C57BL/6JCya-Oprl1em1flox/Cya
Common Name
Oprl1-flox
Product ID
S-CKO-04119
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18389-Oprl1-B6J-VA
When using this mouse strain in a publication, please cite “Oprl1-flox Mouse (Catalog S-CKO-04119) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Oprl1-flox
Strain ID
CKOCMP-18389-Oprl1-B6J-VA
Gene Name
Product ID
S-CKO-04119
Gene Alias
NOP, KOR3, ORGC, ORL1, Oprl, XOR1, morc, KOR-3, LC132, MOR-C
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000071585
NCBI RefSeq
NM_011012
Target Region
Exon 3~4
Size of Effective Region
~3.6 kb
Overview of Gene Research
Oprl1, encoding the nociceptin/orphanin FQ receptor (NOP), is a key regulator in multiple biological processes. The NOP receptor is involved in the nociceptin/Orphanin FQ (N/OFQ) system, which has a significant impact on hippocampal function, learning, memory, and emotional regulation [1,6]. It is also associated with the regulation of pain, reward, and stress-related responses [3,4,5].
In a mouse model combining adverse maternal environment (AME) and post-weaning Western diet (WD), an AME and an AME-WD significantly increased the total hippocampal expression of Oprl1 and variant V4 in male offspring, through an epigenetic mechanism. This altered expression may contribute to cognitive impairment in adult males [1]. In another study, in marchigian sardinian (msP) alcohol-preferring rats, stress led to decreased methylation of Oprl1 in the nucleus accumbens and increased alcohol intake, suggesting an epigenetic mechanism in the influence of psychosocial stress on alcohol consumption [2]. In human alcoholics, the expression of Oprl1 mRNA was decreased in the central amygdala, potentially related to the impairment of cognitive control over alcohol-seeking behavior [5].
In conclusion, Oprl1 plays a crucial role in cognitive function, alcohol-related behaviors, and may be involved in the response to stress. Mouse models have been instrumental in revealing its role in cognitive impairment associated with adverse early-life environments and its contribution to alcohol-related behaviors. These findings highlight the potential of Oprl1 as a target for treating related cognitive and substance-abuse disorders.
References：
1. Ke, Xingrao, Huang, Yingliu, Fu, Qi, Sampath, Venkatesh, Lane, Robert H. 2022. Adverse maternal environment alters Oprl1 variant expression in mouse hippocampus. In Anatomical record (Hoboken, N.J. : 2007), 306, 162-175. doi:10.1002/ar.25056. https://pubmed.ncbi.nlm.nih.gov/35983908/
2. Ruggeri, Barbara, Macare, Christine, Stopponi, Serena, Ciccocioppo, Roberto, Schumann, Gunter. 2017. Methylation of OPRL1 mediates the effect of psychosocial stress on binge drinking in adolescents. In Journal of child psychology and psychiatry, and allied disciplines, 59, 650-658. doi:10.1111/jcpp.12843. https://pubmed.ncbi.nlm.nih.gov/29197086/
3. Seo, Ean-Jeong, Efferth, Thomas, Panossian, Alexander. 2018. Curcumin downregulates expression of opioid-related nociceptin receptor gene (OPRL1) in isolated neuroglia cells. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 50, 285-299. doi:10.1016/j.phymed.2018.09.202. https://pubmed.ncbi.nlm.nih.gov/30466988/
4. Cunningham, Christopher W, Elballa, Waleed M, Vold, Stephanie U. 2019. Bifunctional opioid receptor ligands as novel analgesics. In Neuropharmacology, 151, 195-207. doi:10.1016/j.neuropharm.2019.03.006. https://pubmed.ncbi.nlm.nih.gov/30858102/
5. Kuzmin, Alexander, Bazov, Igor, Sheedy, Donna, Harper, Clive, Bakalkin, Georgy. 2009. Expression of pronociceptin and its receptor is downregulated in the brain of human alcoholics. In Brain research, 1305 Suppl, S80-5. doi:10.1016/j.brainres.2009.05.067. https://pubmed.ncbi.nlm.nih.gov/19501074/
6. Abdel-Mouttalib, Ouagazzal. 2015. Nociceptin/orphanin-FQ modulation of learning and memory. In Vitamins and hormones, 97, 323-45. doi:10.1016/bs.vh.2014.10.006. https://pubmed.ncbi.nlm.nih.gov/25677778/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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