C57BL/6JCya-Clic3em1/Cya
Common Name
Clic3-KO
Product ID
S-KO-20855
Backgroud
C57BL/6JCya
Strain ID
KOCMP-69454-Clic3-B6J-VB
When using this mouse strain in a publication, please cite “Clic3-KO Mouse (Catalog S-KO-20855) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clic3-KO
Strain ID
KOCMP-69454-Clic3-B6J-VB
Gene Name
Product ID
S-KO-20855
Gene Alias
2300003G24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000114265
NCBI RefSeq
NM_027085
Target Region
Exon 2~6
Size of Effective Region
~1.6 kb
Overview of Gene Research
CLIC3, chloride intracellular channel 3, belongs to the glutathione-S-transferase (GSTs) superfamily. It regulates fundamental cellular processes like chloride ion concentration regulation, cell membrane potential stabilization, and is involved in trans-epithelial transport, cell volume regulation, and apoptotic processes [4].
In cancer research, CLIC3 has been found to be significantly involved. In bladder cancer, it promotes cell proliferation by reducing p21 expression through interacting with NAT10 and inhibiting N4-acetylcytidine modification of p21 mRNA, indicating its potential as a therapeutic target [1]. In breast cancer, CLIC3 controls the recycling of MT1-MMP from late endosomes to sites of cell-matrix adhesion, and its knockdown opposes MT1-MMP-dependent invasive processes, predicting poor prognosis in ER-negative breast cancer [3]. In pancreatic ductal adenocarcinoma, CLIC3, collaborating with Rab25, promotes integrin recycling from late endosomes/lysosomes, and its expression predicts lymph node metastasis and poor prognosis [7]. In gastric cancer, CLIC3 functions as a Cl-channel in the plasma membrane, and decreased expression is associated with unfavorable prognosis [2]. In salivary gland mucoepidermoid carcinoma, CLIC3 promoter hypomethylation and overexpression are significant events [8].
In schizophrenia research, CLIC3 was identified as an immune-related hub gene, potentially a diagnostic biomarker and therapeutic target, as its levels were reduced in a schizophrenia rat model and reversed by an antipsychotic drug [5]. Also, in fibroblast cellular senescence, knockdown of CLIC3 mitigated various senescence-related phenotypes by interacting with ERK7 [6].
In conclusion, CLIC3 is involved in multiple biological processes and disease conditions. Its roles in cancer progression, including bladder, breast, pancreatic, gastric, and salivary gland cancers, are well-studied. In addition, it has potential implications in schizophrenia and fibroblast cellular senescence. Research on CLIC3 using gene-knockout or other loss-of-function models has provided valuable insights into its functions in these disease areas, helping to understand the underlying molecular mechanisms and potentially guiding new therapeutic strategies.
References：
1. Shuai, Yujun, Zhang, Hui, Liu, Changhao, Chen, Hebing, Jiang, Guosong. 2024. CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression. In Cell death & disease, 15, 9. doi:10.1038/s41419-023-06373-z. https://pubmed.ncbi.nlm.nih.gov/38182571/
2. Kawai, Shunsuke, Fujii, Takuto, Shimizu, Takahiro, Sakai, Hideki, Fujii, Tsutomu. 2020. Pathophysiological properties of CLIC3 chloride channel in human gastric cancer cells. In The journal of physiological sciences : JPS, 70, 15. doi:10.1186/s12576-020-00740-7. https://pubmed.ncbi.nlm.nih.gov/32066374/
3. Macpherson, Iain R, Rainero, Elena, Mitchell, Louise E, Timpson, Paul, Norman, Jim C. 2014. CLIC3 controls recycling of late endosomal MT1-MMP and dictates invasion and metastasis in breast cancer. In Journal of cell science, 127, 3893-901. doi:10.1242/jcs.135947. https://pubmed.ncbi.nlm.nih.gov/25015290/
4. Murthi, P, Stevenson, J L, Money, T T, Brennecke, S P, Gude, N M. 2012. Placental CLIC3 is increased in fetal growth restriction and pre-eclampsia affected human pregnancies. In Placenta, 33, 741-4. doi:10.1016/j.placenta.2012.06.011. https://pubmed.ncbi.nlm.nih.gov/22795578/
5. Zhu, Xiaoli, Wang, Chuan-Lan, Yu, Jian-Feng, Tang, Xiaowei, Pan, Bo. 2023. Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms. In Frontiers in cellular neuroscience, 17, 1256184. doi:10.3389/fncel.2023.1256184. https://pubmed.ncbi.nlm.nih.gov/37841288/
6. Luan, Changjiao, Gao, Yue, Zhao, Jun, Gong, Weijuan, Ma, Xingjie. 2025. Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. In Communications biology, 8, 51. doi:10.1038/s42003-025-07482-5. https://pubmed.ncbi.nlm.nih.gov/39809890/
7. Dozynkiewicz, Marta A, Jamieson, Nigel B, Macpherson, Iain, Caswell, Patrick T, Norman, Jim C. 2011. Rab25 and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression. In Developmental cell, 22, 131-45. doi:10.1016/j.devcel.2011.11.008. https://pubmed.ncbi.nlm.nih.gov/22197222/
8. Wang, Zhiming, Ling, Shizhang, Rettig, Eleni, Fakhry, Carole, Ha, Patrick K. 2015. Epigenetic screening of salivary gland mucoepidermoid carcinoma identifies hypomethylation of CLIC3 as a common alteration. In Oral oncology, 51, 1120-5. doi:10.1016/j.oraloncology.2015.09.010. https://pubmed.ncbi.nlm.nih.gov/26490796/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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