Gene Aliases: TRA, TRA@
Mouse Status: Homozygous knockout mice are developmentally normal and fertile
T-cell receptors (TCRs) recognize foreign antigens that have been processed into small peptides and bound by the major histocompatibility complex (MHC) on the surface of antigen-presenting cells (APCs). Each T cell receptor is a dimer consisting of either an alpha chain and a beta chain, or a delta chain and a gamma chain. The TRA gene encodes the TCR alpha locus.
Homozygous mice with Tra knockout (KO) will lack the αβ T cell receptor and the thymus lacks CD4+CD8- and CD4-CD8+ cells, which retains about 1.5% of thymocytes that express the T cell receptor γδ.
Knockout of Trac gene (Gene ID: 100101484) exon 1-3.
Immunology; Inflammation; Autoimmunity Research.
• Detection of T cells in the thymus of Tra KO mice
Figure 1. Detection of T cells in the thymus of Tra KO mice
• Detection of T cells in blood and spleen of Tra KO mice
Figure 2. Detection of T cells in blood and spleen of Tra KO mice
The results showed that Tra KO mice lacked αβ T cell receptors after knockout of Tra gene, and the thymus did not develop CD4+CD8- and CD4-CD8+ single positive cells. Given that T cells include αβ T cells and γδ T cells, of which γδ T cells account for 0.5-5% of all T lymphocytes, the residual CD3+ T cells may be γδ T cells.