C57BL/6JCya-Slc6a4em1flox/Cya
Common Name:
Slc6a4-flox
Product ID:
S-CKO-03013
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc6a4-flox
Strain ID
CKOCMP-15567-Slc6a4-B6J-VA
Gene Name
Product ID
S-CKO-03013
Gene Alias
5-HTT; Htt; Sert
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc6a4em1flox/Cya mice (Catalog S-CKO-03013) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021195
NCBI RefSeq
NM_010484
Target Region
Exon 3~4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Slc6a4, encoding the serotonin transporter protein, is crucial for regulating serotonin (5-HT) levels, as the 5-HT transporter it codes for plays a key role in 5-HT reuptake [1,2,5]. Serotonin is involved in multiple physiological and psychological processes, and thus Slc6a4 is of great biological importance in relation to mental health and related functions [1,2,5].
Many studies focus on its polymorphisms and associations with various disorders. For example, the 5HTTLPR polymorphism in the 5' region of Slc6a4 has been widely studied in relation to psychiatric traits, with some suggesting associations with increased risk of posttraumatic stress disorder, higher likelihood for depression, obsessive-compulsive disorder, increased hostility and criminal behaviour [1,7]. Also, its DNA methylation has been associated with stress-related physiological changes; higher average levels of Slc6a4 DNAm were associated with higher average levels of salivary alpha-amylase (SAM axis) between-person, and with lower levels of salivary cortisol and alpha-amylase within-person [4]. In addition, in schizophrenia patients, the methylation level of Slc6a4 was significantly higher in women than in men, and higher in the schizophrenia group than in the control group, especially in the male subgroup [2]. However, meta-analysis did not find an association between Slc6a4 promoter and intron 2 variants and the risk of temporal lobe epilepsy, though the intron 2 VNTR seems to have opposite effects in different populations [3]. Also, no association was found between the Slc6a4 promoter polymorphism and alcohol dependence [6]. Moreover, the Slc6a4 short/short allele was found to increase anxiety symptoms in adolescents exposed to childhood food insecurity [8].
In conclusion, Slc6a4 is vital for regulating serotonin levels and is associated with numerous mental health-related conditions. Research on its polymorphisms, methylation, and gene-environment interactions has provided insights into the pathophysiology of various psychiatric and stress-related disorders, contributing to our understanding of the underlying mechanisms and potentially guiding future therapeutic strategies.
References：
1. Gelernter, Joel. 2014. SLC6A4 polymorphism, population genetics, and psychiatric traits. In Human genetics, 133, 459-61. doi:10.1007/s00439-013-1412-2. https://pubmed.ncbi.nlm.nih.gov/24385047/
2. Liu, Linlin, Hu, Yi, Lu, Yumei, Gao, Changqing, Nie, Shengjie. 2021. Sex-dependent DNA hypermethylation of SLC6A4 in patients with schizophrenia. In Neuroscience letters, 769, 136394. doi:10.1016/j.neulet.2021.136394. https://pubmed.ncbi.nlm.nih.gov/34910986/
3. Córdoba, Marta, Consalvo, Damián, Moron, Dolores Gonzalez, Kochen, Silvia, Kauffman, Marcelo Andrés. 2012. SLC6A4 gene variants and temporal lobe epilepsy susceptibility: a meta-analysis. In Molecular biology reports, 39, 10615-9. doi:10.1007/s11033-012-1949-5. https://pubmed.ncbi.nlm.nih.gov/23065262/
4. Stoffel, Martin, Rahn, Stefanie, Neubauer, Andreas B, Aguilar-Raab, Corina, Ditzen, Beate. 2023. Associations of SLC6A4 methylation with salivary cortisol, salivary alpha-amylase, and subjective stress in everyday life. In Psychoneuroendocrinology, 153, 106283. doi:10.1016/j.psyneuen.2023.106283. https://pubmed.ncbi.nlm.nih.gov/37196602/
5. Murphy, Dennis L, Moya, Pablo R. 2011. Human serotonin transporter gene (SLC6A4) variants: their contributions to understanding pharmacogenomic and other functional G×G and G×E differences in health and disease. In Current opinion in pharmacology, 11, 3-10. doi:10.1016/j.coph.2011.02.008. https://pubmed.ncbi.nlm.nih.gov/21439906/
6. Villalba, Karina, Attonito, Jennifer, Mendy, Angelico, Gasana, Janvier, Dorak, Tevfik M. . A meta-analysis of the associations between the SLC6A4 promoter polymorphism (5HTTLPR) and the risk for alcohol dependence. In Psychiatric genetics, 25, 47-58. doi:10.1097/YPG.0000000000000078. https://pubmed.ncbi.nlm.nih.gov/25710844/
7. Hande, Shyamala H, Krishna, Swathy M, Sahote, Komalroop Kaur, Ravidhran, Renuka, Das, Ranajit. . Population genetic variation of SLC6A4 gene, associated with neurophysiological development. In Journal of genetics, 100, . doi:. https://pubmed.ncbi.nlm.nih.gov/33764333/
8. Pilkay, S, Nolasco, M, Nunes, S, Trexler, N, Gump, B. . SLC6A4 gene variants moderate associations between childhood food insecurity and adolescent mental health. In Brain and behavior, 14, e3426. doi:10.1002/brb3.3426. https://pubmed.ncbi.nlm.nih.gov/38361316/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen