C57BL/6JCya-Ccr5em1flox/Cya
Common Name:
Ccr5-flox
Product ID:
S-CKO-01773
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccr5-flox
Strain ID
CKOCMP-12774-Ccr5-B6J-VA
Gene Name
Product ID
S-CKO-01773
Gene Alias
AM4-7; CD195; Cmkbr5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccr5em1flox/Cya mice (Catalog S-CKO-01773) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111442
NCBI RefSeq
NM_009917
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Ccr5, a cell membrane protein from the G protein-coupled receptors (GPCR) family, is a crucial modulator for leukocyte activation and mobilization. It is best known as an important coreceptor used by HIV to infect target cells [1,5,6,8]. Additionally, it is involved in the chemokine system, which mediates acute inflammation by guiding leukocyte migration to damaged or infected tissues [8].
Genetic deficiency of Ccr5 confers a significant degree of protection against HIV infection, and since this deficiency does not seem to cause adverse health effects, targeting Ccr5 is a promising strategy for HIV treatment and prevention [1]. In metastatic colorectal cancer, the CCR/L5 axis (including Ccr5) has dichotomous functions in cancer, influencing both tumor progression and immune responses. Low expression of this axis was correlated with improved survival, especially in patients treated with chemotherapy plus cetuximab [2]. Ccr5 promotes immune responses by recruiting immune cells to the sites of inflammation/tumor, and also contributes to an immune-suppressive tumor microenvironment, thus recognized as a potential therapeutic target for cancer [3]. In paclitaxel-induced peripheral neuropathy, blockade of Ccr5 suppresses the development of neuropathic nociception, suggesting it as a target for treatment [4]. In pulmonary ischemia-reperfusion injury, Ccr5 drives NK cell-associated airway damage, and its blockade could be a preventative treatment [7].
In summary, Ccr5 is essential in multiple biological processes and disease conditions. Its role in facilitating HIV infection, affecting cancer progression, contributing to peripheral neuropathy, and driving airway damage in pulmonary ischemia-reperfusion injury has been revealed through various studies. Understanding Ccr5 through genetic models like knockout or conditional knockout mouse models can provide valuable insights into these disease mechanisms, potentially leading to new therapeutic strategies for these diseases.
References:
1. Zhao, Li, Lai, Yu. . Monoclonal CCR5 Antibody: A Promising Therapy for HIV. In Current HIV research, 21, 91-98. doi:10.2174/1570162X21666230316110830. https://pubmed.ncbi.nlm.nih.gov/36927434/
2. Schlechter, Benjamin L, Stebbing, Justin. 2024. CCR5 and CCL5 in metastatic colorectal cancer. In Journal for immunotherapy of cancer, 12, . doi:10.1136/jitc-2023-008722. https://pubmed.ncbi.nlm.nih.gov/38719543/
3. Hemmatazad, Hossein, Berger, Martin D. 2021. CCR5 is a potential therapeutic target for cancer. In Expert opinion on therapeutic targets, 25, 311-327. doi:10.1080/14728222.2021.1902505. https://pubmed.ncbi.nlm.nih.gov/33719836/
4. Zhong, Shanshan, Liu, Fangxi, Giniatullin, Rashid, Guo, Qianqian, Zhao, Chuansheng. 2023. Blockade of CCR5 suppresses paclitaxel-induced peripheral neuropathic pain caused by increased deoxycholic acid. In Cell reports, 42, 113386. doi:10.1016/j.celrep.2023.113386. https://pubmed.ncbi.nlm.nih.gov/37948181/
5. Qi, Baowen, Fang, Qiang, Liu, Shiyuan, Huang, Yingchun, Shi, Jianyou. 2020. Advances of CCR5 antagonists: From small molecules to macromolecules. In European journal of medicinal chemistry, 208, 112819. doi:10.1016/j.ejmech.2020.112819. https://pubmed.ncbi.nlm.nih.gov/32947226/
6. Brelot, Anne, Chakrabarti, Lisa A. 2018. CCR5 Revisited: How Mechanisms of HIV Entry Govern AIDS Pathogenesis. In Journal of molecular biology, 430, 2557-2589. doi:10.1016/j.jmb.2018.06.027. https://pubmed.ncbi.nlm.nih.gov/29932942/
7. Santos, Jesse, Wang, Ping, Shemesh, Avishai, Greenland, John R, Calabrese, Daniel R. 2023. CCR5 drives NK cell-associated airway damage in pulmonary ischemia-reperfusion injury. In JCI insight, 8, . doi:10.1172/jci.insight.173716. https://pubmed.ncbi.nlm.nih.gov/37788115/
8. Fantuzzi, Laura, Tagliamonte, Maria, Gauzzi, Maria Cristina, Lopalco, Lucia. 2019. Dual CCR5/CCR2 targeting: opportunities for the cure of complex disorders. In Cellular and molecular life sciences : CMLS, 76, 4869-4886. doi:10.1007/s00018-019-03255-6. https://pubmed.ncbi.nlm.nih.gov/31377844/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen