C57BL/6JCya-Yrdcem1/Cya
Common Name:
Yrdc-KO
Product ID:
S-KO-20849
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Yrdc-KO
Strain ID
KOCMP-230734-Yrdc-B6J-VB
Gene Name
Product ID
S-KO-20849
Gene Alias
IRIP; ITIP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Yrdcem1/Cya mice (Catalog S-KO-20849) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102628
NCBI RefSeq
NM_153566
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
YRDC, or yrdC N6 -threonylcarbamoltransferase domain containing protein, is an essential transfer RNA (tRNA) modification enzyme. It catalyzes the formation of N6 -threonylcarbamoyladenosine (t6A) on ANN-decoding tRNA species, which is crucial for accurate protein synthesis and is associated with pathways like translation [1,5,7,9,10]. It also interacts with the KEOPS complex, playing a role in maintaining genome integrity and telomere homeostasis [5]. Genetic models can be used to study its functions in biological processes and diseases.
Knockdown of YRDC in glioblastoma stem cells reduced t6A formation, suppressed global translation and inhibited tumor growth both in vitro and in vivo. Threonine, an essential substrate of YRDC, accumulated in these cells, facilitating t6A formation and shifting the proteome to support mitosis-related genes [1]. In hepatocarcinoma cells, YRDC knockdown decreased susceptibility to lenvatinib, and it was found to regulate the protein translation of KRAS [2]. In non-small cell lung cancer (NSCLC), knockdown of YRDC in cell lines suppressed cell growth and colony formation and induced apoptosis [3]. In hepatocellular carcinoma, YRDC depletion suppressed proliferation, migration and invasion of cells, while overexpression had the opposite effect, and it promoted progression by activating MEK/ERK signaling pathways [4]. Biallelic variants in YRDC in a patient caused a developmental disorder with progeroid features, due to impaired YRDC function, reduced t6A modifications, telomere shortening and DNA repair defects [5]. In NSCLC cells, RNA structure formation in YRDC 3' UTR can suppress ELAVL1 binding, leading to EGFR-TKI sensitivity by impairing YRDC translation [6]. In osteosarcoma, knockdown of circRBMS3, which can regulate YRDC through 'sponging' miR-424-5p, inhibited malignant phenotypes in vivo [8]. Mutations in YRDC also cause an extremely severe form of Galloway-Mowat syndrome [9].
In conclusion, YRDC is crucial for tRNA modification and accurate protein synthesis. Model-based research, especially through loss-of-function experiments, has revealed its significant roles in multiple diseases such as various cancers, developmental disorders, and Galloway-Mowat syndrome. Understanding YRDC can provide insights into disease mechanisms and potential therapeutic targets.
References:
1. Wu, Xujia, Yuan, Huairui, Wu, Qiulian, Zhang, Nu, Rich, Jeremy N. 2024. Threonine fuels glioblastoma through YRDC-mediated codon-biased translational reprogramming. In Nature cancer, 5, 1024-1044. doi:10.1038/s43018-024-00748-7. https://pubmed.ncbi.nlm.nih.gov/38519786/
2. Guo, Jun, Zhu, Peng, Ye, Zhi, Zhou, Honghao, Li, Qing. 2021. YRDC Mediates the Resistance of Lenvatinib in Hepatocarcinoma Cells via Modulating the Translation of KRAS. In Frontiers in pharmacology, 12, 744578. doi:10.3389/fphar.2021.744578. https://pubmed.ncbi.nlm.nih.gov/34658879/
3. Shen, Haibo, Zheng, Enkuo, Yang, Zhenhua, Li, Rui, Zhao, Guofang. 2020. YRDC is upregulated in non-small cell lung cancer and promotes cell proliferation by decreasing cell apoptosis. In Oncology letters, 20, 43-52. doi:10.3892/ol.2020.11560. https://pubmed.ncbi.nlm.nih.gov/32565932/
4. Huang, Shiqiong, Zhu, Peng, Sun, Bao, Shu, Yan, Li, Qing. 2019. Modulation of YrdC promotes hepatocellular carcinoma progression via MEK/ERK signaling pathway. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 114, 108859. doi:10.1016/j.biopha.2019.108859. https://pubmed.ncbi.nlm.nih.gov/30978526/
5. Schmidt, Julia, Goergens, Jonas, Pochechueva, Tatiana, Yigit, Gökhan, Wollnik, Bernd. 2021. Biallelic variants in YRDC cause a developmental disorder with progeroid features. In Human genetics, 140, 1679-1693. doi:10.1007/s00439-021-02347-3. https://pubmed.ncbi.nlm.nih.gov/34545459/
6. Shi, Boyang, An, Ke, Wang, Yueqin, Tian, Xin, Kan, Quancheng. 2022. RNA Structural Dynamics Modulate EGFR-TKI Resistance Through Controlling YRDC Translation in NSCLC Cells. In Genomics, proteomics & bioinformatics, 21, 850-865. doi:10.1016/j.gpb.2022.10.006. https://pubmed.ncbi.nlm.nih.gov/36435452/
7. Harris, Kimberly A, Jones, Victoria, Bilbille, Yann, Swairjo, Manal A, Agris, Paul F. 2011. YrdC exhibits properties expected of a subunit for a tRNA threonylcarbamoyl transferase. In RNA (New York, N.Y.), 17, 1678-87. doi:10.1261/rna.2592411. https://pubmed.ncbi.nlm.nih.gov/21775474/
8. Gong, Zhe, Shen, Panyang, Wang, Haitao, Fang, Xiangqian, Liu, Gang. 2023. A novel circular RNA circRBMS3 regulates proliferation and metastasis of osteosarcoma by targeting miR-424-eIF4B/YRDC axis. In Aging, 15, 1564-1590. doi:10.18632/aging.204567. https://pubmed.ncbi.nlm.nih.gov/36897170/
9. Arrondel, Christelle, Missoury, Sophia, Snoek, Rozemarijn, van Tilbeurgh, Herman, Mollet, Géraldine. 2019. Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. In Nature communications, 10, 3967. doi:10.1038/s41467-019-11951-x. https://pubmed.ncbi.nlm.nih.gov/31481669/
10. El Yacoubi, Basma, Lyons, Benjamin, Cruz, Yulien, Swairjo, Manal A, de Crécy-Lagard, Valérie. 2009. The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA. In Nucleic acids research, 37, 2894-909. doi:10.1093/nar/gkp152. https://pubmed.ncbi.nlm.nih.gov/19287007/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen