C57BL/6JCya-Btg2em1flox/Cya
Common Name
Btg2-flox
Product ID
S-CKO-01462
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12227-Btg2-B6J-VA
When using this mouse strain in a publication, please cite “Btg2-flox Mouse (Catalog S-CKO-01462) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Btg2-flox
Strain ID
CKOCMP-12227-Btg2-B6J-VA
Gene Name
Product ID
S-CKO-01462
Gene Alias
Pc3, APRO1, TIS21
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020692
NCBI RefSeq
NM_007570
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Overview of Gene Research
Btg2, also known as PC3 or TIS21, belongs to the antiproliferative (APRO) gene family [3]. It acts as a tumor suppressor and is involved in numerous biological activities such as cell differentiation, proliferation, DNA damage repair, and apoptosis [3]. It is an immediate early response gene regulated by various factors and signaling pathways [3]. Also, it has been found to be related to maintaining T cell quiescence by promoting mRNA deadenylation and degradation [2].
In FSGS, podocyte-specific deletion of Btg2 protected against the onset of proteinuria and glomerulosclerosis in ADR-treated mice, inhibiting EMT markers and restoring epithelial markers [1]. This indicates Btg2 promotes FSGS via Smad3-dependent podocyte-mesenchymal transition [1]. In muscle stem cells (MuSCs), overexpression and knockdown of Btg2 in primary MuSCs respectively stimulated and prevented MuSCs senescence, suggesting Btg2 is a regulator of MuSC senescence [4].
In conclusion, Btg2 is crucial in multiple biological processes. Gene-knockout (KO) or conditional-knockout (CKO) mouse models have been instrumental in revealing its role in diseases like FSGS and muscle aging. These models help us understand that Btg2 can act as a regulator in disease-related cellular processes, providing potential therapeutic targets for these conditions.
References：
1. Dan Hu, Qiong-, Wang, Hong-Lian, Liu, Jian, Lan, Hui-Yao, Wang, Li. 2023. Btg2 Promotes Focal Segmental Glomerulosclerosis via Smad3-Dependent Podocyte-Mesenchymal Transition. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2304360. doi:10.1002/advs.202304360. https://pubmed.ncbi.nlm.nih.gov/37749872/
2. Hwang, Soo Seok, Lim, Jaechul, Yu, Zhibin, Li, Hua-Bing, Flavell, Richard A. . mRNA destabilization by BTG1 and BTG2 maintains T cell quiescence. In Science (New York, N.Y.), 367, 1255-1260. doi:10.1126/science.aax0194. https://pubmed.ncbi.nlm.nih.gov/32165587/
3. Mao, Bijing, Zhang, Zhimin, Wang, Ge. 2014. BTG2: a rising star of tumor suppressors (review). In International journal of oncology, 46, 459-64. doi:10.3892/ijo.2014.2765. https://pubmed.ncbi.nlm.nih.gov/25405282/
4. Peng, Baozhou, Chen, Yihan, Wang, Yaning, Wang, Shuaiyu, Zhang, Hongbo. 2023. BTG2 acts as an inducer of muscle stem cell senescence. In Biochemical and biophysical research communications, 669, 113-119. doi:10.1016/j.bbrc.2023.05.098. https://pubmed.ncbi.nlm.nih.gov/37269593/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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