C57BL/6JCya-Dcnem1flox/Cya
Common Name:
Dcn-flox
Product ID:
S-CKO-02007
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dcn-flox
Strain ID
CKOCMP-13179-Dcn-B6J-VA
Gene Name
Product ID
S-CKO-02007
Gene Alias
DC; DSPG2; PG40; PGII; PGS2; SLRR1B
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dcnem1flox/Cya mice (Catalog S-CKO-02007) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105287
NCBI RefSeq
NM_001190451
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Overview of Gene Research
Dcn, short for Decorin, is a member of the small leucine-rich proteoglycan gene family. It is secreted from stromal fibroblasts and has non-cell-autonomous anti-cancer effects. Dcn is involved in multiple biological processes, such as regulating the extracellular matrix. It may interact with various signaling pathways like IL-6/STAT3/AUF1, TGF-β1/SMAD3, and is important in immune responses, cancer development, and tissue fibrosis [1,2].
In breast stromal fibroblasts, DCN down-regulation activates these cells and promotes their pro-carcinogenic effects through the IL-6/STAT3/AUF1 signaling. DCN-deficient fibroblasts enhance angiogenesis, orthotopic tumor growth, and increase the resistance of breast cancer cells to cisplatin [1]. In renal cell carcinoma, DCN acts as a tumor suppressor. DCN overexpression inhibits cell proliferation and metastasis both in vitro and in vivo by upregulating P21 and E-cadherin [4]. In corneal fibrosis and neovascularization, AAV5-DCN gene therapy can effectively reduce these conditions in rabbit eyes [3].
In conclusion, Dcn plays crucial roles in cancer and tissue-related diseases. Studies on Dcn, especially through in vivo models, have revealed its anti-carcinogenic and anti-fibrotic properties, providing potential therapeutic targets for breast cancer, renal cell carcinoma, and corneal diseases.
References：
1. Aljagthmi, Wafaa A, Alasmari, Manal A, Daghestani, Maha H, Al-Mohanna, Falah H, Aboussekhra, Abdelilah. 2024. Decorin (DCN) Downregulation Activates Breast Stromal Fibroblasts and Promotes Their Pro-Carcinogenic Effects through the IL-6/STAT3/AUF1 Signaling. In Cells, 13, . doi:10.3390/cells13080680. https://pubmed.ncbi.nlm.nih.gov/38667295/
2. Wang, Shanxi, Qu, Yunkun, Fang, Xuan, Wu, Hua, Liu, Yang. 2023. Decorin: a potential therapeutic candidate for ligamentum flavum hypertrophy by antagonizing TGF-β1. In Experimental & molecular medicine, 55, 1413-1423. doi:10.1038/s12276-023-01023-y. https://pubmed.ncbi.nlm.nih.gov/37394592/
3. Mohan, Rajiv R, Gupta, Suneel, Kumar, Rajnish, Chaurasia, Shyam S, Hesemann, Nathan P. 2024. Tissue-targeted and localized AAV5-DCN and AAV5-PEDF combination gene therapy abrogates corneal fibrosis and concurrent neovascularization in rabbit eyes in vivo. In The ocular surface, 32, 13-25. doi:10.1016/j.jtos.2024.01.001. https://pubmed.ncbi.nlm.nih.gov/38191093/
4. Xu, Yongcan, Xia, Qiuyuan, Rao, Qiu, Chen, Hui, Zhou, Xiaojun. 2015. DCN deficiency promotes renal cell carcinoma growth and metastasis through downregulation of P21 and E-cadherin. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37, 5171-83. doi:10.1007/s13277-015-4160-1. https://pubmed.ncbi.nlm.nih.gov/26547587/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen