Item Number: C001316
Strain Name: NOD-Prkdcscid Il2rgem1/Cyagen
C-NKG mice are a severe immunodeficiency model independently developed by Cyagen on the NOD-Scid background strain to knock out the Il2rg gene. C-NKG mice lack mature T, B, and natural killer (NK) immune cells, so the complement activity is reduced and phagocytosis of macrophage on human cells is weakened. This model is compatible with transplanted hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), patient-derived xenografts (PDXs), or adult stem cells and tissues. The C-NKG mouse model is recognized as having high degree of immunodeficiency and has good performance in the study of tumors, immunity, autoimmune diseases, immunotherapy vaccine, graft-versus-host disease (GVHD) , safety evaluations, and more.
● Lacks mature T, B, and NK cells
● Decreased complement activity
● Dysfunction of macrophages and dendritic cells
● Extremely low incidence of T and B cell leakage with age
● Extremely low incidence of lymphoma (different from NOD SCID model)
● Can be used in both long-term and short-term experiments
● Does not develop diabetes
● Compared with NOD SCID mice, C-NKG mice have significantly higher survival rate after transplantation of human cells and tissues and allow implantation of a higher proportion of normal or cancerous human cells and tissues.
● High efficiency in transplantation of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), patient-derived xenografts (PDXs) or adult stem cells and tissues.
● May be used for research on tumors, immunity, autoimmune diseases, immunotherapy vaccines, GvHD/transplantation, safety evaluations, and more.
1. Prkdc gene mutation
Figure 1. Prkdcscid mutation is produced by TAT → TAA in exon 84 of Prkdc gene.
The sequencing result shows that C-NKG mice carry Prkdcscid mutation.
2. Il2rg gene knockout
Figure 2. The II2rg gene of NKG mouse was detected by PCR and the result shows that the II2rg gene of NKG mouse was successfully knocked out.
The band size of wild type was 1430 bp and that of the knockout fragment was 388 bp.
3. Detection of B, T, and NK cells in peripheral blood of C-NKG mouse
Figure 3. The peripheral blood of C-NKG mouse, a severe combined immunodeficiency (SCID) mouse model, is severely deficient in B, T, and NK cells.
Peripheral blood samples of BALB/c and C-NKG mice were collected, followed by the performance of flow cytometric immunophenotypic analysis and statistical comparison of the composition of T, B and NK cells. The results show that B cells (CD3-CD19+), T cells (CD3+CD19-), helper T cells (CD3+CD4+CD8-), cytotoxic T cells (CD3+CD4-CD8+) and NK cells (CD335+CD3-) in the peripheral blood of C-NKG mice were almost completely absent while comparing with BALB/c mice.
4. Detection of B, T, and NK cells in lymphoid tissue of C-NKG mice
Figure 4. The lymphoid tissue of C-NKG mouse, a severe combined immunodeficiency (SCID) mouse model, is severely deficient in B, T and NK cells.
Lymphoid tissue samples of BALB/cand C-NKGmice were collected, followed by flow cytometric immunophenotypic analysis and statistical comparison of the composition of T, B and NK cells. The results show that B cells (CD3-CD19+), T cells (CD3+CD19-), helper T cells (CD3+CD4+CD8-), cytotoxic T cells (CD3+CD4-CD8+) and NK cells (CD335+CD3-) in the lymphoid tissue of C-NKGmice were almost completely absent, ascompared with BALB/cmice.
Figure 1. Survival curve of huPBMC-C-NKG mice.
The huPBMC-C-NKG mice gradually died with the onset of graft-versus-host disease (GvHD).
Figure 2. Changes in the content of human CD45+ cells in peripheral blood after the transplantation of human PBMCs into C-NKG mice.
After PBMC transplantation, the content of human leukocytes in the peripheral blood of C-NKG mice gradually increased, and the average proportion of CD45+ was about 50% after 3 weeks.
Figure 3. Changes in the content of human CD3+ cells in peripheral blood after transplantation of human PBMCs into C-NKG mice.
The reconstitution of huPBMC-C-NKG mice was dominated by T cells.
6. Human tumor cell line xenograft (CDX) model
Figure 1. Tumor growth curve of human hepatoma cell Huh7 subcutaneous xenograft
Figure 2. Tumor growth curve of human gastric cancer cell HGC-27 subcutaneous xenograft.
Figure 3. Human colon adenocarcinoma SW620 subcutaneous xenograft tumor growth curve
Cells were subcutaneously inoculated into both C-NKG and NOD/Scid mice, and tumor volume was measured at different time points. The cell seeding amount was 5×106/cell and the data were presented in the form of Mean±SEM. The results showed that human liver cancer cells Huh7, human gastric cancer cells HGC-27 and human colon adenocarcinoma cells SW620 could effectively establish tumor models in C-NKG mice.
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