C57BL/6JCya-Gzmaem1/Cya
Common Name:
Gzma-KO
Product ID:
S-KO-20851
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gzma-KO
Strain ID
KOCMP-14938-Gzma-B6J-VB
Gene Name
Product ID
S-KO-20851
Gene Alias
Ctla-3; Ctla3; Hf; Hf1; SE1; TSP-1; TSP1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gzmaem1/Cya mice (Catalog S-KO-20851) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023897
NCBI RefSeq
NM_010370
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
GZMA, also known as granzyme A, is a serine protease. It is involved in cytotoxic lymphocyte-mediated immunity and is thought to play a role in various cell death-related processes and immune-related pathways [2]. It has significance in maintaining normal physiological functions and is closely associated with multiple disease-related biological processes.
In inflammatory bowel disease (IBD), GZMA was found to suppress GPX4-mediated ferroptosis, promoting intestinal epithelial barrier function by enhancing CDX2-mediated cell differentiation, which induced the expression of Occludin and Zonula Occludens-1. Administration of GZMA could alleviate DSS-induced colitis in vivo [1].
In chronic apical periodontitis, inhibition of GZMA using crRNA/Cas13d led to decreased osteoclast cell proliferation, increased apoptosis, and changes in miR-25-3p, TGF-β, and PAR1 expression within the PARs pathway, suggesting GZMA as a potential therapeutic target [3].
In hepatocellular carcinoma, GZMA secreted by cytotoxic cells interacted with F2R expressed by tumor cells, activating the JAK2/STAT1 signaling pathway to induce tumor suppression, and low GZMA and F2R expression in tumor tissues was associated with poor prognosis [4].
In summary, GZMA has diverse functions in different biological processes and disease conditions. Its role in promoting intestinal epithelial barrier function in IBD, regulating osteoclast function in chronic apical periodontitis, and affecting tumor suppression in hepatocellular carcinoma, among others, highlights its importance. The use of genetic models such as those in the above-mentioned studies helps to uncover these functions, providing potential therapeutic directions for related diseases.
References：
1. Niu, Rongwei, Lan, Jiaoli, Liang, Danxia, Gong, Sitang, Yang, Min. 2024. GZMA suppressed GPX4-mediated ferroptosis to improve intestinal mucosal barrier function in inflammatory bowel disease. In Cell communication and signaling : CCS, 22, 474. doi:10.1186/s12964-024-01836-y. https://pubmed.ncbi.nlm.nih.gov/39367435/
2. Zhou, Zhiwei, He, Huabin, Wang, Kun, Ding, Jingjin, Shao, Feng. 2020. Granzyme A from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells. In Science (New York, N.Y.), 368, . doi:10.1126/science.aaz7548. https://pubmed.ncbi.nlm.nih.gov/32299851/
3. Jia, Tingting, Yuan, Fang, Tao, Jingqiao, Zhang, Bin, Li, Hongbo. 2023. CRISPR/Cas13d targeting GZMA in PARs pathway regulates the function of osteoclasts in chronic apical periodontitis. In Cellular & molecular biology letters, 28, 70. doi:10.1186/s11658-023-00477-2. https://pubmed.ncbi.nlm.nih.gov/37626297/
4. Gao, Yuxue, Xu, Qingguo, Li, Xinqiang, Chen, Dexi, Yang, Tongwang. 2022. Heterogeneity induced GZMA-F2R communication inefficient impairs antitumor immunotherapy of PD-1 mAb through JAK2/STAT1 signal suppression in hepatocellular carcinoma. In Cell death & disease, 13, 213. doi:10.1038/s41419-022-04654-7. https://pubmed.ncbi.nlm.nih.gov/35256589/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen