ES Cell Gene Targeting
Cyagen’s ES Cell Gene Targeting platform leverages homologous recombination in murine embryonic stem (ES) cells to deliver highly precise, complex gene modifications. It is ideal for conditional knockouts, reporter knockins, humanized models, and large-fragment insertions.
No Off-Target Concerns
Ideal for sensitive applications with zero nuclease-related off-target risks.
Complex Edits Made Easy
Enables large-fragment knockins and humanization—up to ~20 kb or more.
Trusted Precision
Decades-proven ES cell targeting ensures accurate and reliable gene edits.
Technology
Applications
FAQs
How ES Cell Gene Targeting Works
ES Cell Gene Targeting introduces specific genetic modifications by
homologous recombination within embryonic stem (ES) cells. The
engineered ES cells are then injected into blastocysts to generate
chimeric mice, which transmit the desired allele through the germline.
While this traditional method requires longer timelines compared to
newer editing technologies, it remains uniquely suited for complex
edits, especially those involving large DNA fragments or sophisticated
genetic constructs.
| Feature | ES Cell Gene Targeting | TurboKnockout® (Enhanced) | Targeted Gene Editing |
|---|---|---|---|
| Turnaround Time | 10-14 months | 6-8 months | 5-7 months |
| Approach | ES Cell-mediated HR | Proprietary ESC + self-deleting cassette | Embryo-stage nuclease targeting |
| Max Knockin Size | ~20 kb (RMCE: ~300 kb) | ~20 kb (RMCE: ~300 kb) | ≤15 kb |
| Off-target Risk | None | None | Moderate |
| Screening Method | PCR + Southern Blot | PCR + Southern Blot | PCR + Sequencing |
| Applications | KO, cKO, Point Mutation, KI, Humanization | KO, cKO, KI, Humanization | KO, cKO, Point Mutation |
| Patent Risk | Low | None | High (restricted regions) |
Looking for a faster and more efficient alternative?
Explore TurboKnockout® – Our Next-Generation ES
Cell Targeting Technology →
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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