C57BL/6JCya-Iws1em1/Cya
Common Name:
Iws1-KO
Product ID:
S-KO-20809
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Iws1-KO
Strain ID
KOCMP-73473-Iws1-B6J-VB
Gene Name
Product ID
S-KO-20809
Gene Alias
1700069O15Rik; 3000008H23
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Iws1em1/Cya mice (Catalog S-KO-20809) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025243
NCBI RefSeq
NM_173441
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Iws1, an RNA-polymerase II (RNAPII)-associated transcription elongation factor, is conserved from yeast (where it is known as Spn1) to humans. It is essential for various biological processes. Iws1 interacts with the histone H3/H4 chaperone Spt6, recruits the lysine methyltransferase Setd2 to chromatin, and is involved in regulating H3K36me3, which is crucial for pre-mRNA splicing and mRNA export. Genetic models, like KO mouse models, are valuable for studying its functions [2,4,5,6].
In Toxoplasma gondii, IWS1-deficient parasites show decreased ROP18 mRNA expression, increased loading of IFN-inducible GTPases onto the parasitophorous vacuole membrane, and reduced virulence in wild-type mice but normal virulence in IFN-γ receptor-lacking mice. This indicates that T. gondii IWS1 indirectly regulates ROP18 mRNA expression to determine fitness in IFN-γ-activated host cells and mice [1].
In mice, knockdown of either Iws1 or Supt6 (Spt6 in mouse gene ontology) blocks embryo development, primarily at the 8/16-cell stage, and affects pre-mRNA splicing, mRNA export, and the expression of lineage-specific transcription factors. Iws1 is also required for H3K36 trimethylation during mouse early development [2].
In liposarcoma, AKT-mediated phosphorylation of IWS1 promotes the maintenance of cancer stem cells, contributes to a "metastable" cell phenotype, and is associated with reduced overall survival in patients [3].
In summary, Iws1 plays essential roles in multiple biological processes such as parasite survival in host cells, embryonic development, and cancer cell biology. The study of Iws1 through KO/CKO mouse models has provided insights into its functions in these specific disease areas, including parasite-host interactions, embryonic development, and liposarcoma [1,2,3].
References:
1. Hashizaki, Emi, Sasai, Miwa, Okuzaki, Daisuke, Iwanaga, Shiroh, Yamamoto, Masahiro. 2023. Toxoplasma IWS1 Determines Fitness in Interferon-γ-Activated Host Cells and Mice by Indirectly Regulating ROP18 mRNA Expression. In mBio, 14, e0325622. doi:10.1128/mbio.03256-22. https://pubmed.ncbi.nlm.nih.gov/36715543/
2. Oqani, Reza K, Lin, Tao, Lee, Jae Eun, Shin, Hyun Young, Il Jin, Dong. 2019. Iws1 and Spt6 Regulate Trimethylation of Histone H3 on Lysine 36 through Akt Signaling and are Essential for Mouse Embryonic Genome Activation. In Scientific reports, 9, 3831. doi:10.1038/s41598-019-40358-3. https://pubmed.ncbi.nlm.nih.gov/30846735/
3. Wang, Yu, Zhang, Hongji, La Ferlita, Alessandro, Pollock, Raphael E, Beane, Joal D. 2023. Phosphorylation of IWS1 by AKT maintains liposarcoma tumor heterogeneity through preservation of cancer stem cell phenotypes and mesenchymal-epithelial plasticity. In Oncogenesis, 12, 30. doi:10.1038/s41389-023-00469-z. https://pubmed.ncbi.nlm.nih.gov/37237004/
4. Yoh, Sunnie M, Lucas, Joseph S, Jones, Katherine A. . The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation. In Genes & development, 22, 3422-34. doi:10.1101/gad.1720008. https://pubmed.ncbi.nlm.nih.gov/19141475/
5. Orlacchio, Arturo, Stark, Aaron E, Foray, Claudia, Tsichlis, Philip N, Coppola, Vincenzo. 2018. Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality. In PloS one, 13, e0201030. doi:10.1371/journal.pone.0201030. https://pubmed.ncbi.nlm.nih.gov/30208029/
6. López-Rivera, Francheska, Chuang, James, Spatt, Dan, Gopalakrishnan, Rajaraman, Winston, Fred. . Suppressor mutations that make the essential transcription factor Spn1/Iws1 dispensable in Saccharomyces cerevisiae. In Genetics, 222, . doi:10.1093/genetics/iyac125. https://pubmed.ncbi.nlm.nih.gov/35977387/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen