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Cyagen provides comprehensive model generation services for all your transgenic mouse, mouse embryo, and rat model needs. Contact us to perform your entire project - from initial strategy design and DNA vector construction, all the way through breeding – we deliver research-ready transgenic rodent models for guaranteed results.
Regular Transgenic | PiggyBac Transgenic | ROSA26 Large-Fragment Knockin | |
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Integration | Random, multicopy integration | Random, single copy per integration site | Single copy transgene targeted to ROSA26 safe harbor locus |
Vector construction | Transgenic plasmid or BAC | Transgenic plasmid or BAC | Targeting vector + gRNAs |
Expression pattern | Variable expression in founders | More consistent expression in founders | Most consistent expression |
Zygosity | Hemizygous | Hemizygous | Options: Heterozygous and Homo |
Endogenous effects | Can disrupt endogenous gene expression | Less likely to disrupt endogenous gene expression | Safe harbor site (SHS) does not disrupt endogenous gene expression |
Available Services | |||
Turnaround time | 2-5 months | 6-9 months | |
Donor background | Mouse strains: C57BL/6, FVB Rat strains: Sprague-Dawley (SD), Long Evans Please inquire for the availability of additional strains. |
Our proprietary PiggyBac transgenic method has the following advantages over other transgenic approaches: