C57BL/6JCya-Fat1em1flox/Cya
Common Name:
Fat1-flox
Product ID:
S-CKO-02365
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fat1-flox
Strain ID
CKOCMP-14107-Fat1-B6J-VA
Gene Name
Product ID
S-CKO-02365
Gene Alias
2310038E12Rik; Fath; mFat1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fat1em1flox/Cya mice (Catalog S-CKO-02365) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098796
NCBI RefSeq
NM_001081286
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
FAT1, encoding a protocadherin, is one of the most frequently mutated genes in human cancers. It activates multiple signaling pathways like Wnt/β -catenin, Hippo, and MAPK/ERK, influencing cell proliferation, migration, and invasion. Genetic models, such as KO/CKO mouse models, are valuable for studying its functions [2].
In mouse models of skin squamous cell carcinoma and lung tumours, Fat1 deletion accelerates tumour initiation and malignant progression, promotes a hybrid epithelial-to-mesenchymal transition (EMT) phenotype, increases tumour stemness, and leads to spontaneous metastasis. Mechanistically, FAT1 loss of function activates a CAMK2-CD44-SRC axis promoting YAP1 nuclear translocation and ZEB1 expression for the mesenchymal state, and inactivates EZH2 to promote SOX2 expression for the epithelial state [1]. In ER + breast cancer, FAT1 loss-of-function mutations are linked to resistance to CDK4/6 inhibitors, as FAT1 loss elevates CDK6 through the Hippo pathway [3].
In conclusion, Fat1 is crucial in maintaining organ development and its abnormal expression is associated with tumorigenesis. Studies using Fat1 KO/CKO mouse models have revealed its roles in cancer initiation, progression, and drug resistance, providing insights into potential cancer therapies.
References:
1. Pastushenko, Ievgenia, Mauri, Federico, Song, Yura, Helmbacher, Francoise, Blanpain, Cédric. 2020. Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis. In Nature, 589, 448-455. doi:10.1038/s41586-020-03046-1. https://pubmed.ncbi.nlm.nih.gov/33328637/
2. Peng, Zizhen, Gong, Yanyu, Liang, Xiaoqiu. 2021. Role of FAT1 in health and disease. In Oncology letters, 21, 398. doi:10.3892/ol.2021.12659. https://pubmed.ncbi.nlm.nih.gov/33777221/
3. Li, Zhiqiang, Razavi, Pedram, Li, Qing, Reis-Filho, Jorge S, Chandarlapaty, Sarat. . Loss of the FAT1 Tumor Suppressor Promotes Resistance to CDK4/6 Inhibitors via the Hippo Pathway. In Cancer cell, 34, 893-905.e8. doi:10.1016/j.ccell.2018.11.006. https://pubmed.ncbi.nlm.nih.gov/30537512/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen