the oxidative flavin-containing monooxygenases family [5]. In humans, its expression shows developmental and tissue-specific patterns, being restricted to the fetus in the liver while having extrahepatic expression in adult life [6,7].
In human skin, Fmo1 defines one of the major fibroblast populations, with Fmo1+ fibroblasts being larger and distributed in interstitial and perivascular locations, suggesting a role in matrix deposition, inflammatory cell retention, and connective tissue cell differentiation [1]. In non-alcoholic fatty liver disease (NAFLD), Fmo1 promotes disease progression by regulating PPARα activation and inducing ferroptosis. Knockdown of Fmo1 in an in vitro NAFLD model suppressed lipid accumulation, down-regulation of PPARα expression, and up-regulation of ferroptosis [2]. In classical papillary thyroid cancer, high Fmo1 expression independently predicts favorable recurrence-free survival [3]. In plants, Arabidopsis thaliana Fmo1 is involved in excess light stress-induced signal transduction and cell death signaling, playing a role in systemic acquired acclimation [4].
In conclusion, Fmo1 has diverse functions in different organisms and tissues. In mammals, it is crucial in lipid metabolism, fibroblast-related functions, and has implications in diseases like NAFLD and classical papillary thyroid cancer. Its role in plants in stress-related signaling further showcases its broad biological importance. The studies, including those using in vitro models for NAFLD, contribute to understanding the role of Fmo1 in specific biological processes and disease conditions.
References:
1. Tabib, Tracy, Morse, Christina, Wang, Ting, Chen, Wei, Lafyatis, Robert. 2017. SFRP2/DPP4 and FMO1/LSP1 Define Major Fibroblast Populations in Human Skin. In The Journal of investigative dermatology, 138, 802-810. doi:10.1016/j.jid.2017.09.045. https://pubmed.ncbi.nlm.nih.gov/29080679/
2. Zou, Lin, Shi, Qin, Li, Yingxuan, Zhu, Hongling, Ma, Junhua. . FMO1 Promotes Nonalcoholic Fatty Liver Disease Progression by Regulating PPARα Activation and Inducing Ferroptosis. In Discovery medicine, 35, 612-622. doi:10.24976/Discov.Med.202335177.60. https://pubmed.ncbi.nlm.nih.gov/37553313/
3. Luo, Jing, Zhang, Bowei, Cui, Likun, Liu, Tong, Gu, Yi. 2019. FMO1 gene expression independently predicts favorable recurrence-free survival of classical papillary thyroid cancer. In Future oncology (London, England), 15, 1303-1311. doi:10.2217/fon-2018-0885. https://pubmed.ncbi.nlm.nih.gov/30757917/
4. Czarnocka, Weronika, Fichman, Yosef, Bernacki, Maciej, Mittler, Ron, Karpiński, Stanisław. 2020. FMO1 Is Involved in Excess Light Stress-Induced Signal Transduction and Cell Death Signaling. In Cells, 9, . doi:10.3390/cells9102163. https://pubmed.ncbi.nlm.nih.gov/32987853/
5. Bailleul, Gautier, Nicoll, Callum R, Mascotti, María Laura, Mattevi, Andrea, Fraaije, Marco W. 2020. Ancestral reconstruction of mammalian FMO1 enables structural determination, revealing unique features that explain its catalytic properties. In The Journal of biological chemistry, 296, 100221. doi:10.1074/jbc.RA120.016297. https://pubmed.ncbi.nlm.nih.gov/33759784/
6. Koukouritaki, Sevasti B, Simpson, Pippa, Yeung, Catherine K, Rettie, Allan E, Hines, Ronald N. . Human hepatic flavin-containing monooxygenases 1 (FMO1) and 3 (FMO3) developmental expression. In Pediatric research, 51, 236-43. doi:. https://pubmed.ncbi.nlm.nih.gov/11809920/
7. Yeung, C K, Lang, D H, Thummel, K E, Rettie, A E. . Immunoquantitation of FMO1 in human liver, kidney, and intestine. In Drug metabolism and disposition: the biological fate of chemicals, 28, 1107-11. doi:. https://pubmed.ncbi.nlm.nih.gov/10950857/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen