C57BL/6JCya-Htr2aem1flox/Cya
Common Name:
Htr2a-flox
Product ID:
S-CKO-03004
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Htr2a-flox
Strain ID
CKOCMP-15558-Htr2a-B6J-VA
Gene Name
Product ID
S-CKO-03004
Gene Alias
5-HT-2; 5-HT-2A; E030013E04; Htr-2; Htr2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Htr2aem1flox/Cya mice (Catalog S-CKO-03004) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036653
NCBI RefSeq
NM_172812
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Htr2a, also known as the 5-hydroxytryptamine (serotonin) receptor 2A, is a crucial receptor in the serotonergic system. It plays important roles in fetal brain development, adult cognitive function, and is involved in many physiological processes related to the central nervous system [3,4,5]. Activation of Htr2a is key in the actions of hallucinogens, and it is also coupled to the Gq protein, which may trigger various downstream signaling pathways [2].
In a papain-induced lung inflammation mouse model, ILC2-specific depletion of Htr2a (Il5cre/+·Htr2aflox/flox mice) abolished the inhibitory effect of the HTR2A selective agonist (DOI) on ILC2s, indicating that Htr2a is involved in regulating the type 2 lung immune response [1].
In conclusion, Htr2a is essential for normal brain development and cognitive function, and is involved in the regulation of the immune response in the lung. Mouse models with Htr2a gene knockout or conditional knockout have provided important insights into its role in lung inflammation, suggesting Htr2a could be a potential target for treating type 2 lung inflammation [1].
References：
1. Wang, Zhishuo, Yan, Chenghua, Du, Qizhen, Zhu, Jinfang, Sun, Bing. 2023. HTR2A agonists play a therapeutic role by restricting ILC2 activation in papain-induced lung inflammation. In Cellular & molecular immunology, 20, 404-418. doi:10.1038/s41423-023-00982-6. https://pubmed.ncbi.nlm.nih.gov/36823235/
2. Kim, Kuglae, Che, Tao, Panova, Ouliana, Skiniotis, Georgios, Roth, Bryan L. . Structure of a Hallucinogen-Activated Gq-Coupled 5-HT2A Serotonin Receptor. In Cell, 182, 1574-1588.e19. doi:10.1016/j.cell.2020.08.024. https://pubmed.ncbi.nlm.nih.gov/32946782/
3. Serretti, Alessandro, Drago, Antonio, De Ronchi, Diana. . HTR2A gene variants and psychiatric disorders: a review of current literature and selection of SNPs for future studies. In Current medicinal chemistry, 14, 2053-69. doi:. https://pubmed.ncbi.nlm.nih.gov/17691947/
4. Norton, Nadine, Owen, Michael J. . HTR2A: association and expression studies in neuropsychiatric genetics. In Annals of medicine, 37, 121-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16026119/
5. Paquette, Alison G, Marsit, Carmen J. . The developmental basis of epigenetic regulation of HTR2A and psychiatric outcomes. In Journal of cellular biochemistry, 115, 2065-72. doi:10.1002/jcb.24883. https://pubmed.ncbi.nlm.nih.gov/25043477/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen