Save Up To 15% On Custom Animal Models

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If you need immediate assistance, please email us at
animal-service@cyagen.com.

Cyagen understands that the uncertainties posed by the COVID-19 pandemic has been challenging for researchers worldwide. To support the continuity of your research progress, we would like to offer a personalized savings promotion for our custom animal model (CAM) generation services.

mouse model generation services | Cyagen

For the Mix-and-Maximize Savings Event, our Model Design Specialist (MDS) team will work with you to identify your project needs and generate a quote that provides you with the maximum savings across a range of model generation services. Contact us to see how you can save up to 15% on your CAM project and even receive an additional $2k credit for add-on services – over $6,700 in savings available for TurboKnockout^® projects.

Custom Animal Model Savings – Maximum Discounts Available*:

● Save up to 15% on your CAM projects: TurboKnockout^®, ESC gene targeting, CRISPR, transgenic, PiggyBac transgenic, etc. Check "Custom Animal Model Capabilities" to learn more details.

● Up to $2k credit towards add-on services: downstream (homozygous) breeding, cryopreservation, and more

*Maximum discounts vary depending on CAM project type(s). For example, transgenic services may only receive a maximum of 5% off.

● Promotion Period: Offer ends September 30, 2020; quote must be requested before the deadline.

● Eligibility: Academic customers in US & EU – use promotional code "MIXMAX" when placing inquiry. Contact us for eligibility in other regions or for commercial use.

Our MDS representatives are standing by to provide you with a complimentary animal model project consultation and custom quote – including discounts tailored to provide the greatest savings for your research plan. Request a quote before September 30, 2020 to qualify for the Mix-and-Maximize Savings Event.

Do you need temporary assistance getting your project started? Contact us to see how our Model Design Specialists can help you.

TurboKnockout^® - Proprietary Method for Accelerated Generation of Precise Mouse Models

Cyagen’s Proprietary Method for Accelerated Generation of Precise Mouse Models

Our proprietary TurboKnockout^® gene targeting method can provide you with conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months. This proprietary gene targeting method is enabled by two innovations:

● Eliminates the unpredictability of germline transmission (GLT)with our super competent ESC line that generates 100% ESC-derived founder mice rather than chimeras.

● Self-removing selection cassette circumvents the need to breed to Flp deleter mice

These innovations eliminate at least two generations of breeding, shortening production time by 4-6 months as compared to the industry standard

Highlights of TurboKnockout^® Gene Targeting

TurboKnockout^® gene targeting: 6-8 months

Targeting Vector
Construction
2 months
Gene Targeting
in ES Cells
2-3 months
Founder Het
Generation
2-3 months

Traditional gene targeting: 10-14 months

Targeting Vector
Construction
2 months
Gene Targeting
in ES Cells
2-3 months
Chimera
Generation
2-3 months
F1 Het
Generation
2-3 months
Removal of
Selection Cassette
2-3 months

Highlights of TurboKnockout^® Gene Targeting

● No patent risk: Technology of choice for drug development projects
● Success rate: 100% guaranteed germline transmission (GLT)
● Turnaround time: Founders as fast as 6-8 months
● Large fragment knockin size: Knockin (KI) fragment size up to 200 kb
● Limitless loxP: No size limitations for your floxed region
● 100% Money-Back Guarantee on TurboKnockout^® projects

Capabilities

Knockout/Conditional Knockout/Conditional Knockin/Knockin/Humanized | Cyagen

Donor Strains Mouse: C57BL/6, BALB/c

TurboKnockout^® for BALB/c Drug Development Models

BALB/c is a laboratory-bred, albino strain of the house mouse, characterized by its immunodeficiency and extreme susceptibility to carcinogens. BALB/c mice are among the most widely used mouse strains across oncology, immunology, nuclear medicine research, and are often the first option for animal experimentation models in new drug development projects.

TurboKnockout^® is based on traditional embryonic stem cell (ESC) targeting techniques, which can be used for complex genetic modification projects to provide BALB/c mouse models and is the technology of choice among our clients performing drug development projects.

Cyagen CRISPR-Pro Technology - Capabilities for Mouse & Rat Models

Engineered nuclease-mediated genome editing (especially CRISPR/Cas) is an emerging technology which can serve as an alternative to the conventional, ES cell homologous recombination-based knockout (KO) animal generation. These methods have enabled the generation of custom mouse and rat models with genetic knockouts, point mutations, and large fragment knockins (LFKIs).

Cyagen’s CRISPR-based Rapid Knockout services use accelerated methodology that provides conventional knockout, reporter knockin, and humanization founder animals in as fast as 3 months.

Capabilities

KO/cKO/Point Mutation/Large Fragment Knockin/Rosa26 Targeted Transgenic | Cyagen

Donor Strain Information:

● Mouse strains: C57BL/6, FVB
● Rat strains: Sprague-Dawley (SD), Long Evans
Note: Other strains available upon request.

Transgenic Capabilities for Animal Models

Items	Regular Transgenic	PiggyBac Transgenic	Rosa26 Targeted Transgenic
Integration	Random, multicopy integration	Random, single copy per integration site	Single copy transgene targeted to Rosa26 safe harbor locus
Vector construction	Plasmid or BAC	Plasmid or BAC	CRISPR or TurboKnockout^®
Expression pattern	Variable	More consistent	Most consistent
Endogenous effects	Can disrupt endogenous genes	Less likely to disrupt endogenous genes	Safe harbor locus
Donor Strain Information	Mouse strains: C57BL/6, FVB Rat strains: Sprague-Dawley (SD), Long Evans Note: Other strains available upon request.

PiggyBac transgenic services can deliver single-copy transgenic animals with more consistent expression of your transgene. This proprietary transgenic method has the following advantages over other transgenic approaches:

◆ Single-copy integration: Avoids potential gene silencing from multiple copies
◆ Defined region of integration: No loss of transgene sequence (TTAA, transcription unit)
◆ Reliability: More consistent expression pattern compared to plasmid based transgenics
◆ Economical: Cost and turnaround time comparable to plasmid based transgenics

Cyagen Model Generation Expertise

14+ years’ experience in custom animal model generation

50,000+ animal models generated and delivered worldwide

4,000+ citations in SCI journals for our services

3,000+ university and company partners

Customer Testimonials

"We generated a mouse knockout model using Cyagen’s TurboKnockout^® technology. Cyagen’s team was on time with milestones, provided constant updates on the project and delivered animals as promised. It was such a pleasure working with Cyagen that we decided to outsource the production of other animal models to them."

-- Maximiliano D’Angelo, Sanford Burnham Prebys Medical Discovery Institute

Mix-and-Maximize Savings Event for Custom Models

Custom Animal Model Savings – Maximum Discounts Available*:

TurboKnockout® - Proprietary Method for Accelerated Generation of Precise Mouse Models

Highlights of TurboKnockout® Gene Targeting

Highlights of TurboKnockout® Gene Targeting

Capabilities

Donor Strains Mouse: C57BL/6, BALB/c

TurboKnockout® for BALB/c Drug Development Models

Cyagen CRISPR-Pro Technology - Capabilities for Mouse & Rat Models

Capabilities

Donor Strain Information:

Transgenic Capabilities for Animal Models

Cyagen Model Generation Expertise

TurboKnockout^® - Proprietary Method for Accelerated Generation of Precise Mouse Models

Highlights of TurboKnockout^® Gene Targeting

Highlights of TurboKnockout^® Gene Targeting

TurboKnockout^® for BALB/c Drug Development Models