The Ube3a KO mouse is a gene knockout (KO) model, generated using gene editing technology to knock out the protein-coding sequence of the Ube3a gene (the homologous gene of human UBE3A gene) in mice. Preliminary behavioral data indicate that this model exhibits anxiety-like/compulsive behaviors, abnormal stress responses, and is accompanied by decreased spontaneous activity, shortened movement distance, and reduced average motility, among other motor function and behavioral abnormalities. It can be used for research on the pathogenesis of Angelman Syndrome (AS) and the development of related therapies.