C57BL/6JCya-Clic3em1/Cya
Common Name:
Clic3-KO
Product ID:
S-KO-20855
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Clic3-KO
Strain ID
KOCMP-69454-Clic3-B6J-VB
Gene Name
Product ID
S-KO-20855
Gene Alias
2300003G24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clic3em1/Cya mice (Catalog S-KO-20855) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114265
NCBI RefSeq
NM_027085
Target Region
Exon 2~6
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
CLIC3, chloride intracellular channel 3, belongs to the glutathione-S-transferase (GSTs) superfamily. It regulates fundamental cellular processes like chloride ion concentration regulation, cell membrane potential stabilization, and is involved in trans-epithelial transport, cell volume regulation, and apoptotic processes [4].
In cancer research, CLIC3 has been found to be significantly involved. In bladder cancer, it promotes cell proliferation by reducing p21 expression through interacting with NAT10 and inhibiting N4-acetylcytidine modification of p21 mRNA, indicating its potential as a therapeutic target [1]. In breast cancer, CLIC3 controls the recycling of MT1-MMP from late endosomes to sites of cell-matrix adhesion, and its knockdown opposes MT1-MMP-dependent invasive processes, predicting poor prognosis in ER-negative breast cancer [3]. In pancreatic ductal adenocarcinoma, CLIC3, collaborating with Rab25, promotes integrin recycling from late endosomes/lysosomes, and its expression predicts lymph node metastasis and poor prognosis [7]. In gastric cancer, CLIC3 functions as a Cl-channel in the plasma membrane, and decreased expression is associated with unfavorable prognosis [2]. In salivary gland mucoepidermoid carcinoma, CLIC3 promoter hypomethylation and overexpression are significant events [8].
In schizophrenia research, CLIC3 was identified as an immune-related hub gene, potentially a diagnostic biomarker and therapeutic target, as its levels were reduced in a schizophrenia rat model and reversed by an antipsychotic drug [5]. Also, in fibroblast cellular senescence, knockdown of CLIC3 mitigated various senescence-related phenotypes by interacting with ERK7 [6].
In conclusion, CLIC3 is involved in multiple biological processes and disease conditions. Its roles in cancer progression, including bladder, breast, pancreatic, gastric, and salivary gland cancers, are well-studied. In addition, it has potential implications in schizophrenia and fibroblast cellular senescence. Research on CLIC3 using gene-knockout or other loss-of-function models has provided valuable insights into its functions in these disease areas, helping to understand the underlying molecular mechanisms and potentially guiding new therapeutic strategies.
References:
1. Shuai, Yujun, Zhang, Hui, Liu, Changhao, Chen, Hebing, Jiang, Guosong. 2024. CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression. In Cell death & disease, 15, 9. doi:10.1038/s41419-023-06373-z. https://pubmed.ncbi.nlm.nih.gov/38182571/
2. Kawai, Shunsuke, Fujii, Takuto, Shimizu, Takahiro, Sakai, Hideki, Fujii, Tsutomu. 2020. Pathophysiological properties of CLIC3 chloride channel in human gastric cancer cells. In The journal of physiological sciences : JPS, 70, 15. doi:10.1186/s12576-020-00740-7. https://pubmed.ncbi.nlm.nih.gov/32066374/
3. Macpherson, Iain R, Rainero, Elena, Mitchell, Louise E, Timpson, Paul, Norman, Jim C. 2014. CLIC3 controls recycling of late endosomal MT1-MMP and dictates invasion and metastasis in breast cancer. In Journal of cell science, 127, 3893-901. doi:10.1242/jcs.135947. https://pubmed.ncbi.nlm.nih.gov/25015290/
4. Murthi, P, Stevenson, J L, Money, T T, Brennecke, S P, Gude, N M. 2012. Placental CLIC3 is increased in fetal growth restriction and pre-eclampsia affected human pregnancies. In Placenta, 33, 741-4. doi:10.1016/j.placenta.2012.06.011. https://pubmed.ncbi.nlm.nih.gov/22795578/
5. Zhu, Xiaoli, Wang, Chuan-Lan, Yu, Jian-Feng, Tang, Xiaowei, Pan, Bo. 2023. Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms. In Frontiers in cellular neuroscience, 17, 1256184. doi:10.3389/fncel.2023.1256184. https://pubmed.ncbi.nlm.nih.gov/37841288/
6. Luan, Changjiao, Gao, Yue, Zhao, Jun, Gong, Weijuan, Ma, Xingjie. 2025. Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. In Communications biology, 8, 51. doi:10.1038/s42003-025-07482-5. https://pubmed.ncbi.nlm.nih.gov/39809890/
7. Dozynkiewicz, Marta A, Jamieson, Nigel B, Macpherson, Iain, Caswell, Patrick T, Norman, Jim C. 2011. Rab25 and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression. In Developmental cell, 22, 131-45. doi:10.1016/j.devcel.2011.11.008. https://pubmed.ncbi.nlm.nih.gov/22197222/
8. Wang, Zhiming, Ling, Shizhang, Rettig, Eleni, Fakhry, Carole, Ha, Patrick K. 2015. Epigenetic screening of salivary gland mucoepidermoid carcinoma identifies hypomethylation of CLIC3 as a common alteration. In Oral oncology, 51, 1120-5. doi:10.1016/j.oraloncology.2015.09.010. https://pubmed.ncbi.nlm.nih.gov/26490796/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen