C57BL/6JCya-Cd86em1flox/Cya
Common Name:
Cd86-flox
Product ID:
S-CKO-01645
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Cd86-flox
Strain ID
CKOCMP-12524-Cd86-B6J-VA
Gene Name
Product ID
S-CKO-01645
Gene Alias
B7; B7-2; B7.2; B70; CLS1; Cd28l2; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cd86em1flox/Cya mice (Catalog S-CKO-01645) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089620
NCBI RefSeq
NM_019388
Target Region
Exon 3~4
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Cd86, also known as B7-2, is a costimulatory molecule that plays a crucial role in the immune system. It interacts with CD28 and CTLA-4 present on T cells, which is essential for the full activation of naïve T-cells and their subsequent differentiation. This interaction is involved in pathways regulating T cell immunity, balancing activation and inhibition of T cell responses [1,2,3,4].
CTLA-4 can capture CD86 from antigen-presenting cells via trans-endocytosis, leading to the degradation of this costimulatory ligand and impaired costimulation via CD28 [2]. Mutations that disrupt CD86 trans-endocytosis can cause autoimmune diseases, indicating a link between defects in this process and autoimmunity [1].
In the context of regulatory T cells (Tregs), CD86 is the dominant ligand for Treg proliferation, survival, and maintenance of a regulatory phenotype, despite its lower affinity for CD28, as CD80-CD28 interactions are selectively impaired by high levels of CTLA-4 [6].
Low CD86 expression in exfoliated cervical cells can predict histological complete response after administration of the therapeutic human papillomavirus vaccine IGMKK16E7 [5].
In conclusion, Cd86 is essential for T cell-mediated immune responses. Research on Cd86, especially through models like CTLA-4-mediated trans-endocytosis studies, has provided insights into its role in autoimmunity and as a biomarker in vaccine response. Understanding Cd86 is crucial for comprehending immune regulation and developing treatments related to immune-mediated diseases and vaccines.
References:
1. Kennedy, Alan, Waters, Erin, Rowshanravan, Behzad, Walker, Lucy S K, Sansom, David M. 2022. Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation. In Nature immunology, 23, 1365-1378. doi:10.1038/s41590-022-01289-w. https://pubmed.ncbi.nlm.nih.gov/35999394/
2. Qureshi, Omar S, Zheng, Yong, Nakamura, Kyoko, Walker, Lucy S K, Sansom, David M. 2011. Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4. In Science (New York, N.Y.), 332, 600-3. doi:10.1126/science.1202947. https://pubmed.ncbi.nlm.nih.gov/21474713/
3. Van Coillie, Samya, Wiernicki, Bartosz, Xu, Jie. . Molecular and Cellular Functions of CTLA-4. In Advances in experimental medicine and biology, 1248, 7-32. doi:10.1007/978-981-15-3266-5_2. https://pubmed.ncbi.nlm.nih.gov/32185705/
4. Pereira, Celso, Tavares, Beatriz, Loureiro, Graça, Botelho, Filomena, Baganha, Manuel F. . Dynamics of CD86 expression on allergic inflammation--new insights. In Recent patents on inflammation & allergy drug discovery, 3, 128-31. doi:. https://pubmed.ncbi.nlm.nih.gov/19519589/
5. Ando, Hanano, Katoh, Yuki, Kobayashi, Osamu, Schust, Danny J, Kawana, Kei. . Low CD86 expression is a predictive biomarker for clinical response to the therapeutic human papillomavirus vaccine IGMKK16E7: results of a post hoc analysis. In JNCI cancer spectrum, 8, . doi:10.1093/jncics/pkae091. https://pubmed.ncbi.nlm.nih.gov/39302712/
6. Halliday, Neil, Williams, Cayman, Kennedy, Alan, Walker, Lucy S K, Sansom, David M. 2020. CD86 Is a Selective CD28 Ligand Supporting FoxP3+ Regulatory T Cell Homeostasis in the Presence of High Levels of CTLA-4. In Frontiers in immunology, 11, 600000. doi:10.3389/fimmu.2020.600000. https://pubmed.ncbi.nlm.nih.gov/33363541/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen