C57BL/6JCya-Cd34em1flox/Cya
Common Name:
Cd34-flox
Product ID:
S-CKO-01617
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cd34-flox
Strain ID
CKOCMP-12490-Cd34-B6J-VA
Gene Name
Product ID
S-CKO-01617
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cd34em1flox/Cya mice (Catalog S-CKO-01617) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016638
NCBI RefSeq
NM_133654
Target Region
Exon 2~3
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Cd34 is a cell surface antigen expressed in numerous stem/progenitor cells, such as hematopoietic stem cells (HSCs) and endothelial progenitor cells (EPCs) [1]. These CD34+ cells are involved in angiogenesis, anti-inflammatory, immunomodulatory, and anti-apoptosis/fibrosis roles through paracrine activity, and also contribute to direct incorporation into the expanding vasculature [1].
In heart-related studies, in murine pressure overload models (transverse aortic constriction, TAC), non-bone-marrow-derived CD34+ cells give rise to fibroblasts and endothelial cells, while bone-marrow-derived CD34+ cells turn into immune cells in response to pressure overload. Partial depletion of CD34+ cells in Cd34-CreERT2; Rosa26-eGFP-DTA (Cre/DTA) mice alleviated myocardial fibrosis and improved cardiac function, suggesting a role in heart failure-related fibrosis [2]. Similar findings were observed in ischemia/reperfusion (I/R) injury models, where depletion of Cd34+ cells using a Cd34-CreERT2; R26-DTA mouse model alleviated ventricular fibrosis and improved cardiac function, with Cd34+ cells contributing to mesenchymal cell, endothelial cell, and monocyte/macrophage commitment during heart remodeling [3].
In conclusion, Cd34+ cells play crucial roles in processes like angiogenesis and tissue repair. Studies using gene-knockout (KO) or conditional-knockout (CKO) mouse models have revealed their significance in heart-related diseases, especially in myocardial fibrosis and cardiac remodeling after injury, providing potential therapeutic implications for heart failure treatment.
References：
1. Hassanpour, Mehdi, Salybekov, Amankeldi A, Kobayashi, Shuzo, Asahara, Takayuki. 2023. CD34 positive cells as endothelial progenitor cells in biology and medicine. In Frontiers in cell and developmental biology, 11, 1128134. doi:10.3389/fcell.2023.1128134. https://pubmed.ncbi.nlm.nih.gov/37138792/
2. Du, Luping, Sun, Xiaotong, Gong, Hui, Chen, Ting, Xu, Qingbo. 2023. Single cell and lineage tracing studies reveal the impact of CD34+ cells on myocardial fibrosis during heart failure. In Stem cell research & therapy, 14, 33. doi:10.1186/s13287-023-03256-0. https://pubmed.ncbi.nlm.nih.gov/36805782/
3. Xie, Jun, Jiang, Liujun, Wang, Junzhuo, Xu, Biao, Xu, Qingbo. 2023. Multilineage contribution of CD34+ cells in cardiac remodeling after ischemia/reperfusion injury. In Basic research in cardiology, 118, 17. doi:10.1007/s00395-023-00981-8. https://pubmed.ncbi.nlm.nih.gov/37147443/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen