C57BL/6JCya-Meis1em1flox/Cya
Common Name:
Meis1-flox
Product ID:
S-CKO-03704
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Meis1-flox
Strain ID
CKOCMP-17268-Meis1-B6J-VA
Gene Name
Product ID
S-CKO-03704
Gene Alias
C530044H18Rik; Evi8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Meis1em1flox/Cya mice (Catalog S-CKO-03704) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000185131
NCBI RefSeq
NM_010789
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Meis1, or myeloid ecotropic viral integration site 1, belongs to the homeobox containing transcriptional regulatory network (HOX). It encodes a transcription factor essential during development and is involved in multiple intracellular signaling pathways [1,3,5]. Meis1 has been associated with various biological processes and diseases, such as hematopoiesis, embryonic patterning, eye development, and diseases like restless legs syndrome (RLS) and cancers [1,3,4,6].
In RLS, GWAS identified risk variants in non-coding regions of Meis1. Mouse studies showed that heterozygous Meis1 inactivation causes hyperactivity at the onset of the inactive period, mimicking human RLS, and reveals its effect on the dopaminergic system [1,7]. In colorectal cancer, downregulation of Meis1 mediated by ELFN1-AS1/EZH2/DNMT3a axis promotes tumorigenesis and oxaliplatin resistance, while Meis1 overexpression impairs cell viability and enhances oxaliplatin sensitivity [2]. In the context of cardiovascular regeneration, inhibition of Meis1 expression increases neonatal mouse cardiomyocyte proliferative capacity, while overexpression reduces the length of the proliferative window [3].
In summary, Meis1 plays crucial roles in development, cell proliferation, and differentiation, and is implicated in diseases like RLS and cancers. Mouse models, including gene knockout or conditional knockout models, have been instrumental in revealing these functions, providing insights into the underlying mechanisms and potential therapeutic targets for these disease areas.
References:
1. Sarayloo, Faezeh, Dion, Patrick A, Rouleau, Guy A. 2019. MEIS1 and Restless Legs Syndrome: A Comprehensive Review. In Frontiers in neurology, 10, 935. doi:10.3389/fneur.2019.00935. https://pubmed.ncbi.nlm.nih.gov/31551905/
2. Li, Yimin, Gan, Yaqi, Liu, Jiaxin, Shu, Guang, Yin, Gang. 2022. Downregulation of MEIS1 mediated by ELFN1-AS1/EZH2/DNMT3a axis promotes tumorigenesis and oxaliplatin resistance in colorectal cancer. In Signal transduction and targeted therapy, 7, 87. doi:10.1038/s41392-022-00902-6. https://pubmed.ncbi.nlm.nih.gov/35351858/
3. Paul, Swagatika, Zhang, Xiaonan, He, Jia-Qiang. 2019. Homeobox gene Meis1 modulates cardiovascular regeneration. In Seminars in cell & developmental biology, 100, 52-61. doi:10.1016/j.semcdb.2019.10.003. https://pubmed.ncbi.nlm.nih.gov/31623926/
4. Yao, Maozhong, Gu, Yong, Yang, Zhaoxin, Zhong, Keyan, Chen, Zhanjuan. 2021. MEIS1 and its potential as a cancer therapeutic target (Review). In International journal of molecular medicine, 48, . doi:10.3892/ijmm.2021.5014. https://pubmed.ncbi.nlm.nih.gov/34318904/
5. Jiang, Mingzhu, Xu, Shuang, Bai, Mi, Zhang, Aihua. 2020. The emerging role of MEIS1 in cell proliferation and differentiation. In American journal of physiology. Cell physiology, 320, C264-C269. doi:10.1152/ajpcell.00422.2020. https://pubmed.ncbi.nlm.nih.gov/33296285/
6. Meriç, Neslihan, Kocabaş, Fatih. . The Historical Relationship Between Meis1 and Leukemia. In Advances in experimental medicine and biology, 1387, 127-144. doi:10.1007/5584_2021_705. https://pubmed.ncbi.nlm.nih.gov/35304708/
7. Salminen, Aaro V, Lam, Daniel D, Winkelmann, Juliane. 2019. Role of MEIS1 in restless legs syndrome: From GWAS to functional studies in mice. In Advances in pharmacology (San Diego, Calif.), 84, 175-184. doi:10.1016/bs.apha.2019.03.003. https://pubmed.ncbi.nlm.nih.gov/31229170/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen