C57BL/6JCya-Mifem1flox/Cya
Common Name:
Mif-flox
Product ID:
S-CKO-03731
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mif-flox
Strain ID
CKOCMP-17319-Mif-B6J-VA
Gene Name
Product ID
S-CKO-03731
Gene Alias
DER6; GIF; Glif
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mifem1flox/Cya mice (Catalog S-CKO-03731) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038169
NCBI RefSeq
NM_010798
Target Region
Exon 1~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Macrophage migration inhibitory factor (MIF) is a pleiotropic pro-inflammatory cytokine. It was initially identified as a cytokine from activated T cells and is now known to be secreted by various cell types such as hematopoietic, epithelial, endothelial, mesenchymal, and neuronal cells [2,3]. MIF plays a critical role in innate and adaptive immunity, cell survival signaling, and can regulate glucocorticoid-mediated immunosuppression [1,3]. It acts via binding to receptors like CD74/CD44, CXCR2, CXCR4, and CXCR7, activating downstream pathways including ERK1/2, AMPK, and AKT [2].
In pre-clinical models, genetic depletion of myeloid-derived MIF in perioperative ischemic stroke mice with middle cerebral artery occlusion following a surgical insult led to a significant reduction in endothelial cell apoptosis and necroptosis, as well as blood-brain-barrier disruption [4]. In mouse models of allergic asthma, the lack of MIF caused an almost complete abrogation of mucus secretion, eosinophilic inflammation, and airway hyper-responsiveness [6]. In spondyloarthritis pre-clinical models, genetic deletion or blockade of MIF reduced the severity of inflammation [5].
In conclusion, MIF is a multifaceted cytokine involved in numerous biological processes and diseases. Model-based research, especially using MIF knockout or conditional knockout mouse models, has revealed its crucial roles in inflammation, cell death, and immune-related diseases such as ischemic brain injury, allergic asthma, and spondyloarthritis. These studies provide insights into potential therapeutic strategies targeting MIF for treating related diseases.
References:
1. Kang, Insoo, Bucala, Richard. 2019. The immunobiology of MIF: function, genetics and prospects for precision medicine. In Nature reviews. Rheumatology, 15, 427-437. doi:10.1038/s41584-019-0238-2. https://pubmed.ncbi.nlm.nih.gov/31197253/
2. Jankauskas, Stanislovas S, Wong, Dickson W L, Bucala, Richard, Djudjaj, Sonja, Boor, Peter. 2019. Evolving complexity of MIF signaling. In Cellular signalling, 57, 76-88. doi:10.1016/j.cellsig.2019.01.006. https://pubmed.ncbi.nlm.nih.gov/30682543/
3. Sumaiya, Krishnamoorthi, Langford, Dianne, Natarajaseenivasan, Kalimuthusamy, Shanmughapriya, Santhanam. 2021. Macrophage migration inhibitory factor (MIF): A multifaceted cytokine regulated by genetic and physiological strategies. In Pharmacology & therapeutics, 233, 108024. doi:10.1016/j.pharmthera.2021.108024. https://pubmed.ncbi.nlm.nih.gov/34673115/
4. Li, Yan, Zou, Chengyu, Chen, Chen, Yuan, Junying, Li, Peiying. 2023. Myeloid-derived MIF drives RIPK1-mediated cerebromicrovascular endothelial cell death to exacerbate ischemic brain injury. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2219091120. doi:10.1073/pnas.2219091120. https://pubmed.ncbi.nlm.nih.gov/36693098/
5. Wu, Brian, Nakamura, Akihiro. 2022. Deep Insight into the Role of MIF in Spondyloarthritis. In Current rheumatology reports, 24, 269-278. doi:10.1007/s11926-022-01081-7. https://pubmed.ncbi.nlm.nih.gov/35809213/
6. Bozza, Marcelo T, Lintomen, Leticia, Kitoko, Jamil Z, Paiva, Cláudia N, Olsen, Priscilla C. . The Role of MIF on Eosinophil Biology and Eosinophilic Inflammation. In Clinical reviews in allergy & immunology, 58, 15-24. doi:10.1007/s12016-019-08726-z. https://pubmed.ncbi.nlm.nih.gov/30680604/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen