Tcra KO

 

>> Request a Quote or Information
Our Experts will contact you providing a quote,
information and estimated timeframe to
your project needs.

Gene Aliases: TRA, TRA@
Item Number:C001332
Background:C57BL/6J
Genotype:  Homozygous
Mouse Status: Homozygous knockout mice are developmentally normal and fertile

T-cell receptors (TCRs) recognize foreign antigens that have been processed into small peptides and bound by the major histocompatibility complex (MHC) on the surface of antigen-presenting cells (APCs). Each T cell receptor is a dimer consisting of either an alpha chain and a beta chain, or a delta chain and a gamma chain. The TRA gene encodes the TCR alpha locus.

Homozygous mice with Tra knockout (KO) will lack the αβ T cell receptor and the thymus lacks CD4+CD8- and CD4-CD8+ cells, which retains about 1.5% of thymocytes that express the T cell receptor γδ.

Knockout of Trac gene (Gene ID: 100101484) exon 1-3.

Immunology; Inflammation; Autoimmunity Research.

• Detection of T cells in the thymus of Tra KO mice


Figure 1. Detection of T cells in the thymus of Tra KO mice

• Detection of T cells in blood and spleen of Tra KO mice



Figure 2. Detection of T cells in blood and spleen of Tra KO mice

The results showed that Tra KO mice lacked αβ T cell receptors after knockout of Tra gene, and the thymus did not develop CD4+CD8- and CD4-CD8+ single positive cells. Given that T cells include αβ T cells and γδ T cells, of which γδ T cells account for 0.5-5% of all T lymphocytes, the residual CD3+ T cells may be γδ T cells.

 

Cyagen can provide you with a variety of drug evaluation models along with phenotype analysis services – delivering reliable and expedient data reporting for your project.

 

Inquiries and Quote Requests

Request a quote now. Alternatively, you can always email animal-service@cyagen.com or call 800-921-8930 to inquire about our services or obtain a quote for your project.

 

About Cyagen

● 15+ years' experience in custom animal model generation
● 78,000+ animal models generated and delivered worldwide
● 6,200+ citations in SCI journals
● 3,000+ university and company partners
 100% money-back guarantee option available for most services
● AAALAC-Accredited and OLAW Assured