C57BL/6JCya-S1pr1em1flox/Cya
Common Name
S1pr1-flox
Product ID
S-CKO-02136
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-13609-S1pr1-B6J-VA
When using this mouse strain in a publication, please cite “S1pr1-flox Mouse (Catalog S-CKO-02136) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
S1pr1-flox
Strain ID
CKOCMP-13609-S1pr1-B6J-VA
Gene Name
Product ID
S-CKO-02136
Gene Alias
S1p, Edg1, Lpb1, S1p1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000055676
NCBI RefSeq
NM_007901
Target Region
Exon 2
Size of Effective Region
~3.9 kb
Overview of Gene Research
S1pr1, short for sphingosine-1-phosphate receptor-1, is a G-protein coupled receptor belonging to the sphingosine-1-phosphate receptor subfamily. It binds to sphingosine 1-phosphate (S1P), a bioactive lysosphingolipid, and is involved in multiple biological processes. S1pr1 plays prominent roles in regulating endothelial cell cytoskeletal structure, cell migration, immunomodulation, vasculogenesis during embryogenesis, and T cell egress [4]. The S1P-S1PR1 signaling axis is also key in osteoimmunology, regulating both the immune system and bone remodeling, and can directly target osteoclastogenesis and osteogenesis [1].
In cancer research, S1pr1 has diverse functions. Deletion of S1pr1 in ovarian cancer cells inhibited proliferation and migration, promoted cell senescence, and sensitized cells to cisplatin chemotherapy, with the S1PR1-PDK1-LATS1/2-YAP pathway regulating cell senescence through a YAP-mediated feedback loop [2]. In lung adenocarcinoma, S1pr1 expression is downregulated, and overexpression of S1pr1 inhibits the proliferation, migration, invasion, and adhesion of cancer cells via the p-STAT1/miR-30c-5p/FOXA1 signaling pathway [3]. S1PR1-mediated tumorigenesis is supported by downstream effectors like STAT3, interleukin-6, and NF-κB networks, and upregulation of its signaling pathways in some tumor cells can lead to drug-resistant cancer cells, mainly through STAT3 activation [5]. In allogeneic hematopoietic cell transplantation, inhibition of Sphk1 or S1PR1 in the recipient substantially attenuated acute GVHD while retaining the graft-versus-leukemia effect, with the Sphk1/S1P/S1PR1 pathway differentially modulating the alloreactivity of CD4+ and CD8+ T cells [6].
In conclusion, S1pr1 is a crucial receptor involved in multiple biological processes, especially in immune-related and cancer-related functions. Gene-knockout and conditional-knockout mouse models, as well as other loss-of-function experiments, have provided important insights into its role in various diseases, such as cancer and graft-versus-host disease. Understanding S1pr1 and its associated pathways may offer new therapeutic opportunities for these diseases.
References：
1. Xiao, Lan, Zhou, Yinghong, Friis, Thor, Beagley, Kenneth, Xiao, Yin. 2019. S1P-S1PR1 Signaling: the "Sphinx" in Osteoimmunology. In Frontiers in immunology, 10, 1409. doi:10.3389/fimmu.2019.01409. https://pubmed.ncbi.nlm.nih.gov/31293578/
2. Tao, Yi-Ping, Zhu, Heng-Yan, Shi, Qian-Yuan, Cheng, Shu-Qun, Pan, Wei-Wei. 2023. S1PR1 regulates ovarian cancer cell senescence through the PDK1-LATS1/2-YAP pathway. In Oncogene, 42, 3491-3502. doi:10.1038/s41388-023-02853-w. https://pubmed.ncbi.nlm.nih.gov/37828220/
3. Chai, Yanfei, Xiang, Hong, Ma, Yuchao, Jin, Longyu, Lu, Hongwei. 2024. S1PR1 suppresses lung adenocarcinoma progression through p-STAT1/miR-30c-5 p/FOXA1 pathway. In Journal of experimental & clinical cancer research : CR, 43, 304. doi:10.1186/s13046-024-03230-5. https://pubmed.ncbi.nlm.nih.gov/39551792/
4. Anu, B, Namitha, N N, Harikumar, K B. 2021. S1PR1 signaling in cancer: A current perspective. In Advances in protein chemistry and structural biology, 125, 259-274. doi:10.1016/bs.apcsb.2020.12.006. https://pubmed.ncbi.nlm.nih.gov/33931142/
5. Rostami, Narges, Nikkhoo, Afshin, Ajjoolabady, Amir, Yousefi, Mehdi, Jadidi-Niaragh, Farhad. . S1PR1 as a Novel Promising Therapeutic Target in Cancer Therapy. In Molecular diagnosis & therapy, 23, 467-487. doi:10.1007/s40291-019-00401-5. https://pubmed.ncbi.nlm.nih.gov/31115798/
6. Tian, Linlu, Wu, Yongxia, Choi, Hee-Jin, Ogretmen, Besim, Yu, Xue-Zhong. 2022. S1P/S1PR1 signaling differentially regulates the allogeneic response of CD4 and CD8 T cells by modulating mitochondrial fission. In Cellular & molecular immunology, 19, 1235-1250. doi:10.1038/s41423-022-00921-x. https://pubmed.ncbi.nlm.nih.gov/36071219/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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