Rag1 KO

Catalog Number: C001197

Strain Name: C57BL/6NCya-Rag1em1/Cya

Genetic Background: C57BL/6NCya

Reproduction: Homozygote x Homozygote

 

Strain Description

The V(D)J recombination activation gene RAG1 was isolated based on its ability to activate V(D)J recombination on an artificial substrate in fibroblasts. This property and the expression pattern in tissues and cell lines indicate that RAG1 either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes [1]. Diseases associated with RAG1 include Alpha/Beta T-cell Lymphopenia With Gamma/Delta T-cell Expansion, Severe Cytomegalovirus Infection, Autoimmunity, and Combined Cellular And Humoral Immune Defects With Granulomas [2].

The Rag1-KO Mouse is a RAG1-deficient model that exhibits smaller lymphoid organs and does not contain mature B and T lymphocytes, the arrest of B and T cell differentiation occurs at an early stage and correlates with the inability to perform V(D)J recombination. The homozygote Rag1-KO mice developed normally and were fertile.

 

Allele Type

The Rag1 gene is located on chromosome 2 of the mouse and contains 2 exons, the start codon ATG and the stop codon TAA are both located in Exon 2, and Exon 2 of the Rag1 gene is knocked out by gene editing technology.


Validation Data

1. Detection of T cells and B cell

Figure 1. Detection of T and B cells in peripheral blood (PB), spleen, and thymus of Rag1 KO mice. The peripheral blood (PB), spleen, and thymus of Rag1 KO and wild-type mice were collected, and the composition of T and B cells was analyzed by representative flow cytometric immunophenotype and statistical comparison. An almost complete absence of CD3-CD19+ B cells and CD3+CD19- T cells in peripheral blood (PB), spleen, and thymus of Rag1-KO homozygous mice compared to wild-type mice.

 

2. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in peripheral blood

Figure 2. Flow cytometry results of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the peripheral blood of Rag1 KO mice and wild-type (WT) mice (8-week-old, female, n=3). The results show that, compared with WT mice, the proportion of NK cells in Rag1 KO mice increased significantly. There was no significant difference in the proportions of monocytes, macrophages, and dendritic cells (DCs), while the proportion of neutrophils increased.

 

3. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the spleen

Figure 3. Flow cytometry results of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the spleens of Rag1 KO mice and wild-type (WT) mice (8-week-old, female, n=3). The results show that, compared with WT mice, the proportion of NK cells in Rag1 KO mice increased significantly. There was no significant difference in the proportion of monocytes, while the proportions of macrophages, neutrophils, and dendritic cells (DCs) increased.

 

4. Detection of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in bone marrow

Figure 4. Flow cytometry results of NK cells, monocytes, macrophages, neutrophils, and dendritic cells (DCs) in the bone marrow of Rag1 KO mice and wild-type (WT) mice (8-week-old, female, n=3). The results show that, compared with WT mice, the proportion of NK cells in Rag1 KO mice increased. There was no significant difference in the proportions of monocytes and macrophages, while the proportions of neutrophils and dendritic cells (DCs) increased slightly.

 

Publications

[1] Li F, Zhang Y, Li R, Li Y, Ding S, Zhou J, Huang T, Chen C, Lu B, Yu W, Boltze J, Li P, Wan J. Neuronal Serpina3n is an endogenous protector against blood brain barrier damage following cerebral ischemic stroke. J Cereb Blood Flow Metab. 2023 Feb;43(2):241-257.

References

[1]Mombaerts P, Iacomini J, Johnson RS, Herrup K, Tonegawa S, Papaioannou VE. RAG-1-deficient mice have no mature B and T lymphocytes. Cell. 1992 Mar 6;68(5):869-77.
[2]Bosticardo M, Pala F, Notarangelo LD. RAG deficiencies: Recent advances in disease pathogenesis and novel therapeutic approaches. Eur J Immunol. 2021 May;51(5):1028-1038.