C57BL/6JCya-Ccr6em1flox/Cya
Common Name:
Ccr6-flox
Product ID:
S-CKO-01598
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ccr6-flox
Strain ID
CKOCMP-12458-Ccr6-B6J-VA
Gene Name
Product ID
S-CKO-01598
Gene Alias
CC-CKR-6; CCR-6; Cmkbr6; KY411
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccr6em1flox/Cya mice (Catalog S-CKO-01598) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000097418
NCBI RefSeq
NM_009835
Target Region
Exon 5~6
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Ccr6, a G protein-coupled receptor, is expressed on various cells such as B cells, immature dendritic cells, innate lymphoid cells, regulatory CD4 T cells, and Th17 cells [1,3,6,9]. It binds to its sole ligand CCL20, forming a signaling network that is implicated in multiple biological processes including immune homeostasis, activation, and leukocyte migration during inflammation [1,2,3,5,6,7,8,9]. This CCR6-CCL20 axis is crucial in various diseases like autoimmune diseases, cancers, and inflammatory diseases [1,2,3,5].
In colitis-associated carcinoma, loss of SMAD4 in mouse colon epithelium leads to increased CCL20 expression and recruitment of CCR6+ immune cells such as T regulatory, Th17, and dendritic cells, contributing to tumorigenesis. However, loss of CCR6 abrogates these immune responses and reduces the incidence of colitis-associated tumors [4]. In psoriasis, IL-17A-producing immune cells stimulate epidermal keratinocytes to produce CCL20, which recruits CCR6+ Th17 cells into lesional skin, creating an IL-17A-rich cutaneous milieu [5]. In human chronic pancreatitis, single-cell sequencing shows distinct immune characteristics with significantly enriched CCR6+ CD4+ T cells and up-regulation of CCL20 among monocytes in hereditary CP, suggesting the CCR6-CCL20 axis as a potential target [7].
In conclusion, Ccr6, through its interaction with CCL20, plays essential roles in immune-related biological processes and is involved in the pathogenesis of multiple diseases. Gene knockout models, such as the CCR6 knockout mice, have revealed its significance in colitis-associated carcinoma, psoriasis, and chronic pancreatitis, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Meitei, Heikrujam Thoihen, Jadhav, Nandadeep, Lal, Girdhari. 2021. CCR6-CCL20 axis as a therapeutic target for autoimmune diseases. In Autoimmunity reviews, 20, 102846. doi:10.1016/j.autrev.2021.102846. https://pubmed.ncbi.nlm.nih.gov/33971346/
2. Kadomoto, Suguru, Izumi, Kouji, Mizokami, Atsushi. 2020. The CCL20-CCR6 Axis in Cancer Progression. In International journal of molecular sciences, 21, . doi:10.3390/ijms21155186. https://pubmed.ncbi.nlm.nih.gov/32707869/
3. Gómez-Melero, Sara, Caballero-Villarraso, Javier. 2023. CCR6 as a Potential Target for Therapeutic Antibodies for the Treatment of Inflammatory Diseases. In Antibodies (Basel, Switzerland), 12, . doi:10.3390/antib12020030. https://pubmed.ncbi.nlm.nih.gov/37092451/
4. Hanna, David N, Smith, Paula Marincola, Novitskiy, Sergey V, Beauchamp, R Daniel, Means, Anna L. 2022. SMAD4 Suppresses Colitis-associated Carcinoma Through Inhibition of CCL20/CCR6-mediated Inflammation. In Gastroenterology, 163, 1334-1350.e14. doi:10.1053/j.gastro.2022.07.016. https://pubmed.ncbi.nlm.nih.gov/35863523/
5. Furue, Kazuhisa, Ito, Takamichi, Tsuji, Gaku, Nakahara, Takeshi, Furue, Masutaka. 2019. The CCL20 and CCR6 axis in psoriasis. In Scandinavian journal of immunology, 91, e12846. doi:10.1111/sji.12846. https://pubmed.ncbi.nlm.nih.gov/31692008/
6. Lee, Adrian Y S, Körner, Heinrich. 2019. The CCR6-CCL20 axis in humoral immunity and T-B cell immunobiology. In Immunobiology, 224, 449-454. doi:10.1016/j.imbio.2019.01.005. https://pubmed.ncbi.nlm.nih.gov/30772094/
7. Lee, Bomi, Namkoong, Hong, Yang, Yan, Bellin, Melena D, Habtezion, Aida. 2021. Single-cell sequencing unveils distinct immune microenvironments with CCR6-CCL20 crosstalk in human chronic pancreatitis. In Gut, 71, 1831-1842. doi:10.1136/gutjnl-2021-324546. https://pubmed.ncbi.nlm.nih.gov/34702715/
8. Lee, Adrian Y S, Körner, Heinrich. 2017. CCR6/CCL20 chemokine axis in human immunodeficiency virus immunity and pathogenesis. In The Journal of general virology, 98, 338-344. doi:10.1099/jgv.0.000691. https://pubmed.ncbi.nlm.nih.gov/28005525/
9. Williams, Ifor R. . CCR6 and CCL20: partners in intestinal immunity and lymphorganogenesis. In Annals of the New York Academy of Sciences, 1072, 52-61. doi:. https://pubmed.ncbi.nlm.nih.gov/17057190/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen