C57BL/6NCya-Fgf15em1flox/Cya
Common Name:
Fgf15-flox
Product ID:
S-CKO-02402
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fgf15-flox
Strain ID
CKOCMP-14170-Fgf15-B6N-VA
Gene Name
Product ID
S-CKO-02402
Gene Alias
FGF19; Fgf8a
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Fgf15em1flox/Cya mice (Catalog S-CKO-02402) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033389
NCBI RefSeq
NM_008003
Target Region
Exon 1~2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Fgf15, the rodent orthologue of human FGF19, is a hormone expressed in ileal enterocytes of the small intestine. It plays a crucial role in the bile acid-FXR-FGF15/19 signaling axis. Bile acids, upon reabsorption in the ileum and colon, activate the nuclear receptor Farnesoid X receptor (FXR) which forms a heterodimer with retinoid X receptor α (RXRα). This heterodimer in the distal ileum regulates Fgf15 production. Fgf15 then travels via the enterohepatic circulation to activate the hepatic FGF receptor 4 (FGFR4)-β-klotho receptor complex, regulating gene expression involved in cholesterol, bile acid, and lipid metabolism, as well as cell proliferation [1].
Intestine-derived Fgf15-knockout (FGF15INT-KO) mice studies showed that these mice had increased systemic bile acid levels but decreased cholesterol levels when exposed to an obesogenic diet, indicating that gut-derived Fgf15 plays a primary role in regulating bile acid and cholesterol metabolism. However, control and FGF15INT-KO mice gained similar body weight and adiposity and did not show genotype-specific differences in glucose, mixed meal, pyruvate, and glycerol tolerance, suggesting that intestinal Fgf15 is not essential for energy and glucose balance [2]. Mice lacking FGF15 did not develop cardiac hypertrophy in response to high-fat diet, isoproterenol, or cold exposure, with lower heart weight/tibia length ratio and cardiomyocyte area under hypertrophy-inducing conditions compared to wild-type mice, and down-regulation of genes involved in fatty acid metabolism, revealing an Fgf15-mediated gut-to-heart axis in cardiac hypertrophy development and regulation of fatty acid metabolism in the myocardium [3].
In conclusion, Fgf15 is essential for regulating bile acid and cholesterol metabolism, and also plays a role in cardiac hypertrophy development. Gene knockout mouse models have been instrumental in uncovering these functions, providing insights into its potential as a therapeutic target for metabolic and cardiovascular diseases.
References:
1. Katafuchi, Takeshi, Makishima, Makoto. 2022. Molecular Basis of Bile Acid-FXR-FGF15/19 Signaling Axis. In International journal of molecular sciences, 23, . doi:10.3390/ijms23116046. https://pubmed.ncbi.nlm.nih.gov/35682726/
2. Bozadjieva-Kramer, Nadejda, Shin, Jae Hoon, Li, Ziru, Rothberg, Amy E, Seeley, Randy J. 2024. Intestinal FGF15 regulates bile acid and cholesterol metabolism but not glucose and energy balance. In JCI insight, 9, . doi:10.1172/jci.insight.174164. https://pubmed.ncbi.nlm.nih.gov/38587078/
3. Morón-Ros, Samantha, Blasco-Roset, Albert, Navarro-Gascon, Artur, Gavaldà-Navarro, Aleix, Planavila, Anna. 2023. A new FGF15/19-mediated gut-to-heart axis controls cardiac hypertrophy. In The Journal of pathology, 261, 335-348. doi:10.1002/path.6193. https://pubmed.ncbi.nlm.nih.gov/37650293/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen