C57BL/6NCya-Ints6em1flox/Cya
Common Name
Ints6-flox
Product ID
S-CKO-03995
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-18130-Ints6-B6N-VA
When using this mouse strain in a publication, please cite “Ints6-flox Mouse (Catalog S-CKO-03995) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ints6-flox
Strain ID
CKOCMP-18130-Ints6-B6N-VA
Gene Name
Product ID
S-CKO-03995
Gene Alias
2900075H24Rik, DICE1, Ddx26, HDB, Notch2l
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000053959
NCBI RefSeq
NM_008715
Target Region
Exon 5~6
Size of Effective Region
~2.7 kb
Overview of Gene Research
INTS6, also known as Deleted in cancer cells 1 (DICE1), is a subunit of the Integrator complex. This complex is involved in 3' end processing of small nuclear RNAs (snRNAs) and premature transcription termination of many protein-coding genes. INTS6 plays a role in regulating the activity of the Integrator complex, especially through its interaction with protein phosphatase 2A (PP2A), and is associated with pathways like cell cycle regulation and various cancer-related signaling pathways [1,3,4].
In colorectal cancer, INTS6 expression is increased, and its down-regulation induces G1/S-phase cell cycle arrest, suppressing cell growth, while over-expression has the opposite effect. It promotes cancer progression by activating AKT and ERK signaling [2].
In prostate cancer, exogenous re-expression of INTS6 in androgen-independent cell lines suppresses colony formation, arresting cells in the G1 phase, and is linked to Wnt signaling pathway regulation [5]. Also, in castration-resistant prostate cancer cells, activation of INTS6 by a promoter-targeted small RNA suppresses cell proliferation and motility by regulating β-catenin signaling [6].
In conclusion, INTS6 has a significant impact on cell cycle regulation and cancer-related signaling pathways. Its dysregulation is associated with the development and progression of colorectal and prostate cancers. Studies on INTS6, including those using loss-of-function models, help in understanding the molecular mechanisms of cancer development and may provide potential therapeutic targets.
References：
1. Fujiwara, Rina, Zhai, Si-Nan, Liang, Dongming, Yang, Li, Wilusz, Jeremy E. 2023. IntS6 and the Integrator phosphatase module tune the efficiency of select premature transcription termination events. In Molecular cell, 83, 4445-4460.e7. doi:10.1016/j.molcel.2023.10.035. https://pubmed.ncbi.nlm.nih.gov/37995689/
2. Ding, Xufen, Chen, Tianwei, Shi, Qian, Xie, Dong, Li, Jingjing. 2021. INTS6 promotes colorectal cancer progression by activating of AKT and ERK signaling. In Experimental cell research, 407, 112826. doi:10.1016/j.yexcr.2021.112826. https://pubmed.ncbi.nlm.nih.gov/34508742/
3. Vervoort, Stephin J, Welsh, Sarah A, Devlin, Jennifer R, Gardini, Alessandro, Johnstone, Ricky W. 2021. The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer. In Cell, 184, 3143-3162.e32. doi:10.1016/j.cell.2021.04.022. https://pubmed.ncbi.nlm.nih.gov/34004147/
4. Fianu, Isaac, Ochmann, Moritz, Walshe, James L, Urlaub, Henning, Cramer, Patrick. 2024. Structural basis of Integrator-dependent RNA polymerase II termination. In Nature, 629, 219-227. doi:10.1038/s41586-024-07269-4. https://pubmed.ncbi.nlm.nih.gov/38570683/
5. Filleur, Stephanie, Hirsch, Jennifer, Wille, Aline, Nelius, Thomas, Wieland, Ilse. 2009. INTS6/DICE1 inhibits growth of human androgen-independent prostate cancer cells by altering the cell cycle profile and Wnt signaling. In Cancer cell international, 9, 28. doi:10.1186/1475-2867-9-28. https://pubmed.ncbi.nlm.nih.gov/19906297/
6. Chen, Hong, Shen, Hai-Xiang, Lin, Yi-Wei, Liu, Ben, Xie, Li-Ping. 2018. Small RNA-induced INTS6 gene up-regulation suppresses castration-resistant prostate cancer cells by regulating β-catenin signaling. In Cell cycle (Georgetown, Tex.), 17, 1602-1613. doi:10.1080/15384101.2018.1475825. https://pubmed.ncbi.nlm.nih.gov/29895194/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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