Transgenic services

 

Cyagen provides comprehensive model generation services for all your transgenic mouse, mouse embryo, and rat model needs. Contact us to perform your entire project - from initial strategy design and DNA vector construction, all the way through breeding – we deliver research-ready transgenic rodent models for guaranteed results.

 

 

Cyagen’s Transgenic Rodent Model Generation Capabilities

  Regular Transgenic PiggyBac Transgenic ROSA26 Large-Fragment Knockin
Integration Random, multicopy integration Random, single copy per integration site Single copy transgene targeted to ROSA26 safe harbor locus
Vector construction Transgenic plasmid or BAC Transgenic plasmid or BAC Targeting vector + gRNAs
Expression pattern Variable expression in founders More consistent expression in founders Most consistent expression
Zygosity Hemizygous Hemizygous Options: Heterozygous and Homo
Endogenous effects Can disrupt endogenous gene expression Less likely to disrupt endogenous gene expression Safe harbor site (SHS) does not disrupt endogenous gene expression
Available Services
Turnaround time 2-5 months 6-9 months
Donor background Mouse strains: C57BL/6, FVB
Rat strains: Sprague-Dawley (SD), Long Evans
Please inquire for the availability of additional strains.

  

Advantages of PiggyBac Transgenic Services

Our proprietary PiggyBac transgenic method has the following advantages over other transgenic approaches:

  • Single-copy integration: Avoids potential gene silencing from multiple copies per integration site
  • Defined region of integration: No loss of transgene sequence (TTAA, transcription unit)
  • Reliability: More consistent expression pattern compared to plasmid-based transgenics
  • Economical: Cost and turnaround time comparable to plasmid-based transgenics

 

Why work with Cyagen?

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,700+ citations in SCI journals
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured