C57BL/6JCya-Gnmtem1flox/Cya
Common Name:
Gnmt-flox
Product ID:
S-CKO-02698
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gnmt-flox
Strain ID
CKOCMP-14711-Gnmt-B6J-VA
Gene Name
Product ID
S-CKO-02698
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gnmtem1flox/Cya mice (Catalog S-CKO-02698) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002846
NCBI RefSeq
NM_010321
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Gnmt, or glycine N-methyltransferase, is a key enzyme in one-carbon metabolism. It catalyzes the production of methylglycine (sarcosine) by taking a methyl group from S-adenosyl-L-methionine and methylating glycine, thus clearing methionine from the body [1,2]. This process is crucial in regulating methyl group availability in mammalian cells and is associated with pathways like autophagy, as well as cholesterol intracellular trafficking and mTOR signaling pathway [1,2].
Genetic models, especially knockout mouse models, have been instrumental in understanding Gnmt's role. Gnmt knockout mice develop liver injury, fibrosis, and hepatocellular carcinoma, indicating its importance in maintaining liver health [4]. In pancreatic cancer, Gnmt is markedly downregulated, suggesting its potential as a biomarker and therapeutic target [3]. In bladder cancer, higher Gnmt expression is associated with muscle invasion and metastasis, with urinary sarcosine levels potentially serving as a marker for muscle invasion [5]. In non-alcoholic fatty liver disease, miR-873-5p downregulates Gnmt, disrupting mitochondrial functionality [6].
In conclusion, Gnmt is essential in multiple biological processes, especially in maintaining liver homeostasis. Its role in diseases such as liver cancer, pancreatic cancer, and bladder cancer, as well as non-alcoholic fatty liver disease, has been revealed through gene knockout mouse models. These findings provide important insights into disease mechanisms and potential therapeutic targets related to Gnmt.
References:
1. Johnson, Adiv A, Cuellar, Trinna L. 2023. Glycine and aging: Evidence and mechanisms. In Ageing research reviews, 87, 101922. doi:10.1016/j.arr.2023.101922. https://pubmed.ncbi.nlm.nih.gov/37004845/
2. Yen, Chia-Hung, Lin, Yu-Ting, Chen, Heng-Li, Chen, Shih-Yin, Chen, Yi-Ming Arthur. 2012. The multi-functional roles of GNMT in toxicology and cancer. In Toxicology and applied pharmacology, 266, 67-75. doi:10.1016/j.taap.2012.11.003. https://pubmed.ncbi.nlm.nih.gov/23147572/
3. Heinzman, Zachary, Schmidt, Connor, Sliwinski, Marek K, Goonesekere, Nalin C W. 2021. The Case for GNMT as a Biomarker and a Therapeutic Target in Pancreatic Cancer. In Pharmaceuticals (Basel, Switzerland), 14, . doi:10.3390/ph14030209. https://pubmed.ncbi.nlm.nih.gov/33802396/
4. Lu, Shelly C, Mato, José M. . S-adenosylmethionine in liver health, injury, and cancer. In Physiological reviews, 92, 1515-42. doi:10.1152/physrev.00047.2011. https://pubmed.ncbi.nlm.nih.gov/23073625/
5. Kishi, Shingo, Mori, Shiori, Fujiwara-Tani, Rina, Nakagawa, Hidemitsu, Kuniyasu, Hiroki. 2023. ERVK13-1/miR-873-5p/GNMT Axis Promotes Metastatic Potential in Human Bladder Cancer though Sarcosine Production. In International journal of molecular sciences, 24, . doi:10.3390/ijms242216367. https://pubmed.ncbi.nlm.nih.gov/38003554/
6. Fernández-Tussy, Pablo, Fernández-Ramos, David, Lopitz-Otsoa, Fernando, Delgado, Teresa C, Martínez-Chantar, María L. 2019. miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease. In Molecular metabolism, 29, 40-54. doi:10.1016/j.molmet.2019.08.008. https://pubmed.ncbi.nlm.nih.gov/31668391/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen