C57BL/6JCya-Cd47em1flox/Cya
Common Name:
Cd47-flox
Product ID:
S-CKO-03170
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cd47-flox
Strain ID
CKOCMP-16423-Cd47-B6J-VA
Gene Name
Product ID
S-CKO-03170
Gene Alias
9130415E20Rik; B430305P08Rik; IAP; Itgp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cd47em1flox/Cya mice (Catalog S-CKO-03170) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000084838
NCBI RefSeq
NM_010581
Target Region
Exon 2~3
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Cd47, also known as cluster of differentiation 47, is a protein expressed on the outer membrane of human cells. It binds to soluble and cell surface receptors, forming a pleiotropic receptor-multiligand interaction network [2]. Cd47 is involved in the CD47/SIRPα axis, where CD47 binds to SIRPα receptor on myeloid cells, delivering a “don't eat me” signal, which is crucial in immune responses, especially in tumor-associated immune regulation [1].
In cancer research, CD47 blockade has been investigated. In syngeneic immunocompetent mouse tumor models, the therapeutic effects of CD47 blockade depend on dendritic cell-mediated cross-priming of T cell responses rather than macrophage phagocytosis as previously thought. The antitumor effects also require the cytosolic DNA sensor STING in CD11c + cells [4]. In glioblastoma, inhibition of fatty acid oxidation (FAO) reduces CD47-mediated immune evasion, as FAO-derived acetyl-CoA upregulates CD47 transcription via NF-κB/RelA acetylation [3].
In conclusion, Cd47 plays a vital role in immune regulation, especially in the context of cancer immune evasion. Studies using mouse models have revealed its complex role in tumor-immune interactions, highlighting its potential as a therapeutic target for cancer immunotherapy. The understanding of how Cd47 functions in these models can help in developing more effective cancer treatment strategies [1,3,4].
References:
1. van Duijn, Anneloes, Van der Burg, Sjoerd H, Scheeren, Ferenc A. . CD47/SIRPα axis: bridging innate and adaptive immunity. In Journal for immunotherapy of cancer, 10, . doi:10.1136/jitc-2022-004589. https://pubmed.ncbi.nlm.nih.gov/35831032/
2. Isenberg, Jeffrey S, Montero, Enrique. . Tolerating CD47. In Clinical and translational medicine, 14, e1584. doi:10.1002/ctm2.1584. https://pubmed.ncbi.nlm.nih.gov/38362603/
3. Jiang, Nian, Xie, Bowen, Xiao, Wenwu, Chen, Hong-Wu, Li, Jian Jian. 2022. Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion. In Nature communications, 13, 1511. doi:10.1038/s41467-022-29137-3. https://pubmed.ncbi.nlm.nih.gov/35314680/
4. Liu, Xiaojuan, Pu, Yang, Cron, Kyle, Fu, Yang-Xin, Xu, Meng Michelle. 2015. CD47 blockade triggers T cell-mediated destruction of immunogenic tumors. In Nature medicine, 21, 1209-15. doi:10.1038/nm.3931. https://pubmed.ncbi.nlm.nih.gov/26322579/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen