Custom Models
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- ● Diet-induced obesity (DIO) Model
- ● Type 1 Diabetes Mellitus (T1DM) Mouse Model
- ● Type 2 Diabetes Mellitus (T2DM) Mouse Model
- ● Diet-induced Non-alcoholic Fatty Liver Disease (NAFLD) Mouse Model
- ● Chemically Induced Non-alcoholic Fatty Liver Disease (NAFLD) Mouse Model
- ● Non-alcoholic Fatty Liver Disease (NAFLD) Mouse Model (Gene-editing)
- ● Composite (Combined) Model - Non-alcoholic Fatty Liver Disease (NAFLD) Model
- ● Composite Model - Atherosclerosis Mouse Model
- ● Atherosclerosis Mouse Model (Gene-editing)
- ● Acute Pancreatitis Mouse Model
- ● Chronic Pancreatitis Mouse Model
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Testimonials
"I've been very happy with Cyagen's service so I've been referring a lot of colleagues... thanks for
the
great service."
'- Washington University in St. Louis
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Citations
IMMUNITY 47:107 (2017) IF=22.845
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Transgenic services
>> Taconic-Cyagen
Model Generation Alliance
We provide a full catalog of expert model generation services with guaranteed genotype and multiple import options that mitigate quarantine.
» Request a Quote or InformationCyagen provides comprehensive model generation services for all your transgenic mouse, mouse embryo, and rat model needs. Contact us to perform your entire project - from initial strategy design and DNA vector construction, all the way through breeding – we deliver research-ready transgenic rodent models for guaranteed results.
Cyagen’s Transgenic Rodent Model Generation Capabilities
| Regular Transgenic | PiggyBac Transgenic | ROSA26 Large-Fragment Knockin | |
|---|---|---|---|
| Integration | Random, multicopy integration | Random, single copy per integration site | Single copy transgene targeted to ROSA26 safe harbor locus |
| Vector construction | Transgenic plasmid or BAC | Transgenic plasmid or BAC | Targeting vector + gRNAs |
| Expression pattern | Variable expression in founders | More consistent expression in founders | Most consistent expression |
| Zygosity | Hemizygous | Hemizygous | Options: Heterozygous and Homo |
| Endogenous effects | Can disrupt endogenous gene expression | Less likely to disrupt endogenous gene expression | Safe harbor site (SHS) does not disrupt endogenous gene expression |
| Available Services | |||
| Turnaround time | 2-5 months | 6-9 months | |
| Donor background | Mouse strains: C57BL/6, FVB Rat strains: Sprague-Dawley (SD), Long Evans Please inquire for the availability of additional strains. |
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Advantages of PiggyBac Transgenic Services
Our proprietary PiggyBac transgenic method has the following advantages over other transgenic approaches:
- Single-copy integration: Avoids potential gene silencing from multiple copies per integration site
- Defined region of integration: No loss of transgene sequence (TTAA, transcription unit)
- Reliability: More consistent expression pattern compared to plasmid-based transgenics
- Economical: Cost and turnaround time comparable to plasmid-based transgenics
Why work with Cyagen?
- ● 14+ years' experience in custom animal model generation
- ● 78,000+ animal models generated and delivered worldwide
- ● 4,700+ citations in SCI journals
- ● 3,000+ university and company partners
- ● 100% money-back guarantee option available for most services
- ● AAALAC-Accredited and OLAW Assured
