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Our new PiggyBac transgenic mouse service can deliver single-copy transgenic mice with more consistent expression of your transgene. This proprietary transgenic method has the following advantages over other transgenic approaches:
(1) Integration occurs as a single copy at each integration site. By contrast, in conventional transgenics, there are typically multiple copies integrated in tandem at each integration site. The presence of multiple copies can interfere with the expression of the transgene, and may even induce silencing.
(2) The integrated fragment is defined, namely only your desired transgene sequence is integrated. No vector backbone sequence is integrated, nor is there any loss of your transgene sequence. By contrast, in conventional transgenics, the transgene may integrate along with extra vector backbone sequence, or the transgene sequence may be partially eroded.
Workflow of PiggyBac Transgenic Mouse Projects
Each full PiggyBac transgenic mouse project is divided into the following phases:
1. PiggyBac transgenic strategy design
- Design a PiggyBac transgenic strategy that meets your research needs.
- Develop a PCR genotyping strategy for PiggyBac transgenic founder screening.
2. PiggyBac transgenic vector construction
- Construct PiggyBac plasmid-based transgenic vectors.
- Confirm the constructed PiggyBac plasmid-based transgenic vectors by restriction digest and sequencing, as needed.
3. PiggyBac transgenic pronuclear injection
- Inject the verified PiggyBac transgenic plasmid into the pronucleus of fertilized eggs.
- Implant injected eggs into surrogate mothers to obtain offspring.
4. PiggyBac transgenic mouse screening
- Genotype pups by PCR to identify those carrying the PiggyBac transgene.
Technical Details of PiggyBac Transgenic Mice
Preparation of DNA for injection and development of a genotyping strategy
The success of PiggyBac transgenic production depends critically on the design and the quality of PiggyBac vector used in pronuclear injection. Accordingly, we employ a special protocol to design and prepare top-quality PiggyBac vectors for our injections. A PCR-based genotyping strategy will be developed and tested.
Generation of PiggyBac transgenic founder mice
- PiggyBac plasmid injection to obtain founders, basic service: A minimum of 400 eggs (C57BL/6) will be injected to produce live animals. This will result in an average of 3-4 transgenic founders, but the actual numbers could fluctuate significantly, and it is possible that no founders are obtained. If you wish to ensure the generation of founders, we strongly recommend that you use our guaranteed service option (see below).
- PiggyBac plasmid injection to obtain founders, guaranteed service: We guarantee a minimum of 4 PiggyBac transgenic founders (the actual number of founders obtained is typically higher).
-Genotyping pups to identify founders: Pups will be genotyped by PCR to identify PiggyBac transgenic founder mice following the strategy designed by Cyagen scientists.
Pricing and Turnaround Time
| Service | Donor egg strain | Price | Turnaround time |
|---|---|---|---|
|
PiggyBac transgenic strategy design |
Free | 1-4 days | |
| Transgenic vector construction (PiggyBac plasmid) |
Please inquire | 2-5 weeks | |
| Prepare PiggyBac DNA for injection and develop genotyping strategy | $600 | 1-2 weeks | |
| PiggyBac plasmid injection to obtain founders, basic service | C57BL/6 | $4,950 | 8-10 weeks |
| PiggyBac plasmid injection to obtain founders, guaranteed service | C57BL/6 | $6,950 | 8-16 weeks |
| Genotyping pups to identify PiggyBac transgenic founders | $350 | 1 week |
Note: For PiggyBac transgenic mouse services not listed above, please inquire about availability and pricing. The turnaround time above does not include the time for obtaining host institution’s approval for mouse importation, nor transit time during shipping.
Donor Strain Information
We typically produce PiggyBac transgenic mice in C57BL/6 strain backgrounds, but we may be able to offer other strains to accommodate your special research needs.
>> Rat models are also available. Learn more about PiggyBac Transgenic Rat Services.
Guarantee
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.
Order Supporting Information
Please see below for supporting information related to Cyagen animal orders.
（https://www.cyagen.com/us/en/support/order-information.html）
Inquiries and Quote Requests
Request a quote now. Alternatively, you can always email animal-service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.
Why work with Cyagen?
- 14+ years' experience in custom animal model generation
- 50,000+ animal models generated and delivered worldwide
- 4,000+ citations in SCI journals for our services
- 3,000+ university and company partners
- 100% money-back guarantee option available for most services
- AAALAC-Accredited and OLAW Assured
