C57BL/6JCya-Gpr50em1flox/Cya
Common Name:
Gpr50-flox
Product ID:
S-CKO-02726
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Gpr50-flox
Strain ID
CKOCMP-14765-Gpr50-B6J-VA
Gene Name
Product ID
S-CKO-02726
Gene Alias
H9
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr50em1flox/Cya mice (Catalog S-CKO-02726) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070449
NCBI RefSeq
NM_010340
Target Region
Exon 2
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Gpr50, an orphan G protein-coupled receptor highly homologous to melatonin receptors, is located on the X chromosome. It is involved in multiple biological processes, with its functions including the regulation of mitochondrial quality and quantity through mitophagy, participation in TGFβ signaling, and potential associations with synaptic plasticity and cognitive function. It also plays roles in endocrine and metabolic processes, and is linked to the regulation of oocyte maturation, among others [1,2,3,4,5].
In Gpr50-deficient mice, there is an accumulation of damaged mitochondria, disruption of oxidative phosphorylation (OXPHOS), leading to insufficient ATP production, excessive ROS generation, and ultimately impaired neuronal development. These mice also exhibit impaired social recognition, which can be rescued by prenatal treatment with mitoQ, indicating Gpr50's crucial role in maintaining mitochondrial OXPHOS in developing neurons [1]. In neural progenitor cells and tanycytes, Gpr50 knockout increases neurite outgrowth, cell motility and migration, suggesting that Gpr50 acts as an inhibitor of these processes via the G12/13 protein-RhoA pathway [6].
In conclusion, Gpr50 is essential for multiple biological functions, especially in neuronal development and mitochondrial function. The use of Gpr50 knockout mouse models has significantly contributed to understanding its role in neurological processes, highlighting its potential as a target for treating related disorders such as those involving impaired social recognition and abnormal neurite outgrowth.
References:
1. Liu, Ji-Chuan, Zhao, Xiu-Yun, Wu, Ming-Lei, Schachner, Melitta, Ma, Quan-Hong. 2024. GPR50 regulates neuronal development as a mitophagy receptor. In Cell death & disease, 15, 591. doi:10.1038/s41419-024-06978-y. https://pubmed.ncbi.nlm.nih.gov/39143050/
2. Ahmad, Raise, Wojciech, Stefanie, Jockers, Ralf. 2018. GPR50 in TGFβ signaling and breast cancer. In Oncoscience, 5, 157-158. doi:10.18632/oncoscience.433. https://pubmed.ncbi.nlm.nih.gov/30035175/
3. Li, Qifa, Zhang, Yue, Ge, Bi-Ying, Yang, Jin-Yi, Li, Shao. 2020. GPR50 Distribution in the Mouse Cortex and Hippocampus. In Neurochemical research, 45, 2312-2323. doi:10.1007/s11064-020-03089-w. https://pubmed.ncbi.nlm.nih.gov/32696324/
4. Yao, Zhenyu, Meng, Jun, Long, Jing, Zhang, Jian V, Ren, Pei-Gen. 2022. Orphan receptor GPR50 attenuates inflammation and insulin signaling in 3T3-L1 preadipocytes. In FEBS open bio, 13, 89-101. doi:10.1002/2211-5463.13516. https://pubmed.ncbi.nlm.nih.gov/36333974/
5. Chen, Yan, Zeng, Ruilin, Kou, Jiayi, Yin, Shi, Lan, Daoliang. 2022. GPR50 participates in and promotes yak oocyte maturation: A new potential oocyte regulatory molecule. In Theriogenology, 181, 34-41. doi:10.1016/j.theriogenology.2022.01.009. https://pubmed.ncbi.nlm.nih.gov/35063919/
6. Ahmad, Raise, Luka, Marine, Journe, Anne-Sophie, Prevot, Vincent, Jockers, Ralf. . Orphan GPR50 Restrains Neurite Outgrowth and Cell Migration by Activating the G12/13 Protein-RhoA Pathway in Neural Progenitor Cells and Tanycytes. In Journal of pineal research, 77, e70041. doi:10.1111/jpi.70041. https://pubmed.ncbi.nlm.nih.gov/40091563/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen