C57BL/6JCya-Prdm1em1flox/Cya
Common Name
Prdm1-flox
Product ID
S-CKO-01425
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12142-Prdm1-B6J-VA
When using this mouse strain in a publication, please cite “Prdm1-flox Mouse (Catalog S-CKO-01425) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Prdm1-flox
Strain ID
CKOCMP-12142-Prdm1-B6J-VA
Gene Name
Product ID
S-CKO-01425
Gene Alias
Blimp1, Blimp-1, PRDI-BF1, ZNFPR1A1, b2b1765Clo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000105490
NCBI RefSeq
NM_007548
Target Region
Exon 3~4
Size of Effective Region
~4.6 kb
Overview of Gene Research
Prdm1, also known as BLIMP1, is a key transcription factor. It plays important roles in cell differentiation, immune regulation, and cancer-related processes. In B cells, it acts as a master regulator of plasma cell differentiation; in T cells, it is crucial for terminal effector cell differentiation [2]. It is also involved in pathways related to cancer immune evasion and T-cell hyporesponsiveness. Genetic models, especially KO/CKO mouse models, are valuable for studying Prdm1.
In a Ncr1-driven conditional knockout mouse model, Prdm1 was shown to regulate the composition and maturation of cNK cells in the liver. Prdm1-deficient cNK cells and liver ILC1s had decreased secretion of interferon-γ, granzyme B, and perforin, leading to increased cancer metastasis [3]. Genetic knockout of Prdm1 in antitumor T cells by CRISPR/Cas9 supported the maintenance of an early memory phenotype and polyfunctional cytokine secretion in repeatedly stimulated CAR-engineered T cells, enhancing T-cell persistence and therapeutic efficacy in multiple tumor models [1].
In conclusion, Prdm1 is essential for cell differentiation in B and T cells and significantly impacts immune-related processes. Studies using KO/CKO mouse models have revealed its role in cancer metastasis, T-cell-based immunotherapy, and immune surveillance, providing insights into potential therapeutic strategies for liver cancer and adoptive cancer immunotherapies.
References：
1. Yoshikawa, Toshiaki, Wu, Zhiwen, Inoue, Satoshi, Suzuki, Shiro, Kagoya, Yuki. . Genetic ablation of PRDM1 in antitumor T cells enhances therapeutic efficacy of adoptive immunotherapy. In Blood, 139, 2156-2172. doi:10.1182/blood.2021012714. https://pubmed.ncbi.nlm.nih.gov/34861037/
2. Boi, Michela, Zucca, Emanuele, Inghirami, Giorgio, Bertoni, Francesco. 2014. PRDM1/BLIMP1: a tumor suppressor gene in B and T cell lymphomas. In Leukemia & lymphoma, 56, 1223-8. doi:10.3109/10428194.2014.953155. https://pubmed.ncbi.nlm.nih.gov/25115512/
3. He, Jitian, Gao, Le, Wang, Peiying, Yang, Zhouxin, Wang, Youwei. 2024. Prdm1 positively regulates liver Group 1 ILCs cancer immune surveillance and preserves functional heterogeneity. In eLife, 13, . doi:10.7554/eLife.92948. https://pubmed.ncbi.nlm.nih.gov/39133873/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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