Our new PiggyBac-on-BAC transgenic mouse service can deliver single-copy transgenic mice with more consistent expression of your transgene. This proprietary transgenic method has the following advantages over other transgenic approaches allowing for increased reliability and consistency in expression:
(1) Integration occurs as a single copy at each integration site. By contrast, in conventional transgenics, there are typically multiple copies integrated in tandem at each integration site. The presence of multiple copies can interfere with the expression of the transgene, and may even induce silencing.
(2) The integrated fragment is defined, namely only your desired transgene sequence is integrated. No vector backbone sequence is integrated, nor is there any loss of your transgene sequence. By contrast, in conventional transgenics, the transgene may integrate along with extra vector backbone sequence, or the transgene sequence may be partially eroded.
Each full PiggyBac-on-BAC transgenic mouse project is divided into the following phases:
The success of PiggyBac-on-BAC transgenic production depends critically on the design and the quality of PiggyBac-BAC used in pronuclear injection. Accordingly, we employ a special protocol to design and prepare top-quality PiggyBac-BAC for our injections. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC.
-PiggyBac-BAC injection to obtain founders, basic service: A minimum of 200 eggs (C57BL/6) will be injected to produce live animals. This will result in an average of 2 transgenic founders, but the actual numbers could fluctuate significantly, and it is possible that no founders are obtained. If you wish to ensure the generation of founders, we strongly recommend that you use our guaranteed service option (see below).
-PiggyBac-BAC injection to obtain founders, guaranteed service: We guarantee a minimum of 2 transgenic founders (the actual number of founders obtained is typically higher).
-Genotyping pups to identify founders: Pups will be genotyped by PCR to identify PiggyBac-on-BAC transgenic founder mice following the strategy designed by Cyagen scientists.
|Service||Donor egg strain||Price||Turnaround time|
PiggyBac-on-BAC transgenic strategy design
|PiggyBac-BAC modification||Please inquire||2-5 weeks|
|Prepare PiggyBac BAC for injection and develop genotyping strategy||$600||1-2 weeks|
|PiggyBac-BAC injection to obtain founders, basic service||C57BL/6||$5,950||10-14 weeks|
|PiggyBac-BAC injection to obtain founders, guaranteed service||C57BL/6||$8,950||10-18 weeks|
|Genotyping pups to identify PiggyBac-on-BAC transgenic founders||$450||1 week|
We typically produce PiggyBac-on-BAC transgenic mice in C57BL/6 strain backgrounds, but we may be able to offer other strains to accommodate your special research needs.
>> Rat models are also available. Learn more about PiggyBac-on-BAC Transgenic Rat Services.
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.
Please see below for supporting information related to Cyagen animal orders.