Item Number: C001197
Genotype: Homozygous Rag1 Knockout (KO)
The protein encoded by the Rag1 gene is involved in the activation of immunoglobulin V(D)J recombination. Rag1 is the catalytic component of the RAG complex, in which RAG1 mediates the binding of DNA to conserved recombination signal sequences (RSS) and catalyzes DNA cleavage activity by introducing double-strand breaks between the RSS and adjacent coding segments. Rag1 deletion was used in mouse models of disease to impair T and B cell development, and mouse models lacking the Rag1 gene had no mature T and B cells.
• Detection of B, T cells
Figure 1. Detection of T and B cells in peripheral blood, spleen and thymus of Rag1 KO mice.
The peripheral blood, spleen and thymus of Rag1 knockout (KO) and C57BL/6 mice were collected for representative flow cytometric immunophenotyping and statistical comparison of the composition of T and B cells. The results showed that B cells (CD3-CD19+) and T cells (CD3+CD19-) in the peripheral blood, spleen and thymus of Rag1 KO homozygous mice were almost completely absent compared with C57BL/6 mice.