C57BL/6JCya-Grin1em1flox/Cya
Common Name
Grin1-flox
Product ID
S-CKO-02754
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14810-Grin1-B6J-VA
When using this mouse strain in a publication, please cite “Grin1-flox Mouse (Catalog S-CKO-02754) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Grin1-flox
Strain ID
CKOCMP-14810-Grin1-B6J-VA
Gene Name
Product ID
S-CKO-02754
Gene Alias
NR1, GluN1, Nmdar, NMD-R1, NMDAR1, M100174, Rgsc174, GluRzeta1, GluRdelta1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000028335
NCBI RefSeq
NM_008169
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Overview of Gene Research
GRIN1, encoding the obligatory GluN1 subunit of N-methyl-D-aspartate receptors (NMDARs), is crucial for synaptic transmission and plasticity, as well as neural circuit development [1]. NMDARs are a subtype of ionotropic glutamate receptors, and their function is integral to normal brain development and cognitive function [1].
In mice, disruption of Grin1 within corticotropin-releasing factor (CRF) neurons in the central amygdala enhanced fear memory, suggesting its role in fear-related behaviors [2]. Loss-of-function mutations in Grin1 in male Grin1-/-knockdown mice led to volume reductions in dopaminergic structures early in development, with delayed changes in limbic and white matter structures, and evidence of degenerating neurons in the mature brain, indicating ongoing cell loss due to NMDAR hypofunction [3]. Grin1 Y647S/+ mice, a model of GRIN1-related neurodevelopmental disorder, showed reduced whole-brain GluN1 levels, deficient NMDAR-mediated synaptic transmission in the hippocampus, and exhibited spontaneous seizures, altered vocalizations, muscle strength, sociability, and problem-solving [4].
In conclusion, Grin1 is essential for normal neural function, playing a key role in synaptic transmission, plasticity, and neural circuit development. Studies using gene-knockout (KO) mouse models have revealed its significance in various aspects of neural development, function, and disease, such as in fear-related behaviors, neurodegeneration, and neurodevelopmental disorders [2,3,4].
References：
1. Korinek, M, Candelas Serra, M, Abdel Rahman, Fes, Balik, A, Smejkalova, T. 2024. Disease-Associated Variants in GRIN1, GRIN2A and GRIN2B genes: Insights into NMDA Receptor Structure, Function, and Pathophysiology. In Physiological research, 73, S413-S434. doi:. https://pubmed.ncbi.nlm.nih.gov/38836461/
2. Gafford, Georgette, Jasnow, Aaron M, Ressler, Kerry J. 2014. Grin1 receptor deletion within CRF neurons enhances fear memory. In PloS one, 9, e111009. doi:10.1371/journal.pone.0111009. https://pubmed.ncbi.nlm.nih.gov/25340785/
3. Intson, Katheron, van Eede, Matthijs C, Islam, Rehnuma, Henkelman, R Mark, Ramsey, Amy J. 2019. Progressive neuroanatomical changes caused by Grin1 loss-of-function mutation. In Neurobiology of disease, 132, 104527. doi:10.1016/j.nbd.2019.104527. https://pubmed.ncbi.nlm.nih.gov/31299220/
4. Sullivan, Megan T, Tidball, Patrick, Yan, Yuanye, Collingridge, Graham L, Ramsey, Amy J. 2024. Grin1 Y 647 S/+ Mice: A Preclinical Model of GRIN1 -Related Neurodevelopmental Disorder. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.21.608984. https://pubmed.ncbi.nlm.nih.gov/39229143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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