huPD-1 Mouse
Product Name
huPD-1 Mouse
Product ID
C001524
Strain Name
C57BL/6JCya-Pdcd1em1(hPDCD1)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “huPD-1 Mouse (Catalog C001524) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
PDCD1
Gene Alias
PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1
NCBI ID
Chromosome
Chr 2 (Human)
MGI ID
Datasheet
Strain Description
Programmed cell death protein 1 (PDCD1/PD-1) is a member of the B7-CD28 costimulatory receptor family. It is an inhibitory receptor expressed on activated T cells and plays a role in regulating the function of effector T cells, including CD8+ T cells, and promoting the differentiation of CD4+ T cells into regulatory T cells. PD-1 is expressed in a variety of tumors and plays an important role in antitumor immunity. In addition, PD-1 is involved in the defense against autoimmune diseases and has inhibitory effects on antitumor and antimicrobial immunity [1]. PD-1 binds to programmed death ligands 1 and 2 (PD-L1 and PD-L2) to inhibit T cell activation, reduce the production of corresponding cytokines, and regulate T cell survival [2]. Drugs targeting this pathway can reactivate T cells to activate antitumor immune responses [3].
This strain is a genetically modified mouse model in which the gene encoding the mouse PD-1 protein is humanized, resulting in the expression of human PD-1 protein in mice. This model can be used for research in drug development, efficacy and safety evaluation, tumor immunotherapy evaluation, and immune system mechanisms related to PD-1.
Reference
National Center for Biotechnology Information. "Gene: PDCD1." NCBI, U.S. National Library of Medicine, 2023, www.ncbi.nlm.nih.gov/gene/5133
Okazaki T, Honjo T. PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 2007 Jul;19(7):813-24.
Sharpe AH, Pauken KE. The diverse functions of the PD1 inhibitory pathway. Nat Rev Immunol. 2018 Mar;18(3):153-167.
Kwok G, Yau TC, Chiu JW, Tse E, Kwong YL. Pembrolizumab (Keytruda). Hum Vaccin Immunother. 2016 Nov;12(11):2777-2789.
Strain Strategy
The mouse Pdcd1 gene was edited using gene editing technology to replace the sequence encoding the extracellular domain of mouse PD-1 protein with the sequence from the human PD1 gene encoding the human PD-1 protein extracellular domain while retaining the mouse signal peptide.
Figure 1. Gene editing strategy for huPD-1 mice.
Application Area
Development and screening of PD-1-targeted drugs;
Efficacy and safety evaluation of PD-1-targeted drugs;
Evaluation of tumor immunotherapy;
Research on the immune system and tumor immune escape mechanisms.
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