C57BL/6JCya-Rradem1/Cya
Common Name:
Rrad-KO
Product ID:
S-KO-20605
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Rrad-KO
Strain ID
KOCMP-56437-Rrad-B6J-VC
Gene Name
Product ID
S-KO-20605
Gene Alias
REM3; Rad
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rradem1/Cya mice (Catalog S-KO-20605) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034351
NCBI RefSeq
NM_019662
Target Region
Exon 3~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
RRAD, also known as Ras-related associated with diabetes, is a member of the Ras-related GTPase superfamily. Primarily a cytosolic protein that acts in the plasma membrane, it is highly expressed in type 2 diabetes patients and serves as a biomarker for congestive heart failure. RRAD is involved in multiple cellular activities including cell division, motility, apoptosis, and energy metabolism, and is regulated through various pathways [1].
RRAD shows dual roles in cancer. In gastric and colorectal cancer, its inhibition significantly declined tumor cell proliferation in vitro and in vivo, reduced cell invasion, decreased EMT markers, angiogenesis, and associated proteins levels, suggesting it as a potential therapeutic target [2]. In hepatocellular carcinoma, RRAD suppresses the Warburg effect by downregulating ACTG1, retarding tumor growth, and is associated with better prognosis [3]. In contrast, in glioblastoma multiforme, RRAD promotes EGFR-mediated STAT3 activation, enhancing self-renewing ability, tumorigenesis, and inducing temozolomide resistance [4]. In pancreatic cancer, SETD8 inhibits ferroptosis by suppressing RRAD expression, and in oral squamous cell carcinoma, downregulation of RRAD in the tumor margin promotes energy metabolism and tumor progression [5,6]. Also, RRAD is up-regulated in cellular senescence and acts as a negative regulator to counter it by reducing reactive oxygen species levels [7].
In conclusion, RRAD is a multifunctional gene involved in diverse biological processes, especially in energy metabolism and cell growth regulation. Its dual role in cancer, as both an oncogene and a tumor suppressor depending on the cancer type, makes it a potential target for cancer therapies. Additionally, its role in senescence further highlights its importance in understanding aging-associated diseases.
References:
1. Sun, Zhangyue, Li, Yongkang, Tan, Xiaolu, Xu, Liyan, Long, Lin. 2023. Friend or Foe: Regulation, Downstream Effectors of RRAD in Cancer. In Biomolecules, 13, . doi:10.3390/biom13030477. https://pubmed.ncbi.nlm.nih.gov/36979412/
2. Kim, Hee Kyung, Lee, Inkyoung, Kim, Seung Tae, Park, Joon Oh, Kang, Won Ki. 2019. RRAD expression in gastric and colorectal cancer with peritoneal carcinomatosis. In Scientific reports, 9, 19439. doi:10.1038/s41598-019-55767-7. https://pubmed.ncbi.nlm.nih.gov/31857616/
3. Yan, Yingcai, Xu, Hao, Zhang, Linshi, Ge, Wenhao, Wang, Weilin. 2019. RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinoma. In OncoTargets and therapy, 12, 1691-1703. doi:10.2147/OTT.S197844. https://pubmed.ncbi.nlm.nih.gov/30881024/
4. Yeom, Seon-Yong, Nam, Do-Hyun, Park, Chaehwa. 2014. RRAD promotes EGFR-mediated STAT3 activation and induces temozolomide resistance of malignant glioblastoma. In Molecular cancer therapeutics, 13, 3049-61. doi:10.1158/1535-7163.MCT-14-0244. https://pubmed.ncbi.nlm.nih.gov/25313011/
5. Lu, Zekun, Hu, Qiangsheng, Qin, Yi, Xu, Xiaowu, Chen, Xuemin. 2023. SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRAD. In Cancer cell international, 23, 50. doi:10.1186/s12935-023-02899-6. https://pubmed.ncbi.nlm.nih.gov/36934248/
6. Cheng, Aoming, Xu, Qiaoshi, Li, Bo, Han, Zhengxue, Feng, Zhien. 2024. The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma. In Cell death & disease, 15, 376. doi:10.1038/s41419-024-06759-7. https://pubmed.ncbi.nlm.nih.gov/38811531/
7. Wei, Zhao, Guo, Haiyang, Qin, Junchao, Gong, Yaoqin, Shao, Changshun. 2018. Pan-senescence transcriptome analysis identified RRAD as a marker and negative regulator of cellular senescence. In Free radical biology & medicine, 130, 267-277. doi:10.1016/j.freeradbiomed.2018.10.457. https://pubmed.ncbi.nlm.nih.gov/30391675/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen