1. Home
  2. Community
  3. Promotions
  4. Cyagen's Year-End Savings: Save up to 10% on custom animal models

Save up to $6,310 on custom mouse and rat model | Cyagen

If you are seeking to generate a custom rodent model for your research project, it is the perfect time to lock-in savings and ensure funds don’t go to waste.

Cyagen's Year-End Savings Event can help you extend your research with big savings for multiple projects - save up to 10% off custom animal models for research. The more mouse models you order, the more you save!

Pay Now – Start Later

We can initiate projects to meet your project timeline – so you can lock-in promotional prices on multiple models and decide project details later. Our Model Design Specialists can provide complimentary project strategy details within 24 hours and initiate your project as soon or as late as you require.

Refer a colleague

Waiting on funding? Refer a colleague now to receive a coupon for these limited-time discounts that you may apply when your grant arrives. Referral coupon will be provided after the referred project is placed during the promotional period - before December 18, 2020. To refer a colleague, simply send us an email at animal-service@cyagen.com with a brief statement indicating whom is being referred (please copy all referred colleagues).

Cyagen's Year-End Savings Event Details

Save up to $6310 on custom animal models | Cyagen

Promotion Period: Offer ends December 18, 2020.

Eligibility: Academic end clients in Europe, North America, and South America. Promotional pricing is only available for new projects with new clients and may not be applied to past inquiries or projects in-progress.

The offer applies to the following eligible custom mouse and rat model services:

Custom Mouse and Rat Model Services
Model Generation Services Species 1 Project ≥2 Projects Deliverables
ES Cell Mediated TurboKnockout Mouse only 5% off 10% off ≥3 F1 animals
CRISPR/Cas9 - based Knockout (KO) Mouse or Rat 5% off 10% off ≥3 F1 animals
KI (any locus)
KI (ROSA26)
Point mutation
cKO
Transgenic Regular Transgenic Mouse or Rat 5% off ≥3 founders
(plasmid injection)
or ≥2 founders
(BAC injection)
PiggyBac Transgenic
PiggyBac-on-BAC Transgenic

Note

  • ①  Any of the listed services may count towards the total discounts received, up to 10% off per project. Transgenic services are only eligible for 5% off the list price but may count towards the total number of projects - increasing discounts for all other eligible services listed.
  • ②  Listed prices do not include handling fees or Taconic's RapidCheck Health Certification Program.

About Cyagen

Cyagen's experts have generated thousands of models and offer 100% money back guarantee on standard projects - no animals, no charge.
Cyagen's experts have generated thousands of models and offer 100% money back guarantee on standard projects - no animals, no charge.

TurboKnockout® - Proprietary Method for Accelerated Generation of Precise Mouse Models

Cyagen's Proprietary Method for Accelerated Generation of Precise Mouse Models

Our proprietary TurboKnockout® gene targeting method can provide you with conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months. This proprietary gene targeting method is enabled by two innovations:

Eliminates the unpredictability of germline transmission (GLT)with our super competent ESC line that generates 100% ESC-derived founder mice rather than chimeras.

Self-removing selection cassette circumvents the need to breed to Flp deleter mice.

These innovations eliminate at least two generations of breeding, shortening production time by 4-6 months as compared to the industry standard.

Faster Turnaround with Turboknockout®

TurboKnockout® gene targeting: 6-8 months

  • Targeting Vector
    Construction

    2 months
  • Gene Targeting
    in ES Cells

    2-3 months
  • Founder Het
    Generation

    2-3 months

Traditional gene targeting: 10-14 months

  • Targeting Vector
    Construction

    2 months
  • Gene Targeting
    in ES Cells

    2-3 months
  • Chimera
    Generation

    2-3 months
  • F1 Het
    Generation

    2-3 months
  • Removal of
    Selection Cassette

    2-3 months

Highlights of TurboKnockout® Gene Targeting

No patent risk: Technology of choice for drug development projects
Success rate: 100% guaranteed germline transmission (GLT)
Turnaround time: Founders as fast as 6-8 months
Large fragment knockin size: Knockin (KI) fragment size up to 200 kb
Limitless loxP: No size limitations for your floxed region
100% Money-Back Guarantee on TurboKnockout® projects

Capabilities

Knockout, Conditional Knockout, Knockin, Conditional Knockin and Humanized | Cyagen

Donor Strains Mouse: C57BL/6, BALB/c

TurboKnockout® for BALB/c Drug Development Models

BALB/c is a laboratory-bred, albino strain of the house mouse, characterized by its immunodeficiency and extreme susceptibility to carcinogens. BALB/c mice are among the most widely used mouse strains across oncology, immunology, nuclear medicine research, and are often the first option for animal experimentation models in new drug development projects.

TurboKnockout® is based on traditional embryonic stem cell (ESC) targeting techniques, which can be used for complex genetic modification projects to provide BALB/c mouse models and is the technology of choice among our clients performing drug development projects.

Cyagen CRISPR-Pro Technology - Capabilities for Mouse & Rat Models

Engineered nuclease-mediated genome editing (especially CRISPR/Cas9) is an emerging technology which can serve as an alternative to the conventional, ES cell homologous recombination-based knockout (KO) animal generation. These methods have enabled the generation of custom mouse and rat models with genetic knockouts, point mutations, and large fragment knockins (LFKIs).

Cyagen's CRISPR-based Rapid Knockout services use accelerated methodology that provides conventional knockout, reporter knockin, and humanization founder animals in as fast as 3 months.

Capabilities

KO, cKO, Point Mutation/tag, Large Fragment Knockin, Rosa26 Targeted Transgenic | Cyagen

Donor Strain Information:

Mouse strains: C57BL/6, FVB
Rat strains: Sprague-Dawley (SD), Long Evans
Note: Other strains available upon request.

Transgenic Capabilities for Animal Models
Items Regular Transgenic PiggyBac Transgenic Rosa26 Targeted Transgenic
Integration Random, multicopy integration Random, single copy per integration site Single copy transgene targeted to Rosa26 safe harbor locus
Vector construction Plasmid or BAC Plasmid or BAC CRISPR or TurboKnockout®
Expression pattern Variable More consistent Most consistent
Endogenous effects Can disrupt endogenous genes Less likely to disrupt endogenous genes Safe harbor locus
Donor Strain Information Mouse strains: C57BL/6, FVB
Rat strains: Sprague-Dawley (SD), Long Evans
Note: Other strains available upon request.

PiggyBac transgenic services can deliver single-copy transgenic animals with more consistent expression of your transgene. This proprietary transgenic method has the following advantages over other transgenic approaches:

Single-copy integration: Avoids potential gene silencing from multiple copies
Defined region of integration: No loss of transgene sequence (TTAA, transcription unit)
Reliability: More consistent expression pattern compared to plasmid based transgenics
Economical: Cost and turnaround time comparable to plasmid based transgenics

Cyagen Model Generation Expertise
14+ years' experience in custom animal model generation
50,000+ animal models generated and delivered worldwide
4,000+ citations in SCI journals for our services
3,000+ university and company partners
Customer Testimonials

"We generated a mouse knockout model using Cyagen’s TurboKnockout® technology. Cyagen's team was on time with milestones, provided constant updates on the project and delivered animals as promised. It was such a pleasure working with Cyagen that we decided to outsource the production of other animal models to them."

-- Maximiliano D’Angelo, Sanford Burnham Prebys Medical Discovery Institute