C-NKG mice

C-NKG mice

 

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Strain Name: NOD/Shi-Prkdcscid Il2rgem1/Cyagen

Abbreviation: C-NKG Mouse

Strain Background: NOD/Shi-Scid

C-NKG mice are a severe combined immunodeficiency (SCID) model developed by Cyagen via CRISPR/Cas9-mediated IL2RG gene knockout (KO) on the NOD/Shi-Scid background strain. C-NKG mice lack mature T, B, and natural killer (NK) immune cells, so the complement activity is reduced and macrophage phagocytosis of human derived cells is weakened. This model is compatible with transplanted hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), patient-derived xenografts (PDXs) or adult stem cells and tissues. The C-NKG mouse model is recognized as having high degree of immunodeficiency and has good performance in the study of tumors, immunity, autoimmunity diseases, immunotherapy vaccine, GVHD/transplantation, safety evaluations, and more.

C-NKG Features:

● Lacks mature T, B, and NK cells

● Decreased complement activity

● Dysfunction of macrophages and dendritic cells

● Extremely low incidence of T and B cell leakage with age

● Extremely low incidence of lymphoma (different from NOD SCID model)

● Can be used in both long-term and short-term experiments

● Does not develop diabetes

Research & Application:

● Compared with NOD SCID mice, C-NKG mice have significantly higher survival rate after transplantation of human cells and tissues and allow implantation of a higher proportion of normal or cancerous human cells and tissues.

● High efficiency in transplantation of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), patient-derived xenografts (PDXs) or adult stem cells and tissues.

● May be used for research on tumors, immunity, autoimmune diseases, immunotherapy vaccines, GvHD/transplantation, safety evaluations, and more.

Phenotype Validation:

1. Prkdc gene mutation

Figure 1. Prkdcscid mutation is produced by TAT → TAA in exon 84 of Prkdc gene. The sequencing result shows that C-NKG mice carry Prkdcscid mutation.

 

2. Il2rg gene knockout

Figure 2. The II2rg gene of NKG mouse was detected by PCR and the result shows that the II2rg gene of NKG mouse was successfully knocked out. The band size of wild type was 1430 bp and that of the knockout fragment was 380 bp.

 

3. Detection of B, T, and NK cells in peripheral blood of C-NKG mouse

Figure 3. The peripheral blood of C-NKG mouse, a severe combined immunodeficiency (SCID) mouse model, is severely deficient in B, T, and NK cells. Peripheral blood samples of BALB/c and C-NKG mice were collected, followed by the performance of flow cytometric immunophenotypic analysis and statistical comparison of the composition of T, B and NK cells. The results show that B cells (CD3-CD19+), T cells (CD3+CD19-), helper T cells (CD3+CD4+CD8-), cytotoxic T cells (CD3+CD4-CD8+) and NK cells (CD335+CD3-) in the peripheral blood of C-NKG mice were almost completely absent while comparing with BALB/c mice.

 

4. Detection of B, T, and NK cells in lymphoid tissue of C-NKG mice

Figure 4. The lymphoid tissue of C-NKG mouse, a severe combined immunodeficiency (SCID) mouse model, is severely deficient in B, T and NK cells. Lymphoid tissue samples of BALB/cand C-NKGmice were collected, followed by flow cytometric immunophenotypic analysis and statistical comparison of the composition of T, B and NK cells. The results show that B cells (CD3-CD19+), T cells (CD3+CD19-), helper T cells (CD3+CD4+CD8-), cytotoxic T cells (CD3+CD4-CD8+) and NK cells (CD335+CD3-) in the lymphoid tissue of C-NKGmice were almost completely absent, ascompared with BALB/cmice.