C57BL/6JCya-Mdh1em1flox/Cya
Common Name:
Mdh1-flox
Product ID:
S-CKO-03770
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mdh1-flox
Strain ID
CKOCMP-17449-Mdh1-B6J-VA
Gene Name
Product ID
S-CKO-03770
Gene Alias
B230377B03Rik; KAR; MDH-s; MDHA; Mor-2; Mor2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mdh1em1flox/Cya mice (Catalog S-CKO-03770) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000239073
NCBI RefSeq
NM_008618
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Mdh1, short for malate dehydrogenase 1, is a key enzyme involved in the malate-aspartate shuttle and the tricarboxylic acid cycle [6]. It catalyzes the reversible oxidation of L-malate to oxaloacetate, playing essential roles in mitochondrial NADH supply for oxidative phosphorylation and thus is of great biological importance in energy metabolism [6].
In acute liver failure (ALF), deacetylation of Mdh1 at K118 promotes neutrophil extracellular trap (NET) formation, a novel mode of cell death, which in turn aggravates the progression of ALF [1,3,4]. In ALI, the citrate cycle pathway including Mdh1 is inactivated, and Mdh1 promotes the cell vitality of primary alveolar epithelial type II (AT2) cells by increasing glucose intake [2]. In pancreatic ductal adenocarcinoma (PDAC), Mdh1 is required for maintaining the levels of the autophagy initiator ULK1 during autophagosome formation [5]. In addition, MDH1 deficiency in humans is associated with early-onset severe encephalopathy [6].
In conclusion, Mdh1 is crucial in multiple biological processes such as energy metabolism, cell viability regulation, autophagy, and NET formation. Studies, especially those using relevant disease models like ALF, ALI, and PDAC, have revealed its important roles in these disease conditions. The understanding of Mdh1's functions in these models provides insights into the pathogenesis of related diseases, which may help develop new therapeutic strategies.
References:
1. Wang, Yukun, Shi, Chunxia, Guo, Jin, Zhang, Long, Gong, Zuojiong. 2024. IDH1/MDH1 deacetylation promotes acute liver failure by regulating NETosis. In Cellular & molecular biology letters, 29, 8. doi:10.1186/s11658-023-00529-7. https://pubmed.ncbi.nlm.nih.gov/38172700/
2. Hu, Mu, Yang, JieLai, Xu, Yang, Liu, Jiao. 2022. MDH1 and MDH2 Promote Cell Viability of Primary AT2 Cells by Increasing Glucose Uptake. In Computational and mathematical methods in medicine, 2022, 2023500. doi:10.1155/2022/2023500. https://pubmed.ncbi.nlm.nih.gov/36158123/
3. Wang, Yukun, Guo, Jin, Zhang, Danmei, Zhang, Xiaoya, Gong, Zuojiong. 2024. IDH1/MDH1 deacetylation promotes NETosis by regulating OPA1 and autophagy. In International immunopharmacology, 143, 113270. doi:10.1016/j.intimp.2024.113270. https://pubmed.ncbi.nlm.nih.gov/39353390/
4. Shi, Chunxia, Wang, Yukun, Guo, Jin, Zhang, Yanqiong, Gong, Zuojiong. 2024. Deacetylated MDH1 and IDH1 aggravates PANoptosis in acute liver failure through endoplasmic reticulum stress signaling. In Cell death discovery, 10, 275. doi:10.1038/s41420-024-02054-8. https://pubmed.ncbi.nlm.nih.gov/38851781/
5. New, Maria, Van Acker, Tim, Sakamaki, Jun-Ichi, Howell, Michael, Tooze, Sharon A. 2019. MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma. In Cancer research, 79, 1884-1898. doi:10.1158/0008-5472.CAN-18-2553. https://pubmed.ncbi.nlm.nih.gov/30765601/
6. Broeks, Melissa H, Shamseldin, Hanan E, Alhashem, Amal, Jans, Judith J M, Alkuraya, Fowzan S. 2019. MDH1 deficiency is a metabolic disorder of the malate-aspartate shuttle associated with early onset severe encephalopathy. In Human genetics, 138, 1247-1257. doi:10.1007/s00439-019-02063-z. https://pubmed.ncbi.nlm.nih.gov/31538237/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen