C57BL/6JCya-Bub1bem1flox/Cya
Common Name:
Bub1b-flox
Product ID:
S-CKO-01467
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bub1b-flox
Strain ID
CKOCMP-12236-Bub1b-B6J-VA
Gene Name
Product ID
S-CKO-01467
Gene Alias
BUBR1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bub1bem1flox/Cya mice (Catalog S-CKO-01467) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038341
NCBI RefSeq
NM_009773
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
BUB1b, also known as BUB1 mitotic checkpoint serine/threonine kinase B, is a crucial gene involved in the mitotic spindle assembly checkpoint. It plays a key role in ensuring accurate chromosome segregation during cell division, which is essential for maintaining genomic stability. Dysregulation of BUB1b can lead to chromosomal instability, a hallmark of many cancers [3,4].
In multiple cancers, loss-of-function assays have revealed its oncogenic role. In lung adenocarcinoma, knockdown of BUB1b reduced cell viability, and it was found to enhance resistance to ferroptosis and chemotherapy by stabilizing the NRF2 signaling pathway through forming a complex with OTUD3 and NRF2 [1]. In extrahepatic cholangiocarcinoma, inhibition of BUB1b decreased cell proliferation and invasiveness both in vitro and in vivo, and it was shown to act via the JNK/c-Jun pathways [2]. Similar results were seen in multiple myeloma, where targeting BUB1B abrogated cellular proliferation and induced drug sensitivity [4]. In gastric cancer, BUB1B knockdown hampered DNA repair and increased death in cisplatin-resistant cells, suggesting its role in promoting cisplatin resistance via Rad51-mediated DNA damage repair [5].
In conclusion, BUB1b is essential for proper cell division and its dysregulation contributes significantly to cancer progression. Studies using loss-of-function models across various cancers, such as lung adenocarcinoma, extrahepatic cholangiocarcinoma, multiple myeloma, and gastric cancer, have highlighted its oncogenic potential and its role in chemotherapy resistance. These findings suggest BUB1b could be a potential therapeutic target in these cancer types.
References:
1. Ding, Yanguang, Gao, Jian, Chen, Jun, Tong, Xin, Zhao, Jun. 2024. BUB1b impairs chemotherapy sensitivity via resistance to ferroptosis in lung adenocarcinoma. In Cell death & disease, 15, 525. doi:10.1038/s41419-024-06914-0. https://pubmed.ncbi.nlm.nih.gov/39043653/
2. Jiao, Chen Yu, Feng, Qin Chao, Li, Chang Xian, Wang, Xuehao, Li, Xiang Cheng. 2021. BUB1B promotes extrahepatic cholangiocarcinoma progression via JNK/c-Jun pathways. In Cell death & disease, 12, 63. doi:10.1038/s41419-020-03234-x. https://pubmed.ncbi.nlm.nih.gov/33431813/
3. Silva, Maria P, Ferreira, Luísa T, Brás, Natércia F, Teixeira, Manuel R, Paulo, Paula. 2024. BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability. In Journal of biomedical science, 31, 74. doi:10.1186/s12929-024-01056-z. https://pubmed.ncbi.nlm.nih.gov/39014450/
4. Tang, Xiaozhu, Guo, Mengjie, Ding, Pinggang, Gu, Xiaosong, Yang, Ye. 2021. BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability. In Signal transduction and targeted therapy, 6, 361. doi:10.1038/s41392-021-00746-6. https://pubmed.ncbi.nlm.nih.gov/34620840/
5. Qin, Zhe, Ye, Fangzhou, Wang, Jiayi, Li, Huanqing, Feng, Li. 2025. BUB1B promotes cisplatin resistance in gastric cancer via Rad51-mediated DNA damage repair. In Translational oncology, 54, 102334. doi:10.1016/j.tranon.2025.102334. https://pubmed.ncbi.nlm.nih.gov/40056529/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen