huANGPTL3 Mouse
Product Name
huANGPTL3 Mouse
Product ID
C001400
Strain Name
C57BL/6JCya-Angptl3tm1(hANGPTL3)/Cya
Background
C57BL/6JCya
Status
Citations
0
When using this mouse strain in a publication, please cite “huANGPTL3 Mouse (Catalog C001400) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
ANGPTL3
Gene Alias
ANL3, ANG-5, FHBL2, ANGPT5
NCBI ID
Chromosome
Chr 1 (Human)
MGI ID
Datasheet
Strain Description
Angiopoietin‑like protein 3 (ANGPTL3) is mainly expressed in the liver and is a secreted glycoprotein structurally related to angiopoietins. The mature form of human ANGPTL3 protein contains an N‑terminal coiled‑coil domain and a C‑terminal fibrinogen‑like (FBN) domain. Through binding of its FBN‑like domain to integrin αvβ3, ANGPTL3 induces endothelial cell adhesion and migration, thereby playing a role in the regulation of angiogenesis [1]. In addition, ANGPTL3 can directly inhibit lipoprotein lipase (LPL) and endothelial lipase (EL), which are closely involved in the hydrolysis of circulating triglycerides (TG) and high‑density lipoprotein cholesterol (HDL‑C), respectively, thereby elevating circulating TG levels and affecting HDL levels [2-3]. In humans, ANGPTL3 is an important factor for HDL levels, and its plasma level is positively correlated with HDL‑C. Loss‑of‑function mutations in the ANGPTL3 gene (especially homozygous or compound heterozygous) lead to familial combined hypolipidemia (FHBL2) [4]. Furthermore, ANGPTL3 also plays important roles in biological and pathological processes related to lipid metabolism and angiogenesis, such as atherosclerosis, tumors, nephrotic syndrome, diabetes, and liver disease [3].
This strain is a mouse Angptl3 gene humanized model. The mouse Angptl3 gene is replaced with the human ANGPTL3 gene sequence by gene editing technology, which expresses the human ANGPTL3 protein while retaining the signal peptide of mouse Angptl3. This model can be used to study the pathogenesis of metabolic diseases such as atherosclerosis, diabetes, and familial combined hyperlipidemia (FCH), as well as the development and screening of ANGPTL3-targeted drugs. The homozygous huANGPTL3 mice are viable and fertile.
Reference
Camenisch G, Pisabarro MT, Sherman D, et al. ANGPTL3 stimulates endothelial cell adhesion and migration via integrin αvβ3 and induces blood vessel formation in vivo. J Biol Chem. 2002;277(19):17281-17290.
Shimamura M, Matsuda M, Yasumo H, et al. Angiopoietin-like protein3 regulates plasma HDL cholesterol through suppression of endothelial lipase. Arterioscler Thromb Vasc Biol. 2007;27(2):366-372.
Kersten S. Angiopoietin-like 3 in lipoprotein metabolism. Nat Rev Endocrinol. 2017;13(12):731-744.
Musunuru K, Pirruccello JP, Do R, Peloso GM, Guiducci C, Sougnez C, Garimella KV, Fisher S, Abreu J, Barry AJ, Fennell T, Banks E, Ambrogio L, Cibulskis K, Kernytsky A, Gonzalez E, Rudzicz N, Engert JC, DePristo MA, Daly MJ, Cohen JC, Hobbs HH, Altshuler D, Schonfeld G, Gabriel SB, Yue P, Kathiresan S. Exome sequencing, ANGPTL3 mutations, and familial combined hypolipidemia. N Engl J Med. 2010 Dec 2;363(23):2220-7.
Watts GF, Scott R, Sullivan D, et al. Zodasiran for cholesterol and triglyceride lowering in patients with hyperlipidemia: final report of phase 1 basket trial. Nat Med. 2026;32(4):1432-1443.
Strain Strategy
The mouse endogenous region from p.S17 to downstream of Exon 7 was replaced with the human ANGPTL3 region from p.S17 to downstream of Exon 7. The murine signal peptide remained.
Figure 1. The gene editing strategy for generating huANGPTL3 mice.
Application Area
Metabolic diseases such as atherosclerosis and hyperlipidemia;
Angiogenesis and endothelial cell adhesion;
Development and screening of ANGPTL3-targeted drugs.
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