C57BL/6JCya-Slc6a13em1flox/Cya
Common Name
Slc6a13-flox
Product ID
S-CKO-02546
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14412-Slc6a13-B6J-VA
Status
When using this mouse strain in a publication, please cite “Slc6a13-flox Mouse (Catalog S-CKO-02546) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Slc6a13-flox
Strain ID
CKOCMP-14412-Slc6a13-B6J-VA
Gene Name
Product ID
S-CKO-02546
Gene Alias
GAT3, Gat2, Gabt3
Background
C57BL/6JCya
Gene Full Name
solute carrier family 6 (neurotransmitter transporter, GABA), member 13
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 6 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000064580
NCBI Transcript ID
NM_144512
Target Region
Exon 5~7
Size of Effective Region
~2.5 kb
Overview of Gene Research
Slc6a13, also known as GABA transporter 2 (GAT-2), is a member of the neurotransmitter transporter family. It is involved in the transport of γ -amino butyric acid (GABA), with a proposed ion/substrate coupling stoichiometry of 3 Na+: 1 Cl-: 1 GABA [5]. It also has the ability to transport hypotaurine [4]. These transport functions play important roles in processes such as maintaining neurotransmitter homeostasis and cellular antioxidant defense.
In Slc6a13 -deficient (Slc6a13-/-; KO) mice, resistance to Pasteurella multocida infection was observed. This was associated with reduced inflammation, as glycine levels increased in the lungs of KO mice, and glycine supplementation alleviated infection-induced inflammation by blocking inflammasome activation [1]. In another study, Slc6a13 deficiency promoted Th17 cell differentiation in vitro and Th17 responses in a mouse model of intestinal infection and inflammation through activation of GABA-mTOR signaling [2]. Additionally, it was found that GABA transporter 2, encoded by Slc6a13, acts downstream of histone methyltransferase Ezh2 to control axon regeneration in mice [3].
In conclusion, Slc6a13 plays essential roles in immune responses, such as modulating inflammatory responses during bacterial infections and influencing Th17 cell differentiation. It also contributes to axon regeneration. Gene-knockout mouse models have been crucial in revealing these functions, providing insights into potential therapeutic targets for infectious diseases, immune-related disorders, and neural injury repair.
References：
1. He, Fang, Qiu, Yangyang, Wu, Xiaoyan, Li, Nengzhang, Peng, Yuanyi. 2022. Slc6a13 Deficiency Attenuates Pasteurella multocida Infection-Induced Inflammation via Glycine-Inflammasome Signaling. In Journal of innate immunity, 15, 107-121. doi:10.1159/000525089. https://pubmed.ncbi.nlm.nih.gov/35797984/
2. Ren, Wenkai, Liao, Yuexia, Ding, Xueyan, Zhu, Guoqiang, Yin, Yulong. 2018. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. In Mucosal immunology, 12, 531-544. doi:10.1038/s41385-018-0111-7. https://pubmed.ncbi.nlm.nih.gov/30523310/
3. Wang, Xue-Wei, Yang, Shu-Guang, Hu, Ming-Wen, Liu, Chang-Mei, Zhou, Feng-Quan. 2023. Histone methyltransferase Ezh2 coordinates mammalian axon regeneration via regulation of key regenerative pathways. In The Journal of clinical investigation, 134, . doi:10.1172/JCI163145. https://pubmed.ncbi.nlm.nih.gov/38015636/
4. Nishimura, Tomohiro, Higuchi, Kei, Yoshida, Yuko, Tomi, Masatoshi, Nakashima, Emi. . Hypotaurine Is a Substrate of GABA Transporter Family Members GAT2/Slc6a13 and TAUT/Slc6a6. In Biological & pharmaceutical bulletin, 41, 1523-1529. doi:10.1248/bpb.b18-00168. https://pubmed.ncbi.nlm.nih.gov/30270321/
5. Eskandari, Sepehr, Willford, Samantha L, Anderson, Cynthia M. . Revised Ion/Substrate Coupling Stoichiometry of GABA Transporters. In Advances in neurobiology, 16, 85-116. doi:10.1007/978-3-319-55769-4_5. https://pubmed.ncbi.nlm.nih.gov/28828607/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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