C57BL/6JCya-Pnocem1flox/Cya
Common Name
Pnoc-flox
Product ID
S-CKO-04007
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18155-Pnoc-B6J-VA
When using this mouse strain in a publication, please cite “Pnoc-flox Mouse (Catalog S-CKO-04007) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pnoc-flox
Strain ID
CKOCMP-18155-Pnoc-B6J-VA
Gene Name
Product ID
S-CKO-04007
Gene Alias
N/OFQ, Npnc1, OFQ/N
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000224594
NCBI RefSeq
NM_001205075
Target Region
Exon 3
Size of Effective Region
~2.0 kb
Overview of Gene Research
Pnoc, also known as prepronociceptin, is a gene that plays a crucial role in energy homeostasis and feeding regulation. It is associated with pathways related to appetite control.
PNOC-expressing neurons in the arcuate nucleus of the hypothalamus (ARC) are part of a previously unrecognized GABAergic population distinct from well-defined feeding regulatory AgRP or POMC neurons [1]. These neurons arborize densely in the ARC and provide inhibitory synaptic input to nearby anorexigenic POMC neurons, promoting hyperphagia and obesity upon high-fat-diet (HFD) feeding [1].
Selective ablation of PNOCARC neurons promotes the activation of POMC neurons upon HFD exposure, reduces feeding, and protects from obesity, but it does not affect food intake or body weight under normal chow consumption [1]. Also, loss of leptin receptor (Lepr) expression in PNOC-expressing neurons in the ARC causes hyperphagia and obesity, while restoring Lepr expression in PNOC neurons on a Lepr-null obese background substantially reduces body weight [2].
In conclusion, Pnoc is essential in regulating appetite and body weight, especially in the context of high-fat-diet-induced obesity. The study of Pnoc using gene-knockout (KO) or conditional-knockout (CKO) mouse models has revealed its key role in these processes, providing potential targets for obesity therapeutics.
References：
1. Jais, Alexander, Paeger, Lars, Sotelo-Hitschfeld, Tamara, Kloppenburg, Peter, Brüning, Jens C. 2020. PNOCARC Neurons Promote Hyperphagia and Obesity upon High-Fat-Diet Feeding. In Neuron, 106, 1009-1025.e10. doi:10.1016/j.neuron.2020.03.022. https://pubmed.ncbi.nlm.nih.gov/32302532/
2. Solheim, Marie H, Stroganov, Sima, Chen, Weiyi, Steuernagel, Lukas, Brüning, Jens C. 2025. Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis. In Cell, , . doi:10.1016/j.cell.2025.04.001. https://pubmed.ncbi.nlm.nih.gov/40273910/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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