Transgenic mice have foreign DNA incorporated into the mouse genome and are used to study a wide range of gene functions and human diseases. We offer a one-stop solution for all your transgenic mouse model needs. You can contract us to perform your entire transgenic project from initial strategy design and DNA vector construction to transgenic animal delivery, or you can outsource specific phases of the project to us. Our turnaround time is the shortest in the industry, our prices are unbeatable, and for guaranteed services, we guarantee the generation of animals or your money back.
Each full transgenic project is split into the following phases:
The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ a special protocol to prepare top-quality DNA for our injections. Plasmid DNA is typically linearized for injection. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC. A PCR-based genotyping strategy will be developed and tested. If you provide your own transgenic vector rather than having us construct it, you can send us either E. coli or unlinearized DNA in solution (≥50 µg total), and we will perform the necessary preparation to purify/linearize the DNA. We strongly discourage customers from sending us linearized, injection-ready DNA, because the quality of such DNA is often insufficient for pronuclear injection. If you provide your own vector without sequence information, you will also need to provide a PCR-based genotyping strategy along with your vector.
- Plasmid or BAC injection to obtain founders, basic service: A minimum of 400 eggs (C57BL/6) will be injected to produce live animals. This will result in an average of 2-3 transgenic founders in the case of plasmid injection or 1-2 founders in the case of BAC injection, but the actual numbers could fluctuate significantly and it is possible that no founders are obtained. If you wish to ensure the generation of founders, we strongly recommend that you use our guaranteed service option (see below).
- Plasmid or BAC injection to obtain founders, guaranteed service: We guarantee a minimum of 3 transgenic founders for plasmid injection or 2 founders for BAC injection (the actual number of founders obtained is typically higher).
- Genotyping pups to identify founders: Pups will be genotyped by PCR to identify founder mice.
|Service||Donor egg strain||Price||Turnaround time|
|Transgenic strategy design||Free||1-4 days|
|Transgenic vector construction or BAC modification*||Please inquire||2-5 weeks|
|Prepare DNA for injection and develop genotyping strategy||$500||1-2 weeks|
|Plasmid injection to obtain founders, basic service||C57BL/6||$3,450||6-10 weeks|
|Plasmid injection to obtain founders, guaranteed service||C57BL/6||$3,950||6-16 weeks|
|BAC injection to obtain founders, basic service*||C57BL/6||$4,450||8-14 weeks|
|BAC injection to obtain founders, guaranteed service*||C57BL/6||$6,950||8-18 weeks|
|Genotyping pups to identify founders||$250||1 week|
We typically produce transgenic mice in C57BL/6 strain backgrounds, but we can use other strains per your request.
>> Rat models are also available. Learn more about Regular Transgenic Rat Services.
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.
Please see below for supporting information related to Cyagen animal orders.