C57BL/6JCya-Nipsnap1em1flox/Cya
Common Name:
Nipsnap1-flox
Product ID:
S-CKO-03963
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nipsnap1-flox
Strain ID
CKOCMP-18082-Nipsnap1-B6J-VA
Gene Name
Product ID
S-CKO-03963
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nipsnap1em1flox/Cya mice (Catalog S-CKO-03963) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038570
NCBI RefSeq
NM_008698
Target Region
Exon 2~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Nipsnap1, short for 4-Nitrophenylphosphatase Domain and Non-Neuronal SNAP25-Like 1, is a gene with diverse functions. It localizes to the mitochondrial matrix and is involved in multiple biological pathways such as non-shivering thermogenesis, mitophagy, and cellular senescence regulation. It also plays a role in pain transmission and may be related to inflammatory pain and TRPV6 channel regulation [1,2,3,4,5].
In gene-knockout studies, Nipsnap1 knockout (N1-KO) mice are unable to sustain activated energy expenditure and have lower body temperature during extended cold challenges, indicating its importance in long-term thermogenic maintenance in brown adipose tissue (BAT) [1]. In zebrafish, lack of functional Nipsnap1 leads to reduced mitophagy in the brain and parkinsonian phenotypes [3]. In the context of liver fibrosis, Nipsnap1 overexpression improves mitophagy and attenuates extracellular matrix production, while interference with it disrupts the beneficial effect of SIRT3 on mitophagy and liver fibrosis alleviation [2]. NIPSNAP1-deficient mice show exacerbated inflammatory pain, suggesting its role in the pathogenesis of such pain [4].
In conclusion, Nipsnap1 is crucial for maintaining normal physiological functions. Its functions range from energy metabolism regulation in BAT to mitophagy in different tissues and pain-related processes. The use of gene-knockout models in mice and zebrafish has significantly contributed to understanding its role in diseases like non-shivering thermogenesis-related metabolic disorders, liver fibrosis, and inflammatory pain.
References:
1. Liu, Yang, Qu, Yue, Cheng, Chloe, Sanghera, Navneet, Barrow, Joeva J. 2023. Nipsnap1-A regulatory factor required for long-term maintenance of non-shivering thermogenesis. In Molecular metabolism, 75, 101770. doi:10.1016/j.molmet.2023.101770. https://pubmed.ncbi.nlm.nih.gov/37423391/
2. Li, Ruixi, Wang, Zhecheng, Wang, Yue, Zhao, Yan, Yao, Jihong. 2023. SIRT3 regulates mitophagy in liver fibrosis through deacetylation of PINK1/NIPSNAP1. In Journal of cellular physiology, 238, 2090-2102. doi:10.1002/jcp.31069. https://pubmed.ncbi.nlm.nih.gov/37417912/
3. Princely Abudu, Yakubu, Pankiv, Serhiy, Mathai, Benan John, Johansen, Terje, Simonsen, Anne. 2019. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. In Developmental cell, 49, 509-525.e12. doi:10.1016/j.devcel.2019.03.013. https://pubmed.ncbi.nlm.nih.gov/30982665/
4. Okamoto, Kazuya, Ohashi, Masaki, Ohno, Kana, Ito, Seiji, Okuda-Ashitaka, Emiko. 2016. Involvement of NIPSNAP1, a neuropeptide nocistatin-interacting protein, in inflammatory pain. In Molecular pain, 12, . doi:10.1177/1744806916637699. https://pubmed.ncbi.nlm.nih.gov/27030720/
5. Schoeber, Joost P H, Topala, Catalin N, Lee, Kyu Pil, Hoenderop, Joost G J, Bindels, René J M. 2008. Identification of Nipsnap1 as a novel auxiliary protein inhibiting TRPV6 activity. In Pflugers Archiv : European journal of physiology, 457, 91-101. doi:10.1007/s00424-008-0494-5. https://pubmed.ncbi.nlm.nih.gov/18392847/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen